Simvastatin Induces In Vitro Mineralization Effects of Primary Human Odontoblast-Like Cells

Sabandal, Martin M. I.; Schäfer, Edgar; Imper, Jessica; Jung, Susanne; Kleinheinz, Johannes; Sielker, Sonja

doi:10.3390/ma13204679

Cited by 3 publications

(18 citation statements)

References 51 publications

(174 reference statements)

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“…Sabandal et al . found similar results in their study for a concentration of 1.0 µM SV when using odontoblast-like cells, so this could confirm a dose-dependent reduction in cell viability as well [11].…”

Section: Discussionsupporting

confidence: 70%

“…The current study is based on previous published studies investigating pleiotropic effects on primary human odontoblast-like cells and primary human osteoblasts under influence of SV [8,11]. These previous studies showed the necessary time of 9 [8] to 14 [11] days to induce mineralization. Additionally performed preliminary studies regarding die induction of osteoblastic differentiation of hADSC (data not shown) showed additionally needed time for osteoblastic differentiation up to 4 to 9 days.…”

Section: Discussionmentioning

confidence: 99%

“…According to the methodology of other studies the cell viability after exposition to SV was performed within the present investigation [8,11]. The investigation period ranged up to 28 days, whereas similar studies investigated the cell viability from day 1 [7] up to 9 days [5].…”

Section: Discussionmentioning

confidence: 99%

“…At the same point of time, there was also a significant ( p < 0.01) increase of the mineralization of the group with 2.0 µM SV compared to all other groups (figure 3). In comparison, mineralization under the influence of SV in human osteoblasts and human odontoblast-like cells already occurred on day 16 and day 21, respectively [8,11].…”

Section: Discussionmentioning

confidence: 99%

“…Osteogenic activity was analysed using an alkaline phosphatase (ALP) assay and total protein was analysed with a Pierce BCA Protein Assay (Thermofisher Scientific), in which the protein content is determined by calorimetric measurement of Cu 1+ ions by bicinchoninic acid (BCA). All assays have been described previously [8,11]. SPSS v. 27 (IBM, Ehningen, Germany) was used for statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

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Pleiotropic effects on proliferation and mineralization of primary human adipose tissue-derived stromal cells induced by simvastatin

et al. 2022

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The circulating low-density lipoprotein concentration in blood can be reduced by the administration of statins. Frequently simvastatin (SV) is prescribed. Due to the reported pleiotropic effects of SV the aim of this study was to evaluate mineralization effects on human adipose tissue-derived stromal cells upon administration of SV. After informed consent human adipose tissue-derived stromal cells were obtained from tissue surplus of regular treatments of 14 individuals. According to established protocols after adding various SV concentrations (0.01 µM, 0.1 µM, 1.0 µM, 2.0 µM), alkaline phosphate (osteoblastic marker), mineralization capability and viability were determined at day 18, 21 and 28. The Kruskal–Wallis test was performed for statistical analysis. After adding SV a dose-dependent significant decreased viability and levels of alkaline phosphatase ( p < 0.01) and a significantly increased mineralization ( p < 0.01) of the primary cultures was recognized during the late mineralization stage. Mineralization of the human adipose tissue-derived stromal cells was induced by SV, possibly originated from alternative pathways than the alkaline phosphatase pathway. Further investigations should be performed regarding switching into the osteoblastic differentiation and as a possible source of cells that can be used as the basis for a potential bone graft substitute, which may allow an extension of the field of application.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Pleiotropic effects on proliferation and mineralization of primary human adipose tissue-derived stromal cells induced by simvastatin

et al. 2022

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Statins—Their Role in Bone Tissue Metabolism and Local Applications with Different Carriers

Granat,

Eifler-Zydel,

Kolmas

2024

IJMS

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Statins, widely prescribed for lipid disorders, primarily target 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase competitively and reversibly, resulting in reduced low-density lipoprotein cholesterol (LDL-C). This mechanism proves effective in lowering the risk of lipid-related diseases such as ischemic cerebrovascular and coronary artery diseases. Beyond their established use, statins are under scrutiny for potential applications in treating bone diseases. The focus of research centers mainly on simvastatin, a lipophilic statin demonstrating efficacy in preventing osteoporosis and aiding in fracture and bone defect healing. Notably, these effects manifest at elevated doses (20 mg/kg/day) of statins, posing challenges for systematic administration due to their limited bone affinity. Current investigations explore intraosseous statin delivery facilitated by specialized carriers. This paper outlines various carrier types, characterizing their structures and underscoring various statins’ potential as local treatments for bone diseases.

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Human Primary Odontoblast-like Cell Cultures—A Focused Review Regarding Cell Characterization

Klein¹,

Meller²,

Schäfer

2022

JCM

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Cell cultures can provide useful in vitro models. Since odontoblasts are postmitotic cells, they cannot be expanded in cell cultures. Due to their extension into the dentin, injuries are inevitable during isolation. Therefore, “odontoblast-like” cell culture models have been established. Nowadays, there is no accepted definition of odontoblast-like cell cultures, i.e., isolation, induction, and characterization of cells are not standardized. Furthermore, no quality-control procedures are defined yet. Thus, the aim of this review was to evaluate both the methods used for establishment of cell cultures and the validity of molecular methods used for their characterization. An electronic search was performed in February 2022 using the Medline, Scopus, and Web of Science database identifying publications that used human primary odontoblast-like cell cultures as models and were published between 2016 and 2022. Data related to (I) cell culture conditions, (II) stem cell screening, (III) induction media, (IV) mineralization, and (V) cell characterization were analyzed. The included publications were not able to confirm an odontoblast-like nature of their cell cultures. For their characterization, not only a similarity to dentin but also a distinction from bone must be demonstrated. This is challenging, due to the developmental and evolutionary proximity of these two tissue types.

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Simvastatin Induces In Vitro Mineralization Effects of Primary Human Odontoblast-Like Cells

Cited by 3 publications

References 51 publications

Pleiotropic effects on proliferation and mineralization of primary human adipose tissue-derived stromal cells induced by simvastatin

Pleiotropic effects on proliferation and mineralization of primary human adipose tissue-derived stromal cells induced by simvastatin

Statins—Their Role in Bone Tissue Metabolism and Local Applications with Different Carriers

Human Primary Odontoblast-like Cell Cultures—A Focused Review Regarding Cell Characterization

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