Simultaneous quantification of five marker compounds of Betula utilis stem bark using a validated high‐performance thin‐layer chromatography method

Khan, Imran; Sangwan, Payare L.; Dhar, Jagdish K.; Koul, Surrinder

doi:10.1002/jssc.201100647

Cited by 20 publications

(11 citation statements)

References 23 publications

(17 reference statements)

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“…HPTLC method was validated according to International Council for Harmonisation (ICH) guidelines for linearity, specificity, sensitivity, accuracy, precision, robustness, and stability [10].…”

Section: Methodsmentioning

confidence: 99%

“…As this species possess diversified bioactive compounds; adulterations may cause variability in the active ingredients and influence the efficacy and safety of the drug. In recent years, high‐performance thin‐layer chromatography (HPTLC) emerged as a sophisticated analytical tool in quality control of complex plant matrices due to its low operating cost, less analysis time, high sample throughput, and simultaneous determination of several compounds at nanogram level [9,10]. It also enables to detect compounds having no UV absorption by post derivatization HPTLC [11].…”

Section: Introductionmentioning

confidence: 99%

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Computational and experimental validation of free radical scavenging properties of high‐performance thin‐layer chromatography quantified phenyl myristate in Homalium nepalense

Kanhar

Roy

Sahoo

2020

J of Separation Science

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Phenyl myristate was isolated from Homalium nepalense, which is known for its therapeutic virtues in traditional medicine. However, the study of radical scavengingcapacity of phenyl myristate is limited by its relatively low abundance in medicinal plants. We have studied the isolation, structure-elucidation, and bioactivities of high-performance thin-layer chromatography validated phenyl myristate from hydroalcohol-extract of bark of H. nepalense. The chemical structure of phenyl myristate was elucidated by spectroscopic methods. The chromatography was performed on high-performance thin-layer chromatography aluminum plates coated with silicagel 60 F 254 . Determination and quantitation of phenyl myristate were performed by densitometric-scanning at 254 nm (chloroform-methanol, 9:1, v/v; R f 0.49). The method was validated according to International Council for Harmonisation guidelines in terms of linearity, specificity, sensitivity, accuracy, precision, robustness, and stability. Linearity-range of phenyl myristate was 100-500 ng/5 µL with correlationcoefficient r 2 = 0.9997. Limits of detection and quantitation were 3.35 and 10.17 ng, respectively. Phenyl myristate showed significant free-radical-scavenging activities in 2,2˗diphenyl˗1˗picrylhydrazyl, oxygen-radical-absorbance-capacity, and ex vivo cellbased-antioxidant-protection-in-erythrocytes assays. Molecular-docking approach of phenyl myristate showed effective binding at active sites of human serum albumin (HSA) with the lowest binding energy (−8.4 kcal/mol) that was comparable with ascorbic acid (−5.0 kcal/mol). These studies provide mechanistic insight into the potential free radical scavenging activities of phenyl myristate.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Computational and experimental validation of free radical scavenging properties of high‐performance thin‐layer chromatography quantified phenyl myristate in Homalium nepalense

Kanhar

Roy

Sahoo

2020

J of Separation Science

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“…The fractions obtained after eluting the column with petroleum ether:ethyl acetate (85:15) showed an R f value similar to that of standard betulin, and these similar fractions were combined. The isolated compound was further characterized using spectral techniques and HPTLC (Pandey et al 2007;Khan et al 2012).…”

Section: Isolation and Characterizationmentioning

confidence: 99%

Bioactive non-sterol triterpenoid fromStreblus asper: microwave-assisted extraction, HPTLC profiling, computational studies and neuro-pharmacological evaluation in BALB/c mice

