Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method

Li, Lie; Zhang, Jinlan; Sheng, Yuxin; Guo, Dan; Wang, Qiao; Guo, Hongzhu

doi:10.1016/j.jpba.2004.12.002

Cited by 97 publications

(45 citation statements)

References 9 publications

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“…notoginseng contains ~12% total saponins. The three major types of saponins are ginsenosides (e.g., Rb1, Rg1, Rg3), notoginsenosides (e.g., R1, R2, R3) and jiaogulan glucosides (e.g., RVI) (30)(31)(32)(33). The structures of both notoginsenosides and ginsenosides exhibit a tetracyclic gonane steroid core.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng

Lee

Hsieh

et al. 2016

Oncology Reports

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Abstract. Panax notoginseng (P. notoginseng) and its components are used as traditional Chinese medicine for cardiovascular disease, although studies concerning the anti-metastatic properties of these compounds are limited. The goal of this study was to investigate the effects of notoginsenoside R1 (NGR1), an important compound derived from P. notoginseng, on the metastasis of human colorectal cancer (CRC). The migratory, invasive, and adhesive abilities of cultured human CRC cells (HCT-116) treated with NGR1 and expression of metastasis-associated regulatory molecules were assessed. The migratory and invasive abilities of the HCT-116 cells were reduced after treatment with 75, 150 or 300 µM NGR1 for 24 h. When HCT-116 cells were incubated with 150 or 300 µM NGR1 for 24 h, matrix metalloproteinase (MMP)-9 expression was reduced compared with that of the control group. In the adhesion reaction assays, treatment with 150 or 300 µM NGR1 led to significantly decreased adhesion of the HCT-116 cells to endothelial cells (EA.hy926 cells). Levels of integrin-1 protein were significantly decreased in the HCT-116 cells following treatment with 75, 150 or 300 µM NGR1, and levels of E-selectin and intercellular adhesion molecule 1 (ICAM-1) proteins were significantly decreased in the EA.hy926 cells treated with 75, 150 or 300 µM NGR1. Scanning electron microscopy examination indicated that HCT-116 cells treated with lipopolysaccharide (LPS) combined with 300 µM NGR1 exhibited a less flattened and retracted shape compared with cells treated with LPS alone, and this change in shape is characteristic of extravasation. Additionally, the transepithelial electrical resistance of the EA.hy926 endothelial cell monolayer increased after incubation with 150 or 300 µM NGR1 for 24 h. Overall, these results demonstrated the anti-metastatic properties of 150 or 300 µM NGR1, a compound that affects CRC metastasis by inhibiting cell migration, invasion, and adhesion and by regulating expression of metastasis-associated signalling molecules.

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Section: Discussionmentioning

confidence: 99%

Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng

Lee

Hsieh

et al. 2016

Oncology Reports

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“…Many studies showed that ginsenosides Rg 1 and Re, and ginsenosides Rb 2 and Rb 3 could not be well separated (Fuzzati et al, 1999;Kwon et al, 2001;Li et al, 2005;Wan et al, 2006a). We utilized a different gradient eluent ratio to separate saponins with similar characteristics and retention time, i.e.…”

Section: Methods Validationmentioning

confidence: 99%

“…Some studies have been conducted to determine saponins from the roots of P. notoginseng, and the main saponins in roots of P. notoginseng were assayed, including HPLC-UV (Li et al, 2005), HPLC-ELSD (Wan et al, 2006b), HPLC-MS (Liu et al, 2004) and micellar electrokinetic chromatography . However, for flower buds, only five saponins were determined by HPLC-ELSD, wherein ginsenosides Rg 1 , Re and F 2 and notoginsenoside R 1 were not well-analyzed in flower buds by the HPLC-ELSD method (Wan et al, 2006b), probably due to the low detection sensitivity of ELSD and the low contents of saponins in the flower buds of P. notoginseng (Wei and Du, 1996) …”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of saponins in flower buds of Panax notoginseng using high performance liquid chromatography

Gao

Dan

Zhao

et al. 2007

Biomedical Chromatography

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The flower buds of Panax notoginseng have been commonly used for the treatment of hypertension, vertigo, tinnitus and acute faucitis in China. The amount of total saponins in the flower buds is higher than in any other parts of P. notoginseng. However, the compositions of flower buds have not been quantified clearly until now. A sensitive and efficient high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for the first time to simultaneously quantify eight active saponins in the flower buds of P. notoginseng, including notoginsenoside R(1) and ginsenosides Rg(1), Re, Rb(1), Rb(2), Rb(3), Rd and F(2). The analysis was performed on a reversed-phase C(18) column with gradient elution of acetonitrile and 0.01% aqueous formic acid. The proposed method provided good linearity, reproducibility and sensitivity for the simultaneous quantification of the investigated saponins with overall intra- and inter-day precision and accuracy of better than 4.1% (RSD) and higher than 95% (accuracy), respectively. The recoveries for all the saponins determined were in the range 94.7-104.8% with RSD better than 3.1%. Using the optimized method, we were able to analyze samples from different villages of Wenshan Prefecture, China, which is helpful for quality control of flower buds of P. notoginseng.

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“…Commonly, QAMS was proceeded by high-performance liquid chromatography coupled with a UV-detector (HPLC-UV), which was the most frequently used method in the quality control of CHM. [ 2 , 3 ] The quantity assessment of Panax Ginseng and Panax notoginseng by ginsenosides (ginsenoside Rgl, Re, Rf, Rhl, Rbl, Rc, Rb2, Rb3, Rd) was performed successfully by this method. [ 4 ] At the same time, four flavones in Radix Scutellariae were also simultaneously determined by QAMS.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantitative analysis of main components in linderae reflexae radix with one single marker

Wang

Zhang²,

Sun

et al. 2016

Journal of Liquid Chromatography & Related Technologies

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Establish a quantitative analysis of multi-components by the single marker (QAMS) method for quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four main components in Linderae Reflexae Radix. Four main components of pinostrobin, pinosylvin, pinocembrin, and 3,5-dihydroxy-2-(1-p-mentheneyl)-trans-stilbene were selected as analytes to evaluate the quality by RP-HPLC coupled with a UV-detector. The method was evaluated by a comparison of the quantitative results between the external standard method and QAMS with a different HPLC system. The results showed that no significant differences were found in the quantitative results of the four contents of Linderae Reflexae Radix determined by the external standard method and QAMS (RSD <3%). The contents of four analytes (pinosylvin, pinocembrin, pinostrobin, and Reflexanbene I) in Linderae Reflexae Radix were determined by the single marker of pinosylvin. This fingerprint was the spectra determined by Shimadzu LC-20AT and Waters e2695 HPLC that were equipped with three different columns.

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Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method

Cited by 97 publications

References 9 publications

Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng

Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng

Simultaneous determination of saponins in flower buds of Panax notoginseng using high performance liquid chromatography

Simultaneous quantitative analysis of main components in linderae reflexae radix with one single marker

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