Simultaneous stacking of cationic and anionic compounds in single run capillary zone electrophoresis by two-end field amplified sample injection

Hou, Xiaoling; Deng, Dayong; Wu, Xi; Lv, Yi; Zhang, Jiyou

doi:10.1016/j.chroma.2010.06.070

Cited by 21 publications

(18 citation statements)

References 43 publications

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“…The resultant frustrated flow profile is a superposition of a pluglike profile of EOF and a parabolic profile of pressure driven flow. 12,13 To reduce sample dispersion, EOF is typically suppressed or reduced with application of coating to the microchannel 14 or additive to the BGE. 15 In contrast, there are methodologies which exploit EOF to achieve the desired ion pre-concentration and transport effect [16][17][18] in a micro/nanofluidic channel.…”

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confidence: 99%

Electroosmotic flow hysteresis for dissimilar ionic solutions

Lim

Lam

2015

Biomicrofluidics

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Electroosmotic flow (EOF) with two or more fluids is commonly encountered in various microfluidics applications. However, no investigation has hitherto been conducted to investigate the hysteretic or flow direction-dependent behavior during the displacement flow of solutions with dissimilar ionic species. In this investigation, electroosmotic displacement flow involving dissimilar ionic solutions was studied experimentally through a current monitoring method and numerically through finite element simulations. The flow hysteresis can be characterized by the turning and displacement times; turning time refers to the abrupt gradient change of current-time curve while displacement time is the time for one solution to completely displace the other solution. Both experimental and simulation results illustrate that the turning and displacement times for a particular solution pair can be directional-dependent, indicating that the flow conditions in the microchannel are not the same in the two different flow directions. The mechanics of EOF hysteresis was elucidated through the theoretical model which includes the ionic mobility of each species, a major governing parameter. Two distinct mechanics have been identified as the causes for the EOF hysteresis involving dissimilar ionic solutions: the widening/sharpening effect of interfacial region between the two solutions and the difference in ion concentration distributions (and thus average zeta potentials) in different flow directions. The outcome of this investigation contributes to the fundamental understanding of flow behavior in microfluidic systems involving solution pair with dissimilar ionic species. V C 2015 AIP Publishing LLC. [http://dx

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confidence: 99%

Electroosmotic flow hysteresis for dissimilar ionic solutions

Lim

Lam

2015

Biomicrofluidics

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“…If the EOF is suppressed completely, the physical volume of sample that enters the capillary will be reduced and therefore longer injection times can be used that results in lower LODs. Hou et al recently described a suppressed EOF system to simultaneously stack cationic and anionic compounds in dual opposite end injection CE. Using model cationic (matrine and oxymatrine) and anionic (5‐sulfosalicylic acid) compounds, LODs of 20 and 60 ng/L were obtained for the cationic and anionic model compounds, respectively, an enhancement of 1003–1380.…”

Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

et al. 2013

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CE has been alive for over two decades now, yet its sensitivity is still regarded as being inferior to that of more traditional methods of separation such as HPLC. As such, it is unsurprising that overcoming this issue still generates much scientific interest. This review continues to update this series of reviews, first published in Electrophoresis in 2007, with updates published in 2009 and 2011 and covers material published through to June 2012. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction and sweeping. Attention is also given to online or inline extraction methods that have been used for electrophoresis.

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“…Ultrasonic extraction was the most frequently used method due to its efficiency and easy manipulation. Indeed, it was employed in about two‐thirds (180 out of 271 articles) of cited references (Supporting Information Tables 1–7) 4–271. Other conventional extraction methods included reflux 6, 14, 15, 21, 45, 74, 84, 87, 97, 117, 121, 123, 131, 134, 157, 162, 164, 169, 171, 172, 176, 179, 182–184, 205, 212, 227, 235, 236, 259, Soxhlet extraction 23, 89, 91, 128, 131, 244, heating extraction 22, 39, 51...…”

Section: Sample Preparationmentioning

confidence: 99%

CE and CEC analysis of phytochemicals in herbal medicines

et al. 2011

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CE and CEC, due to their versatility and high efficiency, have attracted great interest in the analysis of phytochemicals in herbs and their preparations. Previously, we reviewed the analysis of phytochemical bioactive compounds by CE in 2006 (Electrophoresis 2006, 27, 4808-4819) or CEC in 2010 (Electrophoresis 2010, 31, 260-277). This review followed the previous studies and covered the literature published since 2006 for CE and 2009 for CEC (excluding those mentioned in the two previous reviews), which emphasized the development of CE and CEC techniques in phytochemical analysis. In addition, sample preparation and detection were also discussed.

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Simultaneous stacking of cationic and anionic compounds in single run capillary zone electrophoresis by two-end field amplified sample injection

Cited by 21 publications

References 43 publications

Electroosmotic flow hysteresis for dissimilar ionic solutions

Electroosmotic flow hysteresis for dissimilar ionic solutions

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

CE and CEC analysis of phytochemicals in herbal medicines

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