Verma

Tripathi

Saraf

2016

Pharmaceutical Biology

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Context: In folk medicine, the stem bark of Streblus asper Lour. (Moraceae) has been reported to possess anticonvulsant activity. However, no systematic/scientific validation is available. Objective This study explores the constituents in the stem bark, their biosassy-guided isolation and their efficacy in neuro-pharmacological disorders, for validating the traditional claims. Materials and methods: Microwave-assisted extraction (MAE) technique was employed to obtain the crude extract. The n-hexane, dichloromethane and aqueous fractions were prepared and phytoconstituents were ascertained by phytochemical tests. The isolated compound, betulin, was characterized by different physicochemical and spectral methods, including HPTLC. Finally, neuro-pharmacological evaluations were conducted at 100, 200, 400 mg/kg b.w., p.o. (25, 50, 100 mg/kg b.w. for betulin) doses in BALB/c mice. Results: The n-hexane fraction (400 mg/kg), and isolated compound betulin (100 mg/kg), showed maximum anticonvulsant activity in maximal electroshock (87.84% and 85.14% seizure inhibition), and isoniazid induced convulsion models (88.85% and 83.18% seizure inhibition), respectively. A dose-dependent attenuation of epileptic seizures was observed, probably through GABArgic mechanism of anticonvulsant action. Moreover, the antidepressant study was also conducted using behavioural models and the results expound that n-hexane and dichloromethane fractions (400 mg/kg) significantly reduced the duration of immobility, as compared to the control. Discussion and conclusion: This study reports some novel aspects like applying an environmentally benign/green approach of MAE, neuro-pharmacological screening and use of docking studies, for the first time, on the plant S. asper. The findings present a rational explanation for its use in traditional medicine, for the management of neuro-pharmacological disorders.ARTICLE HISTORY

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“…[12,13] Hence HPTLC is a globally accepted rational and practical solution to characterize the crude plant drug, pharmacologically active constituents, enriched standardized extracts, and their formulations. [14] The present work deals with the separation and quantification of five major amino acids in different medicinal plants, viz., Amoora rohitaka (Roxb. ), Adiantum capillus-veneris, Sphaeranthus hirtus Willd., Swertia chirata (Wall.…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantification of Amino Acids From Medicinally Important Plants by Using High-Performance Thin-Layer Chromatography

Rajurkar

Gaikwad

2014

Journal of Liquid Chromatography & Related Technologies

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& The present paper deals with simultaneous identification and quantitative determination of five amino acids, viz., L-lysine, L-threonine, L-valine, L-isoleucine, and L-tryptophan using highperformance thin-layer chromatographic (HPTLC) technique in five different medicinal plants, viz., Amoora rohitaka (Roxb.), Adiantum capillus-veneris, Sphaeranthus hirtus Willd., Swertia chirata (Wall.), and Malaxis acuminata. The separation was performed on TLC aluminium plates precoated with silica gel 60 F 254 . Good separation was achieved in the mobile phase of 1-butanol=glacial acetic acid=water (4:2:2) and densitometric determination of these compounds was carried out at 254 nm in absorbance mode. The results provide valuable information about the presence of essential amino acids. Threonine is found in all the analyzed samples in the range of 145 ng=spot to 343 ng=spot. Adiantum capillus-veneris contains the highest amount of amino acid (1.57 mg=g) among the plants under study.

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Simultaneous quantification of five marker compounds of Betula utilis stem bark using a validated high‐performance thin‐layer chromatography method

Cited by 20 publications

References 23 publications

Computational and experimental validation of free radical scavenging properties of high‐performance thin‐layer chromatography quantified phenyl myristate in Homalium nepalense

Computational and experimental validation of free radical scavenging properties of high‐performance thin‐layer chromatography quantified phenyl myristate in Homalium nepalense

Bioactive non-sterol triterpenoid fromStreblus asper: microwave-assisted extraction, HPTLC profiling, computational studies and neuro-pharmacological evaluation in BALB/c mice

Identification and Quantification of Amino Acids From Medicinally Important Plants by Using High-Performance Thin-Layer Chromatography

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