Simultaneous quantitation of azole antifungals, antibiotics, imatinib, and raltegravir in human plasma by two-dimensional high-performance liquid chromatography–tandem mass spectrometry

Jourdil, Jean-François; Tonini, Julia; Stanke‐Labesque, Françoise

doi:10.1016/j.jchromb.2012.12.028

Cited by 70 publications

(58 citation statements)

References 55 publications

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“…The plasma VRC trough concentration was determined on samples handled just before the subsequent VRC administration. VRC C min were measured by a validated liquid chromatography-tandem mass spectrometry method (29). Briefly, after protein precipitation, samples were injected into a 2-dimensional chromatographic system.…”

Section: Methodsmentioning

confidence: 99%

Variability of Voriconazole Plasma Concentrations after Allogeneic Hematopoietic Stem Cell Transplantation: Impact of Cytochrome P450 Polymorphisms and Comedications on Initial and Subsequent Trough Levels

Gautier-Veyret

Fonrose

Tonini

et al. 2015

Antimicrob Agents Chemother

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f Voriconazole (VRC) plasma trough concentrations (C min ) are highly variable, and this could affect treatment efficacy and safety in patients undergoing allogeneic hematopoietic stem cell transplantation (AHSCT). We aimed to describe the intra-and interindividual variation of VRC C min throughout the course of VRC therapy and to identify the determinants of this variation. Clinical data, medications, and VRC C min (n ‫؍‬ 308) of 33 AHSCT patients were retrospectively collected. Cytochrome P450 (CYP450) genotypes of CYP2C19, CYP3A4, and CYP3A5 patients were retrospectively determined before allografting, and a combined genetic score was calculated for each patient. The higher the genetic score, the faster the metabolism of the patient. The VRC C min inter-and intraindividual coefficients of variation were 84% and 68%, respectively. The VRC dose (D) was correlated to VRC C min (r ‫؍‬ 0.412, P < 0.0001) only for oral administration. The administration route and the genetic score significantly affected the initial VRC C min . Considering oral therapy, patients with a genetic score of <2 had higher initial VRC C min /D than patients with a genetic score of >2 (P ‫؍‬ 0.009). Subsequent VRC C min remained influenced by the genetic score (P ‫؍‬ 0.004) but were also affected by pump proton inhibitor comedication (P < 0.0001). The high variability of VRC C min in AHSCT patients is partially explained by the route of administration, treatment with pump proton inhibitors, and the combined genetic score. This study suggests the interest in combined genetic score determination to individualize a priori the VRC dose and underlines the need for longitudinal therapeutic drug monitoring to adapt subsequent doses to maintain the VRC C min within the therapeutic range.

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Section: Methodsmentioning

confidence: 99%

Variability of Voriconazole Plasma Concentrations after Allogeneic Hematopoietic Stem Cell Transplantation: Impact of Cytochrome P450 Polymorphisms and Comedications on Initial and Subsequent Trough Levels

Gautier-Veyret

Fonrose

Tonini

et al. 2015

Antimicrob Agents Chemother

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“…The HPLC system was coupled to a triple quadrupole mass spectrometer and quantification was performed in SRM mode with a run time of 2.5 min and retention times of 1.6 min for all the compounds. Our method has advantages over the five studies that have quantified levofloxacin by LC-MS/MS [29][30][31][32][33]. The first study quantified levofloxacin in human urine.…”

Section: Resultsmentioning

confidence: 99%

“…The third study [29] quantified 20 anti-TB drugs (16 second line including levofloxacin and four first line drugs) in human plasma using LC-MS/ MS but had a long run time of 13 min and used moxifloxacin-d4 as an internal standard for levofloxacin. The fourth study [31] simultaneously quantified azole antifun- gals, antibiotics (levofloxacin and others using levofloxacin-…”

Section: Resultsmentioning

confidence: 99%

“…Different analytical methods such as capillary electro kinetic chromatography [13][14][15][16], solid phase spectrofluorimetry [17], and high performance liquid chromatography with fluorescent or UV detection [18][19][20][21][22][23][24][25][26][27] have been developed to measure the levofloxacin concentrations in plasma, urine, bile, faeces and other biological fluids [28]. To our knowledge, so far only five studies [29][30][31][32][33] have quantified levofloxacin concentrations by LC-MS/MS; however, among these studies some studies measured levofloxacin in human urine [27], other studies used plasma samples but did not provide description on run time and the ones that did had a relatively long run time and sample preparation time. Therefore, to maximize the efficiency of existing LC-MS/MS methods and further compliment TDM, we aimed to develop and validate a rapid, selective, simple and robust method without extensive sample processing and short run times, to quantify levofloxacin and its metabolite desmethyl-levofloxacin in the serum of MDR-TB patients.…”

