Simultaneous real-time amperometric measurement of catecholamines and serotonin at carbon fibre ‘dident’ microelectrodes

Pennington, James; Millar, Jane; Jones, Clinton P. L.; Owesson, Catarina Å.; McLaughlin, Daniel P.; Stamford, Jonathan A.

doi:10.1016/j.jneumeth.2004.03.023

Cited by 16 publications

(9 citation statements)

References 28 publications

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“…Biological electrochemistry applications include amperometric detection of oxidizable neurotransmitter Chow 1996, 1998) or hormone secretion (Zhang et al 1999), as well as detection of neurotransmitters in vivo by cyclic voltammetry (Clark et al 2010;Robinson et al 2003). Carbon fiber microelectrodes with multiple sensing elements have also been developed to carry out electrochemical experiments with high spatial resolution (Dressman et al 2002;Zhang et al 2008) or to detect multiple neurotransmitters simultaneously (Pennington et al 2004). In addition, multichannel iontophoresis barrels have been combined with a single low-impedance carbon fiber recording tip (Armstrong-James and Millar 1979;Budai and Molnár 2001;Fu and Lorden 1996;Inagaki et al 2009).…”

Section: Resultsmentioning

confidence: 99%

Ultrasmall and customizable multichannel electrodes for extracellular recordings

Piironen

Weckström

Vähäsöyrinki

2011

Journal of Neurophysiology

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Increasing demand exists for smaller multichannel electrodes that enable simultaneous recordings of many neurons in a noninvasive manner. We report a novel method for manufacturing ultrasmall carbon fiber electrodes with up to seven closely spaced recording sites. The electrodes were designed to minimize damage to neuronal circuitry and to be fully customizable in three dimensions so that their dimensions can be optimally matched to those of the targeted neuron population.

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Section: Resultsmentioning

confidence: 99%

Ultrasmall and customizable multichannel electrodes for extracellular recordings

Piironen

Weckström

Vähäsöyrinki

2011

Journal of Neurophysiology

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“…The exposed carbon fiber was trimmed to a final length of ~100 μm using a scalpel 10. To exclude negatively charged interference, the tip of the CFM was dip coated with 5 % Nafion and dried under a heat gun three times 45. The Ag/AgCl reference electrode was fabricated by chloridizing a 31 gauge silver wire 16.…”

Section: Methodsmentioning

confidence: 99%

Wireless Instantaneous Neurotransmitter Concentration System: electrochemical monitoring of serotonin using fast-scan cyclic voltammetry—a proof-of-principle study

Griessenauer¹,

Su²,

Tye³

et al. 2010

JNS

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Object-We previously reported the development of a Wireless Instantaneous Neurotransmitter Concentration System (WINCS) for measuring dopamine and suggested that this technology may be useful for evaluating deep brain stimulation (DBS)-related neuromodulatory effects on neurotransmitter systems. WINCS supports fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) for real-time, spatially resolved neurotransmitter measurements. The FSCV parameters used to establish WINCS dopamine measurements are not suitable for serotonin, a neurotransmitter implicated in depression, because they lead to CFM fouling and a loss of sensitivity. Here, we incorporate into WINCS a previously described N-shaped waveform applied at a high scan rate to establish wireless serotonin monitoring.Methods-FSCV optimized for the detection of serotonin consisted of an N-shaped waveform scanned linearly from a resting potential of, in V, +0.2 to +1.0, then to −0.1 and back to +0.2 at a rate of 1000 V/s. Proof of principle tests included flow injection analysis and electrically evoked serotonin release in the dorsal raphe nucleus of rat brain slices.Results-Flow cell injection analysis demonstrated that the N waveform applied at a scan rate of 1000 V/s significantly reduced serotonin fouling of the CFM, relative to that observed with FSCV parameters for dopamine. In brain slices, WINCS reliably detected sub-second serotonin release in the dorsal raphe nucleus evoked by local high-frequency stimulation.Conclusion-WINCS supported high-fidelity wireless serotonin monitoring by FSCV at a CFM. In the future such measurements of serotonin in large animal models and in humans may help to establish the mechanism of DBS for psychiatric disease.

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“…In separate solutions of 0.1 M phosphate buffered saline (pH 7.4, warmed to 37°C), the microelectrode arrays were challenged with infusions of dopamine (DA − 20 nM to 50 µM), ascorbic acid (AA − 1 to 200 µM), dihydroxyphenylacetic acid (DOPAC − 1 to 200 µM), and homovanillic acid (HVA − 1 to 200 µM). These analytes were detected through constant potential amperometry at +500 mV in reference to an Ag/AgCl glass body electrode (Kawagoe and Wightman, 1994; Pennington et al, 2004). Devices typically achieved selectivity ratios greater than 100:1 for AA, DOPAC, and HVA and DA detection limits of 200 nM post-implantation, which were defined as current steps with a signal-to-noise ratio greater than three.…”

Section: Methodsmentioning

confidence: 99%

“…Here, we describe an intracranial, silicon-substrate probe that facilitates concurrent recordings of LFPs and spikes on one set of microelectrodes (Blanche et al, 2005) and detection of extra-synaptic dopamine dynamics on an adjacent set of microelectrodes through constant potential amperometry (Kawagoe and Wightman, 1994; Pennington et al, 2004). These multi-modal probes were implanted in the striatum of urethane-anesthetized rats to examine 1) reciprocal influences of amperometry on neuronal activity, and 2) spatiotemporal characteristics of dopamine efflux and LFP/spike activity following electrical stimulation of the medial forebrain bundle (MFB).…”

Section: Introductionmentioning

confidence: 99%

Implantable microelectrode arrays for simultaneous electrophysiological and neurochemical recordings

Johnson¹,

Franklin²,

Gibson³

et al. 2008

Journal of Neuroscience Methods

101

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Implantable microfabricated microelectrode arrays represent a versatile and powerful tool to record electrophysiological activity across multiple spatial locations in the brain. Spikes and field potentials, however, correspond to only a fraction of the physiological information available at the neural interface. In urethane-anesthetized rats, microfabricated microelectrode arrays were implanted acutely for simultaneous recording of striatal local field potentials, spikes, and electrically-evoked dopamine overflow on the same spatiotemporal scale. During these multi-modal recordings we observed 1) that the amperometric method used to detect dopamine did not significantly influence electrophysiological activity, 2) that electrical stimulation in the medial forebrain bundle (MFB) region resulted in electrochemically-transduced dopamine transients in the striatum that were spatially heterogeneous within at least 200 µm, and 3) following MFB stimulation, dopamine levels and electrophysiological activity within the striatum exhibited similar temporal profiles. These neural probes are capable of incorporating customized microelectrode geometries and configurations, which may be useful for examining specific spatiotemporal relationships between electrical and chemical signaling in the brain.

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Simultaneous real-time amperometric measurement of catecholamines and serotonin at carbon fibre ‘dident’ microelectrodes

Cited by 16 publications

References 28 publications

Ultrasmall and customizable multichannel electrodes for extracellular recordings

Ultrasmall and customizable multichannel electrodes for extracellular recordings

Wireless Instantaneous Neurotransmitter Concentration System: electrochemical monitoring of serotonin using fast-scan cyclic voltammetry—a proof-of-principle study

Implantable microelectrode arrays for simultaneous electrophysiological and neurochemical recordings

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