Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometry

Zhang, Wei-Ting; Chao, Tzu-Hao Harry; Cui, Guohong; Shih, Yu Jen

doi:10.1016/j.xpro.2022.101497

Cited by 7 publications

(17 citation statements)

References 7 publications

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“…We used an fMRI-compatible, spectrally resolved, fiber-photometry platform (Bao et al, 2020; Chao et al, 2022; Das et al, 2021; Meng et al, 2018; Oyarzabal et al, 2022; Zhang et al, 2022b, 2022a) with four recording fibers ( Figure 1A ) to measure neuronal GCAMP activity in the RSC, Cg, PrL, and AI from rats ( Supplementary Figure 1 ). GCaMP activity was combined with whole-brain fMRI recordings in anesthetized rodents to capture concurrent resting-state signals from both modalities ( Figure 1A ).…”

Section: Resultsmentioning

confidence: 99%

“…A MRI-compatible, four-channel, spectrally resolved, fiber-photometry platform was used (Bao et al, 2020; Chao et al, 2022; Das et al, 2021; Meng et al, 2018; Oyarzabal et al, 2022; Zhang et al, 2022b, 2022a). We used interleaved 488 nm (OBIS Galaxy Laser 1236444, Coherent, Santa Clara, CA) and 405 nm (OBIS Galaxy Laser 1236439, Coherent, Santa Clara, CA) diode lasers for GCaMP signal excitation.…”

Section: Methodsmentioning

confidence: 99%

“…; ANTISEDAN, Orion, Espoo, Finland) for the reversal of the sedative and analgesic effects of dexmedetomidine, and sugammadex sodium (4 to 8mg/kg, i.v. ; Merck Sharp & Dohme Corp., Kenilworth, New Jersey) for the reversal of the paralytic effect of rocuronium (Chao et al, 2018, 2022, Zhang et al, 2022b, 2022a).…”

Section: Methodsmentioning

confidence: 99%

“…Here, to form a comprehensive understanding of neuronal signaling among putative rodent DMN and SN areas in a behaviorally relevant context and build an accurate and translational model of the DMN and its dynamic properties, we concurrently measured neuronal calcium activity in the RSC, Cg, PrL, and AI of Thy1-GCaMP6f transgenic rats (Scott et al, 2018). To enable these measurements, we developed an fMRI-compatible, four-channel, spectrally resolved, fiber-photometry recording system based on the platform used in our previous studies (Bao et al, 2020; Chao et al, 2022; Das et al, 2021; Meng et al, 2018; Oyarzabal et al, 2022; Zhang et al, 2022b, 2022a). We used our novel fiber-photometry platform and recent advances in computational modeling of brain-circuit dynamics to characterize neuronal dynamics of the putative DMN nodes and AI under resting-state and awake, salient stimulus presentation conditions.…”

Section: Introductionmentioning

confidence: 99%

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Neuronal dynamics of the default mode network and anterior insular cortex: Intrinsic properties and modulation by salient stimuli

Chao

Lee

Hsu

et al. 2022

Preprint

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The default mode network (DMN) is closely associated with self-referential mental functions and its dysfunction is implicated in many neuropsychiatric disorders. However, the neurophysiological properties and task-based functional organization of the rodent DMN are poorly understood, limiting its translational utility. Here, we combine fiber-photometry with fMRI and computational modeling to characterize dynamics of putative rodent DMN nodes and their interactions with the anterior insular cortex (AI) of the salience network. We reveal neuronal activity changes in AI and DMN nodes prior to fMRI-derived DMN activations and uncover cyclical transition patterns between spatiotemporal neuronal activity states. Finally, we demonstrate that salient oddball stimuli suppress the DMN and enhance AI neuronal activity, and that the AI causally inhibits the retrosplenial cortex, a prominent DMN node. These findings elucidate previously unknown properties regarding the neurobiological foundations of the rodent DMN and its modulation by salient stimuli, paving the way for future translational studies.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Neuronal dynamics of the default mode network and anterior insular cortex: Intrinsic properties and modulation by salient stimuli

Chao

Lee

Hsu

et al. 2022

Preprint

Self Cite

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“…We present use of this technique to remove Hb absorption artifacts from calcium activity recording and demonstrate the added benefit to compute the transfer function between neuronal and vascular activity, which is crucial for interpreting hemodynamics-based neuroimaging data such as fMRI, functional ultrasound, and intrinsic optics. A step-by-step protocol of this method could be found in Zhang et al (2022) .…”

Section: Introductionmentioning

confidence: 99%

Spectral fiber photometry derives hemoglobin concentration changes for accurate measurement of fluorescent sensor activity

Zhang

Chao

Yang

et al. 2022

Cell Reports Methods

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Distinct neurochemical influences on fMRI response polarity in the striatum

Cerri,

Albaugh,

Walton

et al. 2024

Nat Commun

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The striatum, known as the input nucleus of the basal ganglia, is extensively studied for its diverse behavioral roles. However, the relationship between its neuronal and vascular activity, vital for interpreting functional magnetic resonance imaging (fMRI) signals, has not received comprehensive examination within the striatum. Here, we demonstrate that optogenetic stimulation of dorsal striatal neurons or their afferents from various cortical and subcortical regions induces negative striatal fMRI responses in rats, manifesting as vasoconstriction. These responses occur even with heightened striatal neuronal activity, confirmed by electrophysiology and fiber-photometry. In parallel, midbrain dopaminergic neuron optogenetic modulation, coupled with electrochemical measurements, establishes a link between striatal vasodilation and dopamine release. Intriguingly, in vivo intra-striatal pharmacological manipulations during optogenetic stimulation highlight a critical role of opioidergic signaling in generating striatal vasoconstriction. This observation is substantiated by detecting striatal vasoconstriction in brain slices after synthetic opioid application. In humans, manipulations aimed at increasing striatal neuronal activity likewise elicit negative striatal fMRI responses. Our results emphasize the necessity of considering vasoactive neurotransmission alongside neuronal activity when interpreting fMRI signal.

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Simultaneous recording of neuronal and vascular activity in the rodent brain using fiber-photometry

Cited by 7 publications

References 7 publications

Neuronal dynamics of the default mode network and anterior insular cortex: Intrinsic properties and modulation by salient stimuli

Neuronal dynamics of the default mode network and anterior insular cortex: Intrinsic properties and modulation by salient stimuli

Spectral fiber photometry derives hemoglobin concentration changes for accurate measurement of fluorescent sensor activity

Distinct neurochemical influences on fMRI response polarity in the striatum

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