Single analysis of human hair for determining halogens and sulfur after sample preparation based on combustion reaction

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Rationale To increase the consumption of egg powder and its fractions a suitable quality control method is required to obtain more information on its nutritional composition. The proposed method enables the quantification of important elements for the functioning of the human organism, such as halogens and sulfur, in egg powder and its fractions. Methods Up to 350 mg of egg powder or its fractions (egg white powder and egg yolk powder) were digested by microwave‐induced combustion using 20 bar pressure of oxygen. The analytes were absorbed in 100 mmol L−1 of NH4OH solution. The determination of halogens (chlorine, bromine, fluorine, and iodine) and sulfur was performed in a single analysis using ion chromatography with conductivity detection coupled to mass spectrometry. