Simultaneous Spectrophotometric Determination of Diacerein and Celecoxib in Capsules by Chemometric Methods

Abstract

Simultaneous spectrophotometric determination of diacerein and celecoxib was performed by partial least-squares (PLS) and principal component regression (PCR) methods do not require any priori graphical treatment of the overlapping spectra of two drugs in the mixture. The absorbance values in the UV-Vis spectra were measured for the 64 wavelength points (from 225-288) in the spectral region 200–400 nm considering the intervals of 1 nm. The calibration range was found to be 2-10 μg/ml for diacerein, 4-20 μg/ml for celecoxib with a correlation coefficient of 0.9999(PLS), 0.9998(PCR) for diacerein and 0.9999(PLS) ,0.9999(PCR) for celecoxib. The validation of the multivariate methods was realized by analyzing the synthetic mixtures of diacerein and celecoxib. The numerical calculations were performed with the ‘Unscrambler 10.1 X’ software. The chemometrics analysis methods were satisfactorily applied to the simultaneous determination of diacerein and celecoxib in the pharmaceutical formulation

Chemometrics-assisted spectrophotometry for simultaneous determination of sodium benzoate and citric acid in beverage products

“…The determination of each compound in a mixture solutions that have overlapping spectra could only be solved by chromatographic separation methods or using a method of spectrophotometry combined with chemometrics approaches (Miller and Miller, 2010). This method could be used to determine the level of compound having spectrum overlap profile because it could separate the information with the noise part of the obtained data and could minimize the occurrence of error on the calibration model (Ragupathy and Arcot, 2013).…”