Simultaneous Saccharification and Fermentation of Aqueous Ammonia Pretreated Oat Straw for Ethanol Production

Karki, Bishnu; Rijal, Binod; Pryor, Scott W.

doi:10.13031/2013.39816

Cited by 8 publications

(2 citation statements)

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“…Enzyme loadings have been reported as the most important factor after effective pretreatment for increasing the rate or yield of enzymatic hydrolysis [26,27]. Our models show that glucose and xylose are similarly sensitive to cellulase and hemicellulase for SAA pretreated corn stover.…”

Section: Figurementioning

confidence: 69%

Process yield and economic trade-offs for enzymatic hydrolysis of alkaline pretreated corn stover

Nahar

Ripplinger

Pryor

2017

Biomass and Bioenergy

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Response surface methodology was used to investigate the interaction of pH, temperature, and enzyme loadings on corn stover hydrolysis rates following soaking in aqueous ammonia pretreatment. Economic tradeoffs were estimated for cellulase and hemicellulase loadings under different hydrolysis conditions. Enzyme loadings had a more significant effect on rates than did pH or temperature. The effect of hydrolysis pH was independent of temperature and enzyme loadings, and the optimal pH for glucose and xylose yields were 4.5 and 4.3, respectively. Conducting hydrolysis at 50 °C rather than 37 °C enables either a 10% glucose yield increase, or a comparable yield with 40% and 65% reduction in cellulase and hemicellulase loadings, respectively. Although yield models showed that hydrolysis rates increase with higher enzyme loadings, economic models showed that optimal cellulase and hemicellulase loadings were as much as 47% and 23% lower, respectively, than the maximum loadings tested. Optimal enzyme loadings change with fluctuations in enzyme costs and ethanol price, but cellulase loadings were more sensitive to these changes than hemicellulase loadings. Enzyme loadings were also more sensitive to enzyme price at lower processing temperatures. Enzyme loadings can be adjusted to increase return based on enzyme costs, ethanol price, and process temperature.

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Section: Figurementioning

confidence: 69%

Process yield and economic trade-offs for enzymatic hydrolysis of alkaline pretreated corn stover

Nahar

Ripplinger

Pryor

2017

Biomass and Bioenergy

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“…Oat straw and husks were investigated for the production of biogas [10], xylanase [11], xylitol [12], ethanol [12,13], bacterial cellulose [14][15][16], hydrogels [17], and polypropylene composites [18]. The pretreatment strategies to overcome lignocellulosic recalcitrance mostly included dilute-acid pretreatment using sulfuric acid [11,19] and nitric acid [13,[20][21][22][23], which exhibited the highest impact on dissolution of hemicellulose, and dilute-alkali pretreatment using sodium hydroxide [17,24] and ammonium hydroxide [25], which showed the highest impact on delignification. The use of concentrated salts solutions such as sodium benzoate [26] were also investigated for delignification.…”

Section: Introductionmentioning

confidence: 99%

Biorefining Oat Husks into High-Quality Lignin and Enzymatically Digestible Cellulose with Acid-Catalyzed Ethanol Organosolv Pretreatment

2020

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Oat husks are low-value lignocellulosic residues of oat processing that carry an environmental impact. Their polymers (cellulose, hemicellulose, and lignin) can be converted into a wide variety of value-added products; however, efficient pretreatment methods are needed that allow their fine separation for further tailored valorization. This study pioneered the use of milling-free and low acid-catalyzed ethanol organosolv for the delignification of oat husks, allowing their conversion into three high-quality streams, namely, glucan-rich, lignin-rich, and hemicellulosic compound-rich streams. Temperature, retention time, and solid-to-liquid ratio were found to impact the delignification of oat husks when using a one-factor-at-a-time strategy. The ideal conditions that were found (210 °C, 90 min, and solid-to-liquid ratio of 1:2) culminated into glucan and lignin fractions containing 74.5% ± 11.4% glucan and 74.9% ± 7.6% lignin, respectively. These high-purity lignin fractions open the possibility for higher value applications by lignin, potentially impacting the feasibility of second generation biorefineries. The glucan fraction showed 90% digestibility after 48 h of hydrolysis with 10 filter paper units of enzyme cocktail per gram of glucan. Considering the absence of size reduction and high solid loading, together with the quality of the obtained streams, organosolv pretreatment could be a potential strategy for the valorization of oat lignocellulosic residues.

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Pretreatment of Lignocellulosic Biomass

Amarasekara¹

2013

Handbook of Cellulosic Ethanol

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Simultaneous Saccharification and Fermentation of Aqueous Ammonia Pretreated Oat Straw for Ethanol Production

Cited by 8 publications

References 0 publications

Process yield and economic trade-offs for enzymatic hydrolysis of alkaline pretreated corn stover

Process yield and economic trade-offs for enzymatic hydrolysis of alkaline pretreated corn stover

Biorefining Oat Husks into High-Quality Lignin and Enzymatically Digestible Cellulose with Acid-Catalyzed Ethanol Organosolv Pretreatment

Pretreatment of Lignocellulosic Biomass

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