Simultaneous quantitation of hydrochlorothiazide and metoprolol in human plasma by liquid chromatography–tandem mass spectrometry

Gao, Feng; Zhang, Mengliang; Cui, Xiaoling; Wang, Zhonghua; Sun, Yan; Gu, Jingkai

doi:10.1016/j.jpba.2009.12.012

Cited by 42 publications

(23 citation statements)

References 13 publications

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“…HCT and angiotensin-converting enzyme inhibitors (cilazapril, quinapril, ramipril and enalapril) were measured by square wave voltammetry, ultraviolet (UV) spectroscopy, and HPLC [10][11][12][13]. HCT and a β-blocker (atenolol, metoprolol, nebivolol) were quantified using HPLC with UV and tandem mass spectrometry [14][15][16][17]. HCT and a calcium channel blocker (amlodipine) were determined by HPLC [18].…”

Section: Fig 1 Chemical Structures Of Hydrochlorothiazide and Valsamentioning

confidence: 99%

Total error-based validation including the experimental design-based robustness evaluation of a stability-indicating method for the simultaneous quantification of hydrochlorothiazide and valsartan in tablet formulations

Elkarbane¹,

AL-Kamarany²,

Bouchafra

et al. 2015

Acta Chromatographica

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Summary.A gradient reversed phase high-performance liquid chromatography (RP-HPLC) method with ultraviolet (UV) detection to analyze hydrochlorothiazide (HCT) and valsartan (VS) simultaneously in a tablet formulation during forced degradation studies was developed. This method was validated using a novel approach, namely, the accuracy profile or total errors approach. The robustness of the method was evaluated using a Plackett-Burman design for eight factors. The algorithm of Dong was applied to determine the significant factor effects. The validation results showed that the method is precise (RSD: 1.14% for HCT and 0.43% for VS) and accurate (mean recovery: 99.90% for HCT and 99.98% for VS). On the other hand, the results of the robustness study showed that the type of column was the important factor which affects a number of responses, namely, the asymmetry factor (AF), retention time (RT), and resolution (RS). However, the assay results were not affected; therefore, the method can be considered robust. Finally, the method was applied to study the stability of HCT and VS under forced conditions. Significant results were obtained with basic hydrolysis, oxidation, and thermal stress, while the accelerated and acidic conditions did not affect the stability of HCT or VS.

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Section: Fig 1 Chemical Structures Of Hydrochlorothiazide and Valsamentioning

confidence: 99%

Total error-based validation including the experimental design-based robustness evaluation of a stability-indicating method for the simultaneous quantification of hydrochlorothiazide and valsartan in tablet formulations

Elkarbane¹,

AL-Kamarany²,

Bouchafra

et al. 2015

Acta Chromatographica

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“…These methods include liquid chromatography coupled with mass spectrometry (LC-MS/MS) [7][8][9][10][11][12][13][14][15][16][17], liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS) [18,19], gas chromatography coupled with mass spectrometry (GC-MS) [3,[20][21][22], high performance liquid chromatography (HPLC) [23,24], high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) [25,26], high performance liquid chromatography with fluorescence detection (HPLC-FLD) [2,27,28], high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV) [29], reversed-phase ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) [30].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Corina¹,

Hăncianu²,

Agoroaei³

et al. 2015

Acta Medica Marisiensis

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Arterial hypertension is a complex disease with many serious complications, representing a leading cause of mortality. Selective beta-blockers such as metoprolol and bisoprolol are frequently used in the management of hypertension. Numerous analytical methods have been developed for the determination of these substances in biological fluids, such as liquid chromatography coupled with mass spectrometry, gas chromatography coupled with mass spectrometry, high performance liquid chromatography. Due to the complex composition of biological fluids a biological sample pre-treatment before the use of the method for quantitative determination is required in order to remove proteins and potential interferences. The most commonly used methods for processing biological samples containing metoprolol and bisoprolol were identified through a thorough literature search using PubMed, ScienceDirect, and Willey Journals databases. Articles published between years 2005-2015 were reviewed. Protein precipitation, liquid-liquid extraction and solid phase extraction are the main techniques for the extraction of these drugs from plasma, serum, whole blood and urine samples. In addition, numerous other techniques have been developed for the preparation of biological samples, such as dispersive liquid-liquid microextraction, carrier-mediated liquid phase microextraction, hollow fiber-protected liquid phase microextraction, on-line molecularly imprinted solid phase extraction. The analysis of metoprolol and bisoprolol in human plasma, urine and other biological fluids provides important information in clinical and toxicological trials, thus requiring the application of appropriate extraction techniques for the detection of these antihypertensive substances at nanogram and picogram levels.

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“…Literature survey revealed HPLC [1] , LC-MS [2] , spectroflurimetric and simultaneous UVspectrophotometric methods are reported for the estimation of aliskiren hemifumarate [3][4] , amlodipine [5][6][7][8][9][10] , hydrochlorothiazide [11][12][13][14][15] alone or in combination with other anti-hypertensive agents. So the present study aim to develop a simple, selective and precise RP-HPLC method was developed for the simultaneous estimation of Aliskiren hemifumarate, amlodipine besylate and hydrochlorothiazide in bulk drug and in combined dosage form.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Stability Indicating RP-HPLC Method for Simultaneous Estimation of Aliskiren Hemifumarate, Amlodipine Besylate and Hydrochlorothiazide in Bulk and Pharmaceutical Dosage Forms

Prathyusha¹,

Tengli²,

Chandan³

et al. 2014

IOSRJPBS

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Simultaneous quantitation of hydrochlorothiazide and metoprolol in human plasma by liquid chromatography–tandem mass spectrometry

Cited by 42 publications

References 13 publications

Total error-based validation including the experimental design-based robustness evaluation of a stability-indicating method for the simultaneous quantification of hydrochlorothiazide and valsartan in tablet formulations

Total error-based validation including the experimental design-based robustness evaluation of a stability-indicating method for the simultaneous quantification of hydrochlorothiazide and valsartan in tablet formulations

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Development and Validation of a Stability Indicating RP-HPLC Method for Simultaneous Estimation of Aliskiren Hemifumarate, Amlodipine Besylate and Hydrochlorothiazide in Bulk and Pharmaceutical Dosage Forms

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