Simultaneous quantitation of dexamethasone palmitate and dexamethasone in human plasma by liquid chromatography/tandem mass spectrometry

Yang, Yan; Li, Hao; Gao, Kan; Liu, Mingyuan; Sun, Yan; Yan, Tingting; Fawcett, J. Paul; Chen, Yimin; Gu, Jingkai

doi:10.1016/j.jchromb.2007.11.033

Cited by 28 publications

(22 citation statements)

References 14 publications

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“…Proper investigation of matrix effects includes, in addition to the absolute matrix effect, the study of matrices from different sources [27,29,[69][70][71][72][73][74][75][76][77][78][79][80].…”

Section: Literature Overviewmentioning

confidence: 99%

Validation of bioanalytical LC–MS/MS assays: Evaluation of matrix effects

Eeckhaut

Lanckmans

Sarre

et al. 2009

Journal of Chromatography B

688

379

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Section: Literature Overviewmentioning

confidence: 99%

Validation of bioanalytical LC–MS/MS assays: Evaluation of matrix effects

Eeckhaut

Lanckmans

Sarre

et al. 2009

Journal of Chromatography B

688

379

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“…HPLC with tandem mass spectrometric detection (LC-MS/MS) has been demonstrated to provide high sensitivity for the quantitative determination of drugs and metabolites in biological fluids. Some LC-MS/MS methods for measuring Dex in biological samples have been reported [3][4][5][6], in which a relatively large volume of sample was required (>200 l). For Dex SP analysis, there is one published HPLC-UV-MS method that has been used to report the analysis of Dex SP in cochlear perilymph of guinea pigs [7], in which HPLC with UV detection was used for Dex SP quantification and MS for Dex SP identification.…”

Section: Introductionmentioning

confidence: 99%

Determination of dexamethasone and dexamethasone sodium phosphate in human plasma and cochlear perilymph by liquid chromatography/tandem mass spectrometry

Mei

Moore

Jensen

et al. 2011

Journal of Chromatography B

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“…28 The extract was first injected onto a Gemini column in the HPLC/UV system with gradient elution and UV detection. The cartridges were preconditioned with 5 mL of methanol followed by 5 mL of Milli-Q water.…”

Section: Solid Phase Extraction Of Plasma Samplesmentioning

confidence: 99%

Development and validation of spectrofluorimetric and LC–MS/MS methods for the determination of hesperidin in human plasma and pharmaceutical forms

Pavun

Dimitrić-Marković

Đurđević

et al. 2012

JSCS

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A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 μg mL -1 with a limit of detection, LOD, of 0.016 μg mL -1 and a limit of quantification, LOQ, of 0.049 μg mL -1 . Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 μg mL -1 . The LOD was 0.032 μg mL -1 while LOQ was 0.096 μg mL -1 . Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC-MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 μg mL -1 . The LOD was 0.01 μg mL -1 and the LOQ was 0.03 μg mL -1 . The linearity range for the determination of hesperidin in plasma was 0.02-10.00 μg mL -1 with an LOD 0.005 μg mL -1 and an LOQ of 0.015 μg mL -1 . The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories.

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Simultaneous quantitation of dexamethasone palmitate and dexamethasone in human plasma by liquid chromatography/tandem mass spectrometry

Cited by 28 publications

References 14 publications

Validation of bioanalytical LC–MS/MS assays: Evaluation of matrix effects

Validation of bioanalytical LC–MS/MS assays: Evaluation of matrix effects

Determination of dexamethasone and dexamethasone sodium phosphate in human plasma and cochlear perilymph by liquid chromatography/tandem mass spectrometry

Development and validation of spectrofluorimetric and LC–MS/MS methods for the determination of hesperidin in human plasma and pharmaceutical forms

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