Simultaneous regulation of optical properties and cellular behaviors of gold nanoclusters by pre-engineering the biotemplates

Abstract

Herein, we reported a new strategy to simultaneously regulate both physicochemical properties and biological behaviors of fabricated nanomaterials. Upon precisely pre-tailoring the amounts of charged groups of bovine serum albumin...

Supercharged Protein Nanosheets for Cell Expansion on Bioemulsions

“…These observations raise the possibility of engineering protein nanosheets via the design of their amino acid composition and chemistry. To further demonstrate this concept, we studied the impact of functionalization of BSA with succinic anhydride (leading to a negatively supercharged protein, aBSA, with a ζ-potential of −31.4 mV) and ethylene diamine residues (leading to a positively supercharged protein, cBSA, with a ζ-potential of +13.9 mV) [21] on self-assembly and interfacial mechanics (Figure 2). In the absence of co-surfactant, the surface chemistry of BSA had a striking impact on the mechanical properties of corresponding nanosheets assembled at Novec 7500-water interfaces.…”

“…Materials and Chemicals. Native, anionic and cationic BSA were prepared and provided as described in [21] . The fluorinated surfactant 2,3,4,5,6,-Perfluorobenzoyl chloride, PBS, Trichloro (1H,1H,2H,2Hperfluorooctyl) silane (97%) and the 1H,1H,2H,2H-Perfluorodecanethiol (97%) were purchased from Sigma Aldrich Co.…”

“…Chemical modifications of proteins can lead to significant structural changes and can help in understanding the role of electrostatic interactions on their stability. For example, modifications such as succinylation and acetylation can expose, or block, reactive amino acids in a protein, affecting protein-protein interactions, normal unfolding and aggregation [18] [19] [20][21] . The extent to which the structure of the protein is affected by such external factors is often determined by the natural rigidity of the protein.…”

Metal–Organic Frameworks-Mediated Assembly of Gold Nanoclusters for Sensing Applications

“…Significantly, characteristics of AuNCs, such as emission colors, surface ligands, chemical compositions and core sizes, can also be regulated facilely via various synthesis strategies. On account of the advantages mentioned above, AuNCs have been widely applied in various fields, including catalysis [ 25 , 27 ], bioimaging [ 28 , 29 ], sensing [ 30 , 31 ], anticancer therapy [ 32 ], and antisepsis [ 33 – 35 ]. Particularly, in sensing fields, AuNCs have been intensively exploited as novel probes for the sensing of a wide range of different analytes, such as metal ions [ 36 , 37 ], biomolecules [ 38 , 39 ], temperature [ 40 ], small molecules [ 41 ], bacteria [ 42 ], cancer cells [ 43 ], and so on.…”

Synthesis of Vitamin B1-Stabilized Gold Nanoclusters with High Quantum Yields for Application as Sensors

“…Furthermore, different kinds of strategies have also been developed to enhance the QY of AuNCs, such as changing the protecting ligands, modulating the synthetic approaches, doping them with other atoms, ligand exchange, controllable oxidation, and so forth. − These methods have been proven to effectively enhance the QY of AuNCs. For example, Aldeek and co-workers proposed a ligand exchange methodology, which can result in a 4-fold enhancement QY of AuNCs from 3.5% to ca.…”