Section: Introductionmentioning

confidence: 99%

“…The study by Jourdil et al [31] has advantage over two studies [29,33] in terms of using stable isotopically labeled levofloxacin internal standard. We have minimized the run time for levofloxacin and desmethyl-levofloxacin compared to the procedure described in the previous studies by Kim et al [29], Jourdil et al [31] and Lee et al [33] For sample preparation, all of these studies [29,31,33] used protein precipitation which favors pharmacokinetic monitoring but took approx. 11 min for protein precipitation.…”

mentioning

confidence: 99%

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Determination of levofloxacin in human serum using liquid chromatography tandem mass spectrometry

Ghimire¹,

Hateren²,

Vrubleuskaya³

et al. 2018

J Appl Bioanal

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A rapid liquid chromatography tandem-mass spectrometry method was developed for the determination of levofloxacin and its metabolite (desmethyl-levofloxacin) in human serum. Sample preparation was done using protein precipitation technique. Our method had a run time of 2.5 min and retention times of 1.6 min for all analytes. The standard curves were linear within the concentration range of 0.10 to 5.00 mg/L for levofloxacin and 0.10 to 4.99 mg/L for desmethyllevofloxacin; a correlation coefficient (R 2 ) of 0.999 and 0.998 respectively. The lower limit of quantification for both analytes was 0.10 mg/L. Within-day precision ranged from 1.4% and 2.4% for levofloxacin, 1.5% to 5% for desmethyl-levofloxacin and between-day precision ranged from 3.6% to 4.1% for levofloxacin and 0.0% to 3.3% for desmethyl-levofloxacin; whereas, accuracy ranged from 0.1% to 12.7% for levofloxacin and 0.2% to 15.6% for desmethyl-levofloxacin. This method could be a useful asset for routine therapeutic drug monitoring of levofloxacin in multi-drug resistant tuberculosis patients.

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An LC–MS/MS method for rapid and sensitive high‐throughput simultaneous determination of various protein kinase inhibitors in human plasma

Abdelhameed

Attwa

Kadi

2016

Biomedical Chromatography

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A reliable, high-throughput and sensitive LC-MS/MS procedure was developed and validated for the determination of five tyrosine kinase inhibitors in human plasma. Following their extraction from human plasma, samples were eluted on a RP Luna®-PFP 100 Å column using a mobile phase system composed of acetonitrile and 0.01 m ammonium formate in water (pH ~4.1) with a ratio of (50:50, v/v) flowing at 0.3 mL min . The mass spectrometer was operating with electrospray ionization in the positive ion multiple reaction monitoring mode. The proposed methodology resulted in linear calibration plots with correlation coefficients values of r = 0.9995-0.9999 from concentration ranges of 2.5-100 ng mL for imatinib, 5.0-100 ng mL for sorafenib, tofacitinib and afatinib, and 1.0-100 ng mL for cabozantinib. The procedure was validated in terms of its specificity, limit of detection (0.32-1.71 ng mL ), lower limit of quantification (0.97-5.07 ng mL ), intra- and inter assay accuracy (-3.83 to +2.40%) and precision (<3.37%), matrix effect and recovery and stability. Our results demonstrated that the proposed method is highly reliable for routine quantification of the investigated tyrosine kinase inhibitors in human plasma and can be efficiently applied in the rapid and sensitive analysis of their clinical samples.

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Simultaneous quantitation of azole antifungals, antibiotics, imatinib, and raltegravir in human plasma by two-dimensional high-performance liquid chromatography–tandem mass spectrometry

Cited by 70 publications

References 55 publications

Variability of Voriconazole Plasma Concentrations after Allogeneic Hematopoietic Stem Cell Transplantation: Impact of Cytochrome P450 Polymorphisms and Comedications on Initial and Subsequent Trough Levels

Variability of Voriconazole Plasma Concentrations after Allogeneic Hematopoietic Stem Cell Transplantation: Impact of Cytochrome P450 Polymorphisms and Comedications on Initial and Subsequent Trough Levels

Determination of levofloxacin in human serum using liquid chromatography tandem mass spectrometry

An LC–MS/MS method for rapid and sensitive high‐throughput simultaneous determination of various protein kinase inhibitors in human plasma

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