Simultaneous saccharification and fermentation of corncobs with genetically modified<i>Saccharomyces cerevisiae</i>and characterization of their microstructure during hydrolysis

Song, Huiting; Liu, Shihui; Gao, Yuan; Yang, Yimin; Xiao, Wen‐Jing; Xia, Wu-Cheng; Liu, Zilu; Li, Rong; Ma, Xinrong; Jiang, Zhengbing

doi:10.1080/21655979.2016.1178424

Cited by 9 publications

(8 citation statements)

References 18 publications

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“…Synthetic complete minimal medium without uracil (SC-U) medium (1.34% yeast nitrogen base, 2% glucose, 0.01% histidine, 0.01% leucine, 0.01% tryptophan, and 2% agar) was used to screened transformants. An rDNA-mediated integrated expression vector, named pHBM368-pgk, was used for the expression of target genes in S. cerevisiae [ 28 ]. Three cellulase genes, eg (GenBank: CAL48345), cbh (GenBank: AGT15838) and bg (GenBank: ADY18331), were chemically synthesized (Generay Biotech Co., Ltd., Shanghai), then cloned into pHBM368-pgk, to produce the recombinant plasmids named pHBM368-pgk-eg, pHBM368-pgk-cbh and pHBM368-pgk-bg, respectively.…”

Section: Methodsmentioning

confidence: 99%

Construction of a trifunctional cellulase and expression in Saccharomyces cerevisiae using a fusion protein

Liu

Song

et al. 2018

BMC Biotechnol

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BackgroundCellulose is the most important component of lignocellulose, and its degradation requires three different types of enzymes to act synergistically. There have been reports of single gene duality, but no gene has been described to have more than two functions. Cloning and expression of fusion cellulases containing more than two kinds of catalytic domains has not been reported thus far.ResultsWe synthesized three different cellulase genes and linked the three catalytic domains with a (G4S)3 flexible linker. The trifunctional cellulase gene (BCE) containing three types of cellulase functions was constructed and expressed in S. cerevisiae successfully. The β-glucosidase, the exoglucanase and the endoglucanase activity of the trifunctional cellulase BCE reached 16.80 IU/mg, 2.26 IU/mg and 20.67 IU/mg, which was 46.27, 6.73 and 46.20% higher than the activities of the β-glucosidase BG, the endoglucanase CBH and the endoglucanase EG. The filter paper enzyme activity of BCE was higher than those of BG, CBH and EG, reached 2.04 IU/mg.ConclusionsThe trifunctional cellulase BCE was designed based on β-glucosidase BG, endoglucanase EG and exoglucanase CBH, and it possessed β-glucosidase activity, endoglucanase activity and exoglucanase activity simultaneously. The BCE has better filter paper activity, it means the potential practical application.Electronic supplementary materialThe online version of this article (10.1186/s12896-018-0454-x) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Construction of a trifunctional cellulase and expression in Saccharomyces cerevisiae using a fusion protein

Liu

Song

et al. 2018

BMC Biotechnol

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“…In the bioethanol industry, different yeast strains are used for production in Brazil, where the feedstock is sugar cane (Basso et al 2008), and in the USA, where the feedstock is primarily corn (Song et al 2016). Thus, for designing a new bioprocess, it is important to have in mind what the feedstock is going to be for the final bioprocess.…”

Section: Challenges In Developing New Bioprocessesmentioning

confidence: 99%

“…Whereas the major challenge in developing a new bioprocess is with developing the cell factory, there are also challenges associated with matching the cell factory design with the feedstock that is going to be used for the production. In the bioethanol industry, different yeast strains are used for production in Brazil, where the feedstock is sugar cane (Basso et al 2008 ), and in the USA, where the feedstock is primarily corn (Song et al 2016 ). Thus, for designing a new bioprocess, it is important to have in mind what the feedstock is going to be for the final bioprocess.…”

Section: Challenges In Developing New Bioprocessesmentioning

confidence: 99%

Building a bio-based industry in the Middle East through harnessing the potential of the Red Sea biodiversity

Nielsen

Archer

Essack

et al. 2017

Appl Microbiol Biotechnol

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The incentive for developing microbial cell factories for production of fuels and chemicals comes from the ability of microbes to deliver these valuable compounds at a reduced cost and with a smaller environmental impact compared to the analogous chemical synthesis. Another crucial advantage of microbes is their great biological diversity, which offers a much larger “catalog” of molecules than the one obtainable by chemical synthesis. Adaptation to different environments is one of the important drives behind microbial diversity. We argue that the Red Sea, which is a rather unique marine niche, represents a remarkable source of biodiversity that can be geared towards economical and sustainable bioproduction processes in the local area and can be competitive in the international bio-based economy. Recent bioprospecting studies, conducted by the King Abdullah University of Science and Technology, have established important leads on the Red Sea biological potential, with newly isolated strains of Bacilli and Cyanobacteria. We argue that these two groups of local organisms are currently most promising in terms of developing cell factories, due to their ability to operate in saline conditions, thus reducing the cost of desalination and sterilization. The ability of Cyanobacteria to perform photosynthesis can be fully exploited in this particular environment with one of the highest levels of irradiation on the planet. We highlight the importance of new experimental and in silico methodologies needed to overcome the hurdles of developing efficient cell factories from the Red Sea isolates.

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“…In a previous study, the S. cerevisiae strains expressing three different kinds of cellulase (CBH/EG/BG) were constructed respectively, and these recombinant strains were used to hydrolyze the corncob during SSF (Song et al 2016). Based on previous research in the authors' lab, surfactants with potential for development in SSF by using genetically modified S. cerevisiae to produce bio-ethanol from corncob were investigated.…”

Section: Introductionmentioning

confidence: 99%

Effect of different biological surfactants on engineering Saccharomyces cerevisiae in simultaneous saccharification and fermentation of corncob

Xiao

Song

et al. 2020

BioRes

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Lignocellulose is considered to be a good resource for producing renewable energy. This paper reports on the effect of three surfactants [polyoxyethylene (80) sorbitan monooleate (POE80), rhamnolipid, and tea saponin] on cellulase (CBH/EG/BG) expression of Saccharomyces cerevisiae in simultaneous saccharification and fermentation (SSF) of corncob. In this work, the optimal surfactant concentrations for yeast growth were 0.1% POE80, 0.05% rhamnolipid, and 0.002% tea saponin. In the process of SSF, the reducing sugar content with 0.1% POE80 was 13.5% higher than the control at 24 h. The reducing sugar content with 0.05% rhamnolipid was higher than the control at 120 h, and reached the maximum difference of 18.2% in 120 h. The addition of 0.002% tea saponin exhibited the lowest promotion effect on the reducing sugar content in SSF compared with POE80 and rhamnolipid. However it reached the maximum difference of 8% in 120 h. Compared with the control, 0.1% POE80, 0.05% rhamnolipid, and 0.002% tea saponin presented different degrees of increase in reducing sugar content and viable count in the SSF. The results showed that the addition of the surfactants in SSF increased the growth rate of strains and promoted the saccharification efficiency of the substrate. This study lays a foundation for the application of surfactants in bio-energy research.

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Simultaneous saccharification and fermentation of corncobs with genetically modifiedSaccharomyces cerevisiaeand characterization of their microstructure during hydrolysis

Cited by 9 publications

References 18 publications

Construction of a trifunctional cellulase and expression in Saccharomyces cerevisiae using a fusion protein

Construction of a trifunctional cellulase and expression in Saccharomyces cerevisiae using a fusion protein

Building a bio-based industry in the Middle East through harnessing the potential of the Red Sea biodiversity

Effect of different biological surfactants on engineering Saccharomyces cerevisiae in simultaneous saccharification and fermentation of corncob

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