The stem bark of the plant Symplocos crataegoides Buch.-Ham. ex D. Don, Fam. Symplocaceae is extensively used in Indian medicine under the names of Lodhra/Patikalodhra. S. crataegoides is a deciduous shrub or a tree, distributed in the Himalayas from Punjab to Assam, Khasi hills and Burma. 1,2 As per Ayurvedic references the term Lodhra denotes the stem bark of Symplocos racemosa/Symplocos crataegoides and considered as most useful remedy for uterine complaints, vaginal and menstrual disorders. In Sanskrit the name Lodhra means that it stops ocular discharges. The drug is useful in digestive disorders, eye diseases and ulcers. Decoction of the bark is used as a gargle in bleeding gums. Its astringent property is utilized for curing loose motions. It is used as aphrodisiac, useful in dropsy, elephantiasis and in fat in urine (Lipiduria). The bark is used in the treatment of opthalmia, tonic and to prevent abortion. 1,3-7 The important Ayurvedic formulations in which Lodhra is used as one of the ingredients are Lodhrasavam, Gandhatailam, Dasamularistam, Draksadi kasayam, Pusyanugacurnam, etc. 8 The Nyagrodhadi Kvatha Curna contains both Symplocos racemosa and Symplocos GC-MS Analysis of n-hexane Extract of Stem Bark of Symplocos crataegoides Buch.-Ham. ex D. Don ABSTRACT Context: The stem barks of the plant Symplocos crataegoides Buch.-Ham. ex D. Don (syn. Symplocos paniculata (Thunb.) Miq.), Fam. Symplocaceae is extensively used in Indian medicine under the names of Lodhra/Patikalodhra. Mainly it is used to cure uterine complaints, vaginal and menstrual disorders. Aim: To investigate the phytochemicals from the n-hexane extract of the stem bark of Symplocos crataegoides Buch.-Ham. ex D. Don., using GC-MS analysis. Materials and Methods: Stem bark of S.crataegoides was extracted by Soxhlet extraction method using n-hexane. The extract was injected by splitless injection mode into the GC MS 5975 C Agilent equipped with a QP-5000 (quadrupole) Gas Chromatography-Mass Spectrometer. Results: Identification of 57 compounds from n-hexane extract. Those compounds were identified by close matches with standard MS spectra and compared with NIST-11 and WILEY library data. Undecane (7.51%) was found as major compound followed by Isopropyl myristate, Dodecane, 1,2,4-trimethyl-benzene, Octacosane, 2-methyl-decane, 2-ethyl-1,2-dimethyl-benzene, 1,2,3,5-tetramethyl-benzene etc., other constituents were found to be in traceable quantities. Conclusion: GC-MS analysis of S. crataegoides revealed certain interesting facts of presentation of various phytoconstituents in the stem bark. The presence of various phytoconstituents contributes to the medicinal activity of the plant.

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Simultaneous spectrophotometric determination of diacerein and aceclofenac was performed by partial least-squares (PLS) and principal component regression (PCR) methods do not require any priori graphical treatment of the overlapping spectra of two drugs in the mixture. The absorbance values in the UV-Vis spectra were measured for the 67 wavelength points (from 234-300) in the spectral region 200–400 nm considering the intervals of 1 nm. The calibration range was found to be 1-5 μg/ml for diacerein, 2-10 μg/ml for aceclofenac with a correlation coefficient of 0.9998(PLS), 0.9995(PCR) for diacerein and 0.9999 (PLS), 0.9997 (PCR) for aceclofenac. The validation of the multivariate methods was realized by analyzing the synthetic mixtures of diacerein and aceclofenac. The numerical calculations were performed with the ‘Unscrambler 10.1 X’ software. The chemometrics analysis methods were satisfactorily applied to the simultaneous determination of diacerein and aceclofenac in the pharmaceutical formulation

Simultaneous spectrophotometric determination of diacerein and celecoxib was performed by partial least-squares (PLS) and principal component regression (PCR) methods do not require any priori graphical treatment of the overlapping spectra of two drugs in the mixture. The absorbance values in the UV-Vis spectra were measured for the 64 wavelength points (from 225-288) in the spectral region 200–400 nm considering the intervals of 1 nm. The calibration range was found to be 2-10 μg/ml for diacerein, 4-20 μg/ml for celecoxib with a correlation coefficient of 0.9999(PLS), 0.9998(PCR) for diacerein and 0.9999(PLS) ,0.9999(PCR) for celecoxib. The validation of the multivariate methods was realized by analyzing the synthetic mixtures of diacerein and celecoxib. The numerical calculations were performed with the ‘Unscrambler 10.1 X’ software. The chemometrics analysis methods were satisfactorily applied to the simultaneous determination of diacerein and celecoxib in the pharmaceutical formulation

Simultaneous spectrophotometric determination of Rabeprazole sodium and Domperidone maleate was performed by partial least-squares (PLS) and principal component regression (PCR) methods do not require any priori graphical treatment of the overlapping spectra of two drugs in the mixture. The absorbance values in the UV-Vis spectra were measured for the 89 wavelength points (from 221-309) in the spectral region 200-400nm considering the intervals of 1nm. The calibration range was found to be 4-20μg/ml for Rabeprazole sodium, 6-30μg/ml for Domperidone maleate with a correlation coefficient of 0.9999 (PLS), 0.9999 (PCR) for Rabeprazole sodium and 0.9999 (PLS), 0.9999 (PCR) for Domperidone maleate. The validation of the multivariate methods was realized by analyzing the synthetic mixtures of Rabeprazole sodium and Domperidone maleate. The numerical calculations were performed with the 'Unscrambler 10.1X' software. The chemometrics analysis methods were satisfactorily applied to the simultaneous determination of Rabeprazole sodium and Domperidone maleate in the pharmaceutical formulation.