Simultaneous quantitative determination of multiple analytes with fluorescence‐tagged probes by Immunochromatography

Jeong, Dong Seok; Choi, Eui Yul

doi:10.1080/12265071.2003.9647688

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Immunochromatography can quickly determine the analytes without separating the combined marker and the free marker, so it is suitable for on-site test [2][3] .…”

Section: Introductionmentioning

confidence: 99%

Design of time-resolved fluorometer based on immunochromatography

Ren

Huang

2008

SPIE Proceedings

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This paper introduces the design of a novel time-resolved fluorometer based on immunochromatograghy. Different from the other time-resolved fluorometers, it tests the immunochromatographic strip which is labeled with lanthanide ions and their chelates. This instrument can provide a rapid, quantitative measurement of analytes present in samples without any washing steps and it can be used to carry out point-of-care test (POCT). The immunochromatograghy-based timeresolved fluorometer is composed of a specific optical sensor, a scanning stage, a signal processing system and a computer control system. The light from UV LED is focused on the test strip by a condense lens group in the optical sensor. If the labels are present in samples, the fluorescence at 613nm will be exited (when Eu 3 + chelate is used for marking substance). After a delay of some microseconds, the fluorescence will be collected by the optical sensor and converted into electronic signal by a photomultiplier tube (PMT). The concentration of the sample can be calculated through the standard working curve of this instrument. By testing, the sensitivity is several ng/ml level (when Eu 3 + chelate is used for marking substance), test linear range is from several ng/ml to 10 3 ng/ml, in which correlation coefficient is 99.97%.

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“…Immunochromatography can quickly determine the analytes without separating the combined marker and the free marker, so it is suitable for on-site test [2][3] .…”

Section: Introductionmentioning

confidence: 99%

Design of time-resolved fluorometer based on immunochromatography

Ren

Huang

2008

SPIE Proceedings

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“…The fluorescence immune chromatography test technology combines the specificity of the antigen-antibody reaction and the sensitivity of the fluorescence technique. It is widely recognized as the most sensitive detecting technology, and is widely used in the clinical detection like human chorionic gonadotropin (HCG), plague, hepatitis B, parasites, AIDS [1][2].…”

Section: Introductionmentioning

confidence: 99%

Research of Quantitative Analyzer for Fluorescence Immune Chromatography Strips

Jiang

Wang

2013

AMM

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Fluorescence immunoassay technology is widely recognized as the most sensitive detection technology, now widely used in clinical testing. The research of quantitative analyzer for fluorescence immune chromatography strips is of great significance. In this thesis, a quantitative analyzer for fluorescence immune chromatography strips has realized, based on photoelectric detection principle and immunochromatographic. The system uses LPC1788 and MSP430 as processors. Using some algorithms such as low-pass filtering, least square method and area method to analyze the detected waveform and eliminate noise with high accuracy. In our experiment, the minimum detection concentration is at several mIU/ml levels, the relative standard deviation is less than 2% and it has good linear response characteristics in a concentration range from 0.5125 to 952mIU/ml while the correlation coefficient R2 is 0.981. The instrument has a good prospect.

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