Simultaneous ultrasound-assisted water extraction and β-cyclodextrin encapsulation of polyphenols fromMangifera indicastem bark in counteracting TNFα-induced endothelial dysfunction

Abstract

This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of β-cyclodextrin (β-CD) at room temperature and testing their biological activity on TNFα-induced endothelial dysfunction. Both extracts counteracted TNFα effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels. β-CD extract showed higher efficacy in improving endothelial function. These effects were abolished after pre-treatment with the oestrogen receptor inhibitor ICI1182,780. Moreover, the β-CD extract induced Akt activation and completely abolished the TNFα-induced p38MAPK phosphorylation. UAE and β-CD encapsulation provide an efficient extraction protocol that increases polyphenol bioavailability. Polyphenols from MI play a protective role on endothelial cells and may be further considered as oestrogen-like molecules with vascular protective properties.

Consumption of a Mango Fruit Powder Protects Mice from High-Fat Induced Insulin Resistance and Hepatic Fat Accumulation

“…Moreover, a recent intervention study in humans reported moderate beneficial effects of the same mango fruit powder on microcirculation and endothelial function [18], mediated, at least partly, by the activation of endothelial nitric oxide synthase [18]. Similar results from studies with rats [15] and human umbilical vein cells [20] also suggest that polyphenols of aqueous extracts of Mango may protect endothelial cells. These results are of interest considering that impaired microvascular function is observed early in the development of type 2 diabetes mellitus and cardiovascular disease [21].…”

Analysis of the intricate effects of polyunsaturated fatty acids and polyphenols on inflammatory pathways in health and disease

“…Exposure to the extracts from Brassica oleracea significantly reduced the TNF-α induced expression of E-selectin, ICAM-1 and VCAM-1 (Kuntz and Kunz, 2014). Another study demonstrated the capacity of extracts from Mango (Mangifera indica L.) to counteract TNF-α effects on HUVECs, through the inhibition of IL-6, IL-8, COX-2 and ICAM-1, while restoring the expression of eNOS usually down-regulated by TNF-α (Mura et al, 2015). Interestingly, in an animal model, for example, supplementation of standard chow with cacao phenolic compounds in apolipoprotein E-deficient mice, lead to an anti-inflammatory effect justified by a reduction of VCAM-1 and ICAM-1 expressions, a decrease of oxidative stress markers and a reduction of cholesterol accumulation in plaque compared to control (Natsume and Baba, 2014).…”

Innovative methods for extraction of essential oils from medicinal plants

“…The efficiency of extraction can be enhanced using this technique which may cause disruption of plant cell walls and improved mass transfer through the formation of cavitation babble effects (Entezari, 2004). The disadvantages of this method include poor purity, low efficiency, and lengthy process (Lu et al, 2012;Mura et al, 2015). Antimicrobial, antioxidant, antiinflammatory and anxiolytic activity (Arafat et al, 2020;Mahmud et al, 2009)…”