Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses

Lee, Sanghoon; Ahsan, Nagib; Lee, Ki-Won; Kim, Do‐Hyun; Lee, Dong-Gi; Kwak, Sang‐Soo; Kwon, Suk‐Yoon; Kim, Tae‐Hwan; Lee, Byung-Hyun

doi:10.1016/j.jplph.2007.01.003

Cited by 310 publications

(175 citation statements)

References 60 publications

(69 reference statements)

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“…Increased basal expression of Cu/Zn SOD) and APX in 'Jonsok' is expected to contribute to increased abiotic tolerance. In one study (Lee et al, 2007) simultaneous overexpression of Cu/Zn and APX in tall fescue resulted in an increase tolerance to a wide range of abiotic stresses. In another study, comparison of chilling sensitive to a more tolerant maize (Zea mays) line suggested that the reduced activities of APX, catalase, and monodehydroascobate reductase may contribute to lower chilling tolerance at the early stages of development in maize (Hodges et al, 1997).…”

Section: Antioxidative and Detoxification Proteinsmentioning

confidence: 99%

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Koehler

Wilson²,

Goodpaster

et al. 2012

Plant Physiology

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To gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of octoploid strawberry (Fragaria 3 ananassa) cultivars that differ in freezing tolerance was conducted. Four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar ('Jonsok') and least-tolerant cultivar ('Frida') were compared with a goal to reveal how freezing tolerance is achieved in this distinctive overwintering structure and to identify potential cold-tolerance-associated biomarkers. Supported by univariate and multivariate analysis, a total of 63 spots from twodimensional electrophoresis analysis and 135 proteins from label-free quantitative proteomics were identified as significantly differentially expressed in crown tissue from the two strawberry cultivars exposed to 0-, 2-, and 42-d cold treatment. Proteins identified as cold-tolerance-associated included molecular chaperones, antioxidants/detoxifying enzymes, metabolic enzymes, pathogenesis-related proteins, and flavonoid pathway proteins. A number of proteins were newly identified as associated with cold tolerance. Distinctive mechanisms for cold tolerance were characterized for two cultivars. In particular, the 'Frida' cold response emphasized proteins specific to flavonoid biosynthesis, while the more freezing-tolerant 'Jonsok' had a more comprehensive suite of known stress-responsive proteins including those involved in antioxidation, detoxification, and disease resistance. The molecular basis for 'Jonsok'-enhanced cold tolerance can be explained by the constitutive level of a number of proteins that provide a physiological stress-tolerant poise. Strawberry (Fragaria 3 ananassa) cultivation predominates in regions with mild winters. In colder climates, overwintering hardiness is an essential trait for strawberry cultivation. Freezing injury of strawberry plants is one of the greatest factors in reducing crop yield and quality in temperate regions. Winter damage in Norway, for example, on average causes losses of 20%. Thus, production of cultivars with improved freezing hardiness is one of Norway's major objectives for their strawberry breeding programs. Improvement of cold hardiness is desirable for securing economic sustainability of the existing crops, and for expanding the growing regions of temperate fruit crops. Because strawberry is a representative species for the Rosaceae crops (e

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Section: Antioxidative and Detoxification Proteinsmentioning

confidence: 99%

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Koehler

Wilson²,

Goodpaster

et al. 2012

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…Some basic biochemical parameters such as membrane permeability (Farooq and Beg, 1980), glutathione concentration (Hoque et al, 2007) and peroxidase activity (Lee et al, 2007) were used to estimate plant sensitivity and tolerance to air pollutants. De Nicola et al (2011a) demonstrated that in urban environments, the photosynthetic performance with aging of leaves of Quercus ilex strongly decreased and that this was mainly due to impaired CO 2 assimilation caused by stomata occlusion.…”

Section: Introductionmentioning

confidence: 99%

PAHs accumulation on leaves of six evergreen urban shrubs: A field experiment

Fellet

Poščić

Licen

et al. 2016

Atmospheric Pollution Research

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“…Thus, Aono et al (1995) crossed transgenic tobacco plants overexpressing SOD or GR and demonstrated a higher level of tolerance to MV-induced oxidative stress in the hybrids, compared to that of either parent. Similarly, SOD and APX have proven an effective combination in transgenic tobacco (Kwon et al 2002;Kim et al 2005), potato (Tang et al 2006) and tall fescue (Lee et al 2007a). In one study with tobacco (Lee et al 2007b) three components of the ROS defence network (SOD, APX and DHAR) were shown to provide further improvements in tolerance to salinity and MV, in comparison with plants over-expressing only the first two of these.…”

Section: Introductionmentioning

confidence: 99%

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

et al. 2011

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Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerable cell compartment, the chloroplast, have been explored in tobacco. Several plastid transformants were generated which contained either a Nicotiana mitochondrial superoxide dismutase (MnSOD) or an Escherichia coli glutathione reductase (gor) gene. MnSOD lines had a three-fold increase in MnSOD activity, but interestingly a five to nine-fold increase in total chloroplast SOD activity. Gor transgenic lines had up to 6 times higher GR activity and up to 8 times total glutathione levels compared to wild type tobacco. Photosynthetic capacity of transplastomic plants, as measured by chlorophyll content and variable fluorescence of PSII was equivalent to non-transformed plants.The response of these transplastomic lines to several applied stresses was examined. In a number of cases improved stress tolerance was observed. Examples include enhanced methyl viologen (Paraquat)-induced oxidative stress tolerance in Mn-superoxidase dismutase overexpressing plants, improved heavy metal tolerance in glutathione reductase expressing lines, and improved tolerance to UV-B radiation in both sets of plants.

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Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses

Cited by 310 publications

References 60 publications

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

PAHs accumulation on leaves of six evergreen urban shrubs: A field experiment

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

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