Simultaneous quantification of triterpenoid saponins in rat plasma by UHPLC–MS/MS and its application to a pharmacokinetic study after oral total saponin of Aralia elata leaves

Abstract

A rapid, sensitive, and reliable analytical ultra performance liquid chromatography with tandem mass spectrometry method was developed for the simultaneous determination of Aralia-saponin IV, 3-O-β-d-glucopyranosyl-(1→3)-β-d-glucopyranosyl-(1→3)-β-d-glucopyranosyl oleanolic acid 28-O-β-d-glucopyranoside, Aralia-saponin A and Aralia-saponin B after the oral administration of total saponin of Aralia elata leaves in rat plasma. Plasma samples were pretreated by protein precipitation with methanol. The analysis was performed on an ACQUITY UPLC HSS T3 column. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode using an electrospray ionization source with negative ionization mode. Under the experimental conditions, the calibration curves of four analytes had good linearity values (r > 0.991). The intra- and inter-day precision values of the four analytes were ≤ 11.6%, and the accuracy was between -6.2 and 4.2%.The extraction recoveries of four triterpenoid saponins were in the range of 84.06-91.66% (RSD < 10.5%), and all values of the matrix effect were more than 90.30%. The developed analytical method was successfully applied to pharmacokinetic study on simultaneous determination of the four triterpenoid saponins in rat plasma after oral administration of total saponin of Aralia elata leaves, which helps guiding clinical usage of Aralia elata leaves.

Effects of Gut Microbiota and Ingredient-Ingredient Interaction on the Pharmacokinetic Properties of Rotundic Acid and Pedunculoside

“…A longer mean T max and lower systemic exposure for PDC were observed compared with RA. This phenomenon may be explained by their different chemical structures, which the attachment of one sugar moiety remarkably reduced the rate of absorption and decreased the systemic exposure, and the molecular mass of PDC was about 650 Da, which was slightly greater than the favorable value [20,21].…”

Evolution of the adaptogenic concept from traditional use to medical systems: Pharmacology of stress‐ and aging‐related diseases

“…The absorption of any potential therapeutic is a critical consideration, especially for oral dosing. Many pharmacokinetic studies of other adaptogens, including clinical trials, have been performed 339,584–611 . Some of these studies provided evidence of the level and steady‐state concentration of active compounds in the blood of human subjects who received oral administration of the herbal drugs in therapeutic doses.…”

Protective Effects of Total Saponins of Aralia elata (Miq.) on Endothelial Cell Injury Induced by TNF-α via Modulation of the PI3K/Akt and NF-κB Signalling Pathways

“…Seem. (TAS), the main pharmacologically active ingredient extracted from AS stimulate heart activity, possess anti-myocardial ischaemic and anti-hypoxic activities, exhibit a strong anti-arrhythmic effect and exert protective effects against diabetic cardiomyopathy [8,9,10,11,12,13,14,15,16]. In addition, our group has studied Aralia elata (Miq.)…”