Simultaneous TLC–densitometric determination of tamoxifen citrate and medroxyprogesterone acetate and UV‐degradation kinetic study of medroxyprogesterone acetate

Abstract

Coadministration of tamoxifen citrate (TMC) and medroxyprogesterone acetate (MPA) is preferred to increase the response rate and the percentage recovery in patients with endometrial carcinoma. Administration of TMC and MPA and their combination affects estrogen and progestin receptor concentrations in advanced endometrium carcinoma by affecting 17β-hydroxyl steroid dehydrogenase activity and serum hormone concentrations. A sensitive, accurate and robust thin-layer chromatography method has been established for simultaneous analysis of TMC and MPA. Method development was carried out on silica gel F using butanol-acetic acid-water (6:0.5:0.5, v/v/v) as mobile phase. Densitometric scanning was carried out at 241 nm for simultaneous detection of TMC and MPA. Retardation factor (R ) values for TMC and MPA were 0.21 and 0.85, respectively. The method was validated according to ICH guidelines. Regression plots revealed linear relationships in the concentration range of 50-500 and 25-250 ng/band for TMC and MPA, successively. Accuracy was ≥99.60 and 98.72% for TMC and MPA, respectively. Forced degradation studies using UV photodegradation was applied on MPA after exposure to UV light for different times and applying a kinetic study for calculating the degradation rate constant (k) and half-life time (t ).

Cyclic and Linear Sweep Voltammetric Studies of a Modified Carbon Paste Electrode with Nickel Oxide Nanoparticles toward Tamoxifen: Effects of Surface Modification on Electrode Response Kinetics

“… 3 Unfortunately, some major side effects, including thromboembolic diseases and endometrial cancer, have limited the use of TAM in healthy women. 6 Severe side effects of TAM are known as its proliferative effect on the endometrium, which is dose-dependent. This reveals that TAM formulation and lower TAM dose with colloidal delivery systems are influential factors for long-term breast cancer chemoprevention.…”

“…In addition, these complex compounds cannot bind to the receptor sites in chromatin and can block estrogen action in the uterus and the breast . Unfortunately, some major side effects, including thromboembolic diseases and endometrial cancer, have limited the use of TAM in healthy women . Severe side effects of TAM are known as its proliferative effect on the endometrium, which is dose-dependent.…”

Analysis of tamoxifen and its main metabolites in plasma samples of breast cancer survivor female athletes: Multivariate and chemometric optimization

“…Regarding chromatography methods, such as liquid, gas, thin layer, and micellar chromatography combined with different detection systems, a number of analytical techniques have been implemented for separating and quantifying TAM and its metabolites [9][10][11][12][13]. Since these compounds are nonvolatile, the use of GC to analyze them requires a derivation step that is extremely time consuming, does not meet the principles of green chemistry, and has not been well received by researchers [10,11].…”

The Experimental Design Approach in Square‐Wave Voltammetric Determination of Tamoxifen by NiO‐CPE**

“…Thus, they have a high capability for blocking the action of estrogen in the uterus and the breast [1] . The use of this drug in healthy women has been limited by side effects, such as thromboembolic diseases [9] . One of the dose‐dependent side effects of TAM is its proliferative endometrium effect.…”

“…[1] The use of this drug in healthy women has been limited by side effects, such as thromboembolic diseases. [9] One of the dose-dependent side effects of TAM is its proliferative endometrium effect. TAM formulation has confirmed that its lower dose with colloidal delivery systems is an influencing factor for long-term chemoprevention of breast cancer.…”