Simultaneous quantification and inhibitory effect on LDL oxidation of the traditional Korean medicine, Leejung-tang

Abstract

BackgroundLeejung-tang (LJT) is a traditional Korean herbal medicine for the treatment of gastrointestinal disorders. In this study, we performed quantification analysis of five marker components, liquiritin (1), ginsenoside Rg1 (2), ginsenoside Rb1 (3), glycyrrhizin (4), and 6-gingerol (5) in LJT using a high performance liquid chromatography-photodiode array (HPLC–PDA). In addition, we investigated the inhibitory effect on low-density lipoprotein (LDL) oxidation by the LJT sample.MethodsCompounds 1–5 were separated within 35 min using a Gemini C18 column. The mobile phase used gradient elution with 1.0% (v/v) aqueous acetic acid (A) and 1.0% (v/v) acetic acid in acetonitrile (B). The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 203 nm, 254 nm, and 280 nm. The inhibitory effect on LDL oxidation conduct an experiment on thiobarbituric acid reactive substance (TBARS) assay, relative electrophoretic mobility (REM) assay, and electrophoresis of ApoB fragmentation of LJT.ResultsCalibration curves of compounds 1–5 showed good linearity (r2 ≥0.9995) in different concentration ranges. The recoveries of compounds 1–5 were in the range of 98.90–103.39%, with relative standard deviations (RSD) below 3.0%. The RSDs (%) of intra-day and inter-day precision were 0.10–1.08% and 0.29–1.87%, respectively. The inhibitory effect of LJT on Cu2+-induced LDL oxidation was defined by TBARS assay (IC50: 165.7 μg/mL) and REM of oxLDL (decrease of 50% at 127.7 μg/mL). Furthermore LJT reduced the fragmentation of ApoB of oxLDL in a dose-dependent manner.ConclusionsThe established HPLC-PDA method will be helpful to improve quality control of LJT. In addition, LJT is a potential LDL oxidation inhibitor.

Direct Infusion MS-Based Lipid Profiling Reveals the Pharmacological Effects of Compound K-Reinforced Ginsenosides in High-Fat Diet Induced Obese Mice

“…LPCs, major lipid constituents of oxidized LDL, are generated via hydrolysis of the lipid components of oxidized LDL by lipoprotein-associated phospholipase A2. , The levels of LPCs, including LPC 20:4 and LPC 22:6, increased on ingestion of an HFD but were significantly reduced in mouse serum if the diet contained compound K. Especially, the LPC 22:6 level did not differ significantly from normal controls under such circumstances (Figure ). Earlier studies found that LDL oxidation was inhibited by ginsenosides Rb1 and Rg1 . Therefore, our work highlights the fact that the reduced LPC 20:4 and LPC 22:6 levels in the HFD+CK group may be attributable to an effect of compound K, a metabolite of ginsenoside Rb1.…”

“…Earlier studies found that LDL oxidation was inhibited by ginsenosides Rb1 and Rg1. 57 Therefore, our work highlights the fact that the reduced LPC 20:4 and LPC 22:6 levels in the HFD+CK group may be attributable to an effect of compound K, a metabolite of ginsenoside Rb1.…”

Evaluation of Antioxidant and Cytotoxic Activity of Plants Allium Ponticum and Allium Saxatile, Growing in Georgia

“…After this period, the medium was removed, and the antioxidant activity was evaluated using the thiobarbituric acid reactive substances (TBARS) assay. 19 The cells were seeded on a 24-well plate with a 500 μL serum-free RPMI 1640 culture medium and were treated with LDL (200 µg/mL) and samples. TBARS were evaluated exactly as previously described referring to a Malone di-aldehyde standard.…”