Simultaneous spectrophotometric estimation of gatifloxacin and ornidazole in tablet dosage form

Abstract

Three simple, accurate and economic methods multicomponent, two wavelength and simultaneous equations using area under curve have been described for the simultaneous determination of gatifloxacin and ornidazole in tablet dosage form. Gatifloxacin shows absorption maximum at 287.5 nm and ornidazole shows absorption maximum at 319.5 nm in distilled water. Beer's law was obeyed in the concentration range of 2-20 µg/ml for gatifloxacin and 10-60 µg/ml for ornidazole. The methods allow rapid analysis of binary pharmaceutical formulation with accuracy. Results of analysis for three methods were validated statistically and by recovery studies and were found satisfactory.

Development and validation of RP-HPLC method for quantitative estimation of related substances in gatifloxacin drug substance

“…The objectives of the research work reported in this paper were to develop a suitable stabilityindicating LC method for analysis of Gatifloxacin and to validate the method for specificity, LOD,LOQ, linearity, precision, accuracy, and robustness to show the stability-indicating power of the method and to ensure compliance with ICH Guidelines [8][9][10][11][12].The molecule structure is shown in Figure : 1 Impurity profiling of active pharmaceutical ingredients (API) in both bulk material and formulations is one of the most challenging tasks. The presence of unwanted or in certain cases unknown chemicals, even in small amounts, may influence not only the therapeutic efficacy but also the safety of the pharmaceutical products.…”