Simultaneous qualitative and quantitative analyses of ursolic acid and oleanolic acid in Punica granatum L. (Pomegranate) flowers by high-performance thin-layer chromatography

Wu, Wenxia; Wang, Liwa; Tian, Shuge

doi:10.1007/s00764-021-00092-x

Cited by 10 publications

(2 citation statements)

References 20 publications

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“…Several studies employing high-performance thin-layer chromatography (HPTLC) [15][16][17][18][19] and highperformance liquid chromatography (HPLC) [20][21][22][23] to estimate these markers alone or in combination have been published. However, a thorough review of the literature found that no validated HPTLC approach for simultaneously estimating all three markers has been published.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of HPTLC Method for Simultaneous Estimation of Berberine, Gallic Acid and Ursolic Acid in a Polyherbal Blend

Chitlange¹,

Chandani²,

Gandhi³

et al. 2022

Bull. of the Kar. Univ. "Chem". Ser.

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A sensitive High-performance thin-layer chromatography method was developed for simultaneous estimation of berberine, gallic acid and ursolic acid in a polyherbal blend and validated as per ICH guidelines.Polyherbal blend was prepared using widely recommended herbal plants for platelet augmentation activity viz. Carica papaya, Berberis aristata, Ocimum Sanctum and Tinospora Cordifolia. The optimized separation was obtained with TLC aluminum plates pre-coated with silica gel G60 F254 as a stationary phase and a solvent system containing Toluene : Ethyl acetate : Methanol : Formic acid (3:3:0.2:0.1 v/v/v/v). Berberine and gallic acid were found to demonstrate linearity in the range of 1μg/band – 6μg/band and ursolic acid in the range of 20 μg/band – 100 μg/band with the regression coefficient in acceptable limits. The method was also found to be specific and precise one. The accuracy of the developed method at 80 %, 100 % and 120 % levels was found to be within limits and % RSD was found to be less than 2. LOD and LOQ of all three standards were also determined. Blend was also quantified for the amount of berberine, gallic acid and ursolic acid presence and was found to be 37.8 μg, 46.1 μg and 108 μg per mg, respectively.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of HPTLC Method for Simultaneous Estimation of Berberine, Gallic Acid and Ursolic Acid in a Polyherbal Blend

Chitlange¹,

Chandani²,

Gandhi³

et al. 2022

Bull. of the Kar. Univ. "Chem". Ser.

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“…Pomegranate flowers are flowers of deciduous shrubs or small trees Pomegranate , a plant of the genus Pomegranate [1] . According to relevant literature [2] , [3] , [4] , [5] , pomegranates are rich in phenols, tannins, sugars, pigments, and trace elements, and polyphenols are the most abundant. Crude pomegranate extracts have good pharmacological effects, such as antioxidant, anti-aging, hypoglycemic, hypotensive, hypolipidemic, and anti-atherosclerosis [6] , [7] , [8] .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous process optimization of ultrasound-assisted extraction of polyphenols and ellagic acid from pomegranate (Punica granatum L.) flowers and its biological activities

Jiang

Liu

et al. 2021

Ultrasonics Sonochemistry

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The effects of pomegranate (Punica granatum) on nonalcoholic fatty liver disease: A systematic review of in vivo interventional studies

Tangestani,

Jamshidi,

Farhadi

et al. 2024

Phytotherapy Research

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Nonalcoholic fatty liver disease (NAFLD) is a disorder in which excess fat accumulates in hepatocytes and can lead to serious complications. Oxidative stress is one of the leading causes of NAFLD. Pomegranates are considered antioxidant‐rich fruit. This systematic review study was aimed to investigate the impact of pomegranate on NAFLD. PubMed, Scopus, and Google Scholar databases/search engines (from inception up to July 2023) were searched for interventional studies (human and animal) that examined the effects of supplementation with different parts of pomegranate including fruits, peels, seeds, or flower on NAFLD outcomes. A total of 222 articles were retrieved following the initial search. After excluding duplicates, the title and abstract of 114 articles were screened. Afterward, irrelevant articles were removed and the full texts of the remaining 27 articles were reviewed. Eventually, 19 articles (16 animal and three human interventional studies) that met the inclusion criteria, published between 2009 and 2023, were included in this systematic review. Our study indicates the potential beneficial effects of different parts of pomegranate on the improvement of NAFLD. However, given that the majority of the included articles were animal studies, further investigations in the form of human clinical trials are warranted to suggest a clinical indication of such interventions.

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Simultaneous qualitative and quantitative analyses of ursolic acid and oleanolic acid in Punica granatum L. (Pomegranate) flowers by high-performance thin-layer chromatography

Cited by 10 publications

References 20 publications

Development and Validation of HPTLC Method for Simultaneous Estimation of Berberine, Gallic Acid and Ursolic Acid in a Polyherbal Blend

Development and Validation of HPTLC Method for Simultaneous Estimation of Berberine, Gallic Acid and Ursolic Acid in a Polyherbal Blend

Simultaneous process optimization of ultrasound-assisted extraction of polyphenols and ellagic acid from pomegranate (Punica granatum L.) flowers and its biological activities

The effects of pomegranate (Punica granatum) on nonalcoholic fatty liver disease: A systematic review of in vivo interventional studies

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