Simultaneous Spectrophotometric Estimation of Bisoprolol Fumarate and Hydrochlorothiazide in Tablet Formulation using Partial Least-Squares, Principal Component Regression Multivariate Calibrations and RP-HPLC Methods

Abstract

The UV spectrophotometric analysis of a binary mixture containing bisoprolol fumarate (BS) and hydrochlorothiazide (HZ) by using multivariate calibration methods, such as principal component regression (PCR) and partial least-squares regression (PLS-1); and a graphical spectrophotometric method such as second derivative of the ratio spectra 2 DD was described. A calibration set of 22 reference samples was used to develop the models for PCR and PLS-1. The calibration models optimization was achieved through the proper wavelength ranges selection, and excluding any information about the interfering excipients. In addition, a High Performance Liquid Chromatography (HPLC) was developed to analyze the same mixture. The chromatographic separation was achieved on a reversed phase, RP 18 column with mobile phase consisting of acetonitrile-0.01 M KH2PO4 (40:60, v/v, and pH 3.5). Quantitation was achieved with UV detection at 232 nm based on peak area. The proposed methods were validated and successfully used for the analysis of laboratory-prepared mixtures and pharmaceutical formulations.

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“…The present study envisages developing and validating the Q-absorbance UV-spectrophotometric method for simultaneously estimating BF and CLD in their tablet formulation. Determination of BF alone and in combinations with other drugs is reported in the literature by various methods, that is, UV Spectrophotometry, [6][7][8][9] HPTLC, [10][11][12] HPLC, [13][14][15][16][17][18][19] and capillary electrophoresis. [20][21][22] Furthermore, determination of CLD alone or with other drugs by HPTLC, [23][24][25][26][27][28] HPLC, [29][30][31][32][33][34] and spectrophotometry [30,[35][36][37][38][39][40][41][42][43][44][45] is reported.…”

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“…It is frequently used alone or in combination with other medications for the treatment of hypertension, congestive heart failure, symptomatic edema, diabetes insipidus, renal tubular acidosis, hypoparathyroidism, edema, prevention of kidney stones and used in the treatment of osteoporosis 3 . There are many published methods for determination HCTZ in tablets and biological samples such as spectrophotometric 4 , TLC 5 , voltammetry 6 , GC 7 , flow injection 8 , polarography 9 , and HPLC 10-13 . LOP is an angiotensin II blocker and used mainly to treat high blood pressure (hypertension).…”

Development of thin layer chromatographic densitometric method for simultaneous quantification of Ivabradine HCl and Bisoprolol fumarate

“…The literature survey indicates that there was no analytical method published for simultaneous estimation of Ivabradine and BISO in combination. Several LC techniques for BISO or IVA alone and in combination with other drugs have been reported [9–40], therefore the thought of interest arose to develop and validate a precise, sensitive, and accurate method for simultaneous estimation of IVA and BISO which can be used for routine analysis in different pharmaceutical preparations.…”