Simultaneous quantification of two active compounds in raw and honey-processed Radix Astragali by high-performance thin-layer chromatography

Shi, Yun; Ji, Jing; Yang, Xin; Shu, Yachun; Liu, Xiao; Jin, Jianfeng; Qin, Kunming; Li, Weidong

doi:10.1007/s00764-020-00031-2

Cited by 9 publications

(5 citation statements)

References 16 publications

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“… Chien, et al (2022) found that the roasting process increased formononetin content. Astragaloside I, astragaloside IV, isoastragaloside I and astragaloside II were lower in PAR than in RAR ( Dai, et al, 2020 ; Shi et al, 2020 ). Wu et al (2021) showed that polysaccharides in PAR were differed in molecular weight, monosaccharide composition, glycosidic bonds, and degree of branching.…”

Section: Introductionmentioning

confidence: 87%

RRLC-QTOF/MS-Based Metabolomics Reveal the Mechanism of Chemical Variations and Transformations of Astragali Radix as a Result of the Roasting Process

Huang

Sun

et al. 2022

Front. Chem.

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Astragali Radix (AR), which is extensively used as a healthy food supplement and medicinal herb, contains two forms of products corresponding to raw Astragalus Radix (RAR) and processed Astragali Radix (PAR), which was obtained by roasting. In this study, a non-targeted rapid resolution liquid chromatography coupled with quadruple time-of-flight mass spectrometry (RRLC-Q/TOF-MS) based metabolomics approach was developed to investigate the chemical changes of AR due to roasting. A total of 63 compounds were identified or tentatively identified. Among them, 23 isoflavonoids (composed of 12 isoflavones, eight pterocarpans, and three isoflavans) and six cycloastragenols were characterized as differential metabolites. Heatmap visualization and high-performance liquid chromatography coupled with photodiode array and evaporative light scattering detector (HPLC–PDA-ELSD) quantitative analysis revealed that malonyl isoflavonoids or cycloastragenols were at higher levels in RAR. These might be converted to corresponding acetyl isoflavonoids and cycloastragenols and related isoflavonoid glycosides during roasting. To prove this prediction, chemical conversion experiments on malonyl isoflavonoids and cycloastragenols were performed to confirm and clarify the chemical transformation mechanism.

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Section: Introductionmentioning

confidence: 87%

RRLC-QTOF/MS-Based Metabolomics Reveal the Mechanism of Chemical Variations and Transformations of Astragali Radix as a Result of the Roasting Process

Huang

Sun

et al. 2022

Front. Chem.

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“…Song et al [25] have compared the effects of different processing methods on the content of flavonoid compounds and found that the content of Cal-7-O-GLU, For-7-O-GLU, calycosin, and formononetin processed by SFRH showed a statistically significant decrease; however, calycosin content processed by SFRW increased as compared to NP. Shi et al [26] also determined astragaloside IV and Cal-7-O-GLU content in AR that were collected from different sources using the SFHR method and found that the content of these active compounds displayed a significant decrease in SFHR samples as compared to NP samples. A possible explanation for the difference in active constitutes content in AR between non-processed and processed subgroups is that flavonoids were unstable at higher temperatures and could transform into the aglycones form during the processing procedures [26].…”

Section: Effects Of Different Processing Methods On the Content Of As...mentioning

confidence: 99%

“…Shi et al [26] also determined astragaloside IV and Cal-7-O-GLU content in AR that were collected from different sources using the SFHR method and found that the content of these active compounds displayed a significant decrease in SFHR samples as compared to NP samples. A possible explanation for the difference in active constitutes content in AR between non-processed and processed subgroups is that flavonoids were unstable at higher temperatures and could transform into the aglycones form during the processing procedures [26]. Dai et al [9] have investigated the thermal stability of isoflavonoids in the water decoction and thus found that the content of calycosin 7-O-β-D-(6"-malony)-glucoside and 6"-O-malonylononin may degrade into Cal-7-O-GLU and For-7-O-GLU during the heating condition, further studies are needed to illustrate the relationship between the changes of active components content and the underlying transformation mechanisms in different processing methods.…”

Section: Effects Of Different Processing Methods On the Content Of As...mentioning

confidence: 99%

Effects of Physical Properties and Processing Methods on Astragaloside IV and Flavonoids Content in Astragali radix

Chien¹,

Yang²,

Chen

2022

Molecules

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The aim of this study was to investigate the effects of the physical properties (diameter size, powder particle size, composition of bark- and wood-tissue, and turnover rate) and processing methods on the content of active ingredients in Astragali radix (AR), a popular Chinese herbal medicine. The astragaloside IV and flavonoid contents increased with decreasing diameter size. Bark-tissue had significantly higher astragaloside IV and formononetin content than that in the wood-tissue. As a higher proportion of bark-tissue is associated with decreasing diameter, a strong correlation was also shown between bark- to wood-tissue ratio and active ingredients’ content. Furthermore, an increase in astragaloside IV content was observed in thin powder as compared to coarse powder ground from the whole root. However, this association between active ingredients’ content and powder particle size was abolished when isolating bark- and wood-tissue individually. Moreover, AR stir-frying with refined honey, a typical processing method of AR, increased formononetin content. The turnover rate of active constituents upon decoction ranged from 61.9–81.4%. Assessing the active constituent contents using its physical properties and processing methods allows for a more comprehensive understanding of optimizing and strengthening the therapeutic potentials of AR used in food and herbal supplements.

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“…Medicinal Astragalus membranaceus is the root of perennial herb A. membranaceus and A. membranaceus in Inner Mongolia [4]. It is mild in nature and sweet in taste.…”

Section: Introductionmentioning

confidence: 99%

The state of astragaloside IV research: A bibliometric and visualized analysis

Liu,

Ji,

Liu

et al. 2023

Fundamemntal Clinical Pharma

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BackgroundAstragaloside IV has emerged as a pharmaceutical monomer with great medical applications and potential. Astragaloside IV has many effects such as improving myocardial ischemia, cerebral ischemia–reperfusion injury, anti‐inflammatory, analgesic, antiviral, promoting lymphocyte proliferation, and antitumor effects. However, there are few bibliometric studies on astragaloside IV.ObjectivesWe aim to visualize the hotspots and trends in astragaloside IV research through bibliometric analysis to further understand the future development of basic and clinical research.Methods The articles and reviews on astragaloside IV were screened from the Web of Science Core Collection, and knowledge maps were generated using CiteSpace software. Bibliometric analysis was performed on 971 articles published from 1998 to 2022.ResultsThe number of articles on astragaloside IV increased yearly. These publications came from 42 countries/regions, with China being the largest. The primary research institutions were Shanghai University of Traditional Chinese Medicine and Guangzhou University of Traditional Chinese Medicine. Journal of Ethnopharmacology was the most studied journal and co‐cited journal. A total of 473 authors were included, among which Hongxin Wang had the highest number of publications and Zhang Wd had the highest total citation frequency. After analysis, the most common keywords are astragaloside IV, expression, and oxidative stress. Cardiovascular disease, cerebral ischemia, cancer, and kidney disease are current and developing research fields.ConclusionThis study used bibliometrics and visualization methods to analyze the research hotspots and trends of astragaloside IV. Astragaloside IV on ischemia–reperfusion injury, cancer, and tumor may become the focus of future research.

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Simultaneous quantification of two active compounds in raw and honey-processed Radix Astragali by high-performance thin-layer chromatography

Cited by 9 publications

References 16 publications

RRLC-QTOF/MS-Based Metabolomics Reveal the Mechanism of Chemical Variations and Transformations of Astragali Radix as a Result of the Roasting Process

RRLC-QTOF/MS-Based Metabolomics Reveal the Mechanism of Chemical Variations and Transformations of Astragali Radix as a Result of the Roasting Process

Effects of Physical Properties and Processing Methods on Astragaloside IV and Flavonoids Content in Astragali radix

The state of astragaloside IV research: A bibliometric and visualized analysis

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