Simultaneous separate and group determination of tylosin and tilmicosin in foodstuffs using single antibody-based immunoassay

Burkin, Maksim A.; Galvidis, Inna A.

doi:10.1016/j.foodchem.2011.11.029

Cited by 29 publications

(13 citation statements)

References 19 publications

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“…Besides, the equivalence and/or similarity in cross-reactivity of the 14-membered macrolides ERY, CLA, and ROX allowed their quantification using one marker analyte. The related approach was described previously for the tylosin/tilmicosin model [24]. Note.…”

Section: Discussionmentioning

confidence: 99%

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Group determination of 14-membered macrolide antibiotics and azithromycin using antibodies against common epitopes

Galvidis

Lapa

Burkin

2015

Analytical Biochemistry

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Section: Discussionmentioning

confidence: 99%

“…Pretreatment of honey was carried out according to the procedure described in our previous work concerning 16-membered macrolides [24]. Briefly, honey samples melted at 50°C were taken in a 1-cm 3 syringe without a needle and then added to PBS-T and dissolved.…”

Section: Sample Preparationmentioning

confidence: 99%

Group determination of 14-membered macrolide antibiotics and azithromycin using antibodies against common epitopes

Galvidis

Lapa

Burkin

2015

Analytical Biochemistry

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“…Nos Estados Unidos, o uso da tilosina é aprovado tanto para fins terapêuticos como promotor de crescimento em animais. Em geral, no mundo inteiro as drogas mais utilizadas como promotores de crescimento incluem os macrolídeos (tilosina e espiramicina) dentre outras classes de antimicrobianos [16,17] . A Organização Mundial da Saúde (OMS) classificou os antibióticos macrolídeos como uma das três principais classes para o desenvolvimento de estratégias de gestão de risco a fim de preservar sua eficácia na medicina humana, já que a resistência pode emergir em patógenos zoonóticos como Campylobacter spp.…”

Section: Introductionunclassified

“…-Desenvolvimento de micro-organismos resitentes a antimicrobianos usados na terapia humana [19,16] .…”

Section: Introductionunclassified

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Tilosina: um importante antibiótico não monitorado em leite no Brasil

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Spisso

Pereira

et al. 2015

Segur. Aliment. Nutr.

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A introdução de agentes antimicrobianos na alimentação animal revolucionou a produção de alimentos no mundo, porém o uso indiscriminado desses agentes podem provocar agravos à saúde da população. Os macrolídeos constituem a segunda classe de antimicrobianos mais usados nos animais em geral, sendo a tilosina presente em um maior número de formulações disponíveis para bovinos. A contaminação do leite por resíduos de tilosina acima dos valores considerados seguros pode constituir um risco potencial à saúde do consumidor. Com o objetivo de obter uma visão geral da atual situação da segurança de alimentos em relação a este problema, este estudo foi realizado mediante levantamento bibliográfico na base de dados SciFinder Scholar e periódicos CAPES. Foi constatado que no Brasil existem dois programas em andamento para avaliar a presença de resíduos de medicamentos veterinários em alimentos: PNCRC e PAMVet. Entretanto, a tilosina não consta no escopo analítico destes programas. Dos artigos que tratam da contaminação do leite por resíduos de tilosina publicados entre 2007 e 2013, oito abordam o monitoramento desta droga e vinte e dois o desenvolvimento/teste de performance de novas técnicas. Estes resultados alertam para que maior número de estudos de monitoramento da tilosina no leite seja realizado a fim de se estabelecer políticas de segurança alimentar e controle dessa substância, além de investigar se há uso indiscriminado desta droga.Palavras-chave: leite, monitoramento, tilosina. Tylosin: an important antibiotic not monitored in milk in BrazilThe use of antibiotics in animal feed has brought changes to food production worldwide, but the indiscriminate use of these drugs can be harmful to human beings. In general, macrolides are the second class of antibiotics most used in animals, in which tylosin is found in a large number of formulations available for livestock. Contaminated milk by residues of tylosin above safe values can become a potential risk to the health of consumers. Due to the purpose of having an overview of the current food safety situation regarding this problem, this study was done based on a bibliographical survey at database SciFinder Scholar and periodicals from CAPES. It was found that in Brazil, there are two current programs

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Innovative mixture of salts in the quick, easy, cheap, effective, rugged, and safe method for the extraction of residual macrolides in milk followed by analysis with liquid chromatography and tandem mass spectrometry

Costa

Spisso

Pereira

et al. 2015

J of Separation Science

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A simple extraction technique has been developed for seven macrolide antibiotics in milk. The procedure involves a modified quick, easy, cheap, effective, rugged, and safe method based on acetonitrile extraction, followed by the addition of a mixture of salts (sodium sulfate, sodium chloride, and potassium carbonate) not yet reported in literature. The method was validated for tylosin and was selective, free of matrix effect, and linear in the range of 0.78-18.75 ng/mL in the final extract, corresponding to 0.125-3 times the maximum residue limit. The limit of detection, limit of quantification, decision limit, and detection capability were, respectively, 0.84, 2.79, 58.4, and 71.7 μg/kg. The overall average recovery at 25, 50, and 75 μg/kg ranged from 89-97%. Repeatability and intermediate precision expressed by relative standard deviations were below 10.5 and 12%, respectively. The extension of the validation for spiramycin, throleandomycin, oleandomycin, roxithromycin, erythromycin, and clarithromycin is under consideration since the procedure proved to be able to efficiently extract all studied macrolides, with recoveries from 74-104% at 50 μg/kg for tylosin, erythromycin, spiramycin, and oleandomycin and 20 μg/kg for throleandomycin, roxithromycin, and clarithromycin.

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Simultaneous separate and group determination of tylosin and tilmicosin in foodstuffs using single antibody-based immunoassay

Cited by 29 publications

References 19 publications

Group determination of 14-membered macrolide antibiotics and azithromycin using antibodies against common epitopes

Group determination of 14-membered macrolide antibiotics and azithromycin using antibodies against common epitopes

Tilosina: um importante antibiótico não monitorado em leite no Brasil

Innovative mixture of salts in the quick, easy, cheap, effective, rugged, and safe method for the extraction of residual macrolides in milk followed by analysis with liquid chromatography and tandem mass spectrometry

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