Simultaneous Spectrophotometric Estimation of Atenolol and Lercanidipine Hydrochloride in Combined Dosage Form

Abstract

Two simple, rapid, accurate and economic spectrophotometric methods have been developed for the determination of Atenolol (ATE) & Lercanidipine Hydrochloride (LER) in combined dosage form. The first method is Area under curve (AUC) and second is Absorption Corrected method. The wavelength ranges 260-264 & 280-285 were selected to determine Atenolol (ATE) & Lercanidipine (LER), respectively in combined formulation by AUC method. The second method was absorption corrected method in which both the drugs exhibited strong absorbance at 270.33 nm. However, LER exhibited strong absorbance at 353.93, at which ATE showed zero absorbance. Hence, 353.93 nm was selected as one wavelength for determination of LER without interference from ATE. Quantitative estimation of ATE was carried out by subtracting the absorption due to LER at 270.33 nm using experimentally calculated absorption factor. Beer's law was obeyed in the concentration range of 25-300 ì g/ml for ATE and 5-60 ì g/ml for LER for both the methods. The results of analysis have been validated statistically and recovery studies confirmed the accuracy and reproducibility of the proposed methods which were carried out by following ICH guidelines.

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“…Moreover, expensive instrumentation with high energy consumption and complicated set-up were used, which reduces the usability in quality control laboratories. On the other hand, there are several reported analytical methods for the assay of LER including: spectrophotometry [9][10][11][12][13][14][15], thin-layer chromatography (TLC) [16,17], HPLC [5,[18][19][20][21][22][23][24], high-performance thin-layer chromatography (HPTLC) [17], ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) [25,26], liquid chromatographytandem mass spectrometry (LC-MS/MS) [21,[27][28][29][30] and capillary electrophoresis [31].…”