Simultaneous production of citric acid and erythritol from crude glycerol by Yarrowia lipolytica Wratislavia K1

Abstract

This study shows a possible microbial process for utilization of crude glycerol generated by the biodiesel industry for citric acid and erythritol production. Simultaneous production of citric acid and erythritol under nitrogen-limited conditions with glycerol as the carbon source was achieved with an acetate negative mutant of Y. lipolytica Wratislavia K1 in fed-batch cultivations. The effect of the initial glycerol concentration (from 30–180 g dm−3) on the citrate and erythritol production was investigated. As a result of the experiments, maximum citric acid production (110 g dm−3) and a very high amount of erythritol (81 g dm−3) were determined after 168 h of fed-batch cultivation with the initial glycerol concentration of 150 g dm−3 and the total glycerol concentration of 250 g dm−3. In addition, the citric acid to isocitric acid ratio of the products from this strain was 35.5:1.

High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica

“…Effect of the medium pH on the production of erythritol Our recent investigations have shown that an acetatenegative mutant of Y. lipolytica Wratislavia K1, can produce simultaneously high amounts of erythritol and citric acid under nitrogen-limited conditions, with glycerol as the carbon source, at the pH of 5.5, which is optimal for citrate biosynthesis (Rymowicz et al 2008). The effect of the pH of the medium on the production of erythritol by this strain in a glycerol containing medium (total concentration 300 g l -1 ) was examined using media of a pH of 2.5-6.5.…”

“…A cost reduction in erythritol production can be achieved by using less expensive substrates. We have recently reported that an acetate-negative mutant of Y. lipolytica Wratislavia K1 has the ability of simultaneously producing high amounts of erythritol and citric acid in media containing glycerol, at pH 5.5 which is optimal for citric acid biosynthesis by Y. lipolytica (Rymowicz et al 2006(Rymowicz et al , 2008. In the current study, we evaluate the dynamics and yield of erythritol production from raw glycerol by the strain Wratislavia K1 at various pH, and thereafter investigate, in fed-batch experiments, the effect of the optimal pH on the production of erythritol by other strains belonging to the species Y. lipolytica.…”

Comparison of citric acid production from glycerol and glucose by different strains of Yarrowia lipolytica

“…The results of our earlier studies showed that acetate-negative mutant strains of Y. lipolytica (strains Wratislavia 1.31, Wratislavia AWG7, and Wratislavia K1), cultivated in medium containing glycerol, produced high amounts of citric acid and low quantities of by-product, e.g., isocitric acid. Additionally, we have found that some yeast strains may produce a considerable amount of other by-products, such as erythritol and mannitol on glycerol media (Rymowicz et al , 2008Rywińska et al 2009). …”

Film-Forming Starch Composites for Agricultural Applications

“…The data obtained are presented in Table 3. When the ratio of glycerol and urea in the composite was in the range of 3:0-3:1, pH of soil ranged from 6.01 to 6 3:0.6 6.01 ± 0.05 5.75 ± 0.10 5.61 ± 0.01 5.71 ± 0.01 6.08 ± 0.11 6.29 ± 0.09 6.24 ± 0.10 6.46 ± 0.02 3:0.8 6.09 ± 0.07 5.87 ± 0.06 5.71 ± 0.02 5.83 ± 0.05 6.24 ± 0.04 6.31 ± 0.10 6.55 ± 0.04 6.64 ± 0.06 3:1 6.06 ± 0.04 5.59 ± 0.28 5.84 ± 0.02 5.90 ± 0.08 6.37 ± 0.07 6.53 ± 0.12 6.66 ± 0.11 6.74 ± 0.11 3:2 6.11 ± 0.07 6.13 ± 0.08 6.36 ± 0.05 6.49 ± 0.05 6.90 ± 0.09 7.17 ± 0.09 7.28 ± 0.03 7.58 ± 0.03 3:3 6.08 ± 0.04 6.45 ± 0.06 6.33 ± 0.03 6.57 ± 0.02 7.12 ± 0.07 7.39 ± 0.11 7.74 ± 0.41 7.85 ± 0.24…”

“…While the search of applications for low-purity technical glycerol is an urgent problem. The attempts to use crude glycerol as an additive of animal fodder, as a raw material for the production of fuel and chemicals have been reported [3][4][5][6]. Increase of biodiesel production is accompanied by the accumulation of larger amounts of rapeseed cake.…”