Simultaneous recording of local electrical activity, partial oxygen tension and temperature in the rat hippocampus with a chamber-type microelectrode. Effects of anaesthesia, ischemia and epilepsy

Energy deprivation transiently enhances rhythmic inhibitory events in the CA3 hippocampal network in vitro

“…Thus, even though neurons initially hyperpolarize in response to energy deprivation (Krnjević, 2008), we propose that a sufficient number of interneurons are transiently activated through glutamate-induced transmitter release to generate a transient phase of oscillatory activity. Previous studies in vivo (Freund et al, 1989) and in the whole hippocampus of neonatal rats (Dzhala et al, 2001) reported that energy deprivation induced a transient burst of gamma oscillations, whereas in our study in slice cultures we observed oscillations at lower frequencies close to the theta range. As pointed out by Dzhala and colleagues (2001), the neonatal tissue they used may not support theta oscillations, which arise after day 8 in rodents (Leblanc and Bland, 1979;Leinekugel et al;2002, but see Karlsson and Blumberg, 2003).…”

“…Changes in membrane properties and in synaptic function are expected also to modify network activity in neuronal populations. Indeed, ischemia induced an epoch of high frequency discharge recorded from the hippocampus in anesthetized rats just prior to the suppression of synaptic activity (Freund et al, 1989). And in an in vitro preparation consisting of the whole hippocampus from neonatal rats, anoxia induced gamma oscillations originating in the CA3 area (Dzhala et al, 2001).…”

Hypertonic Saline Treatment of Acid Aspiration-Induced Lung Injury

“…Miniaturized thin-film oxygen sensor (Otto Sensors Co., Cleveland, OH) was used. This new chamber-type microelectrode has been described in detail elsewhere [18,19]. In brief, the oxygen sensor consists of a Au working electrode, 20 mm in diameter, and two Ag/AgCl reference electrodes, each measuring 1800 mm 2 .…”

Simultaneous recording of hippocampal oxygen and glucose in real time using constant potential amperometry in the freely-moving rat

“…However, there has been less attention has been afforded to the hippocampus (Freund et al, 1989;Hu and Wilson, 1997) despite its pivotal roles in declarative memory (Scoville and Milner, 1957;Cohen et al, 1999;Eacott andEaston, 2011), spatial navigation (O'Keefe andNadel, 1978;Morris et al, 1982;D'Hooge and De Deyn, 2001) and its links to various neurodegenerative/psychiatric disorders (Heckers and Konradi, 2010;Marlatt and Lucassen, 2010;Bast, 2011;Dhikav and Anand, 2011;Bonilha et al, 2012). Therefore, there is a need for sensor data recorded in real-time to supplement the large amount of electrophysiological data (Bliss and Lomo, 1973;Martin et al, 2000;Morris et al, 2003;Colgin and Moser, 2010), microdialysis data (McNay et al, 2001;Gold, 2003;De Bundel et al, 2009;López-Pérez et al, 2012) and molecular data from tissue samples (Gooney et al, 2002;Minichiello, 2009;Barry and Commins, 2011) already published from work in the hippocampus.…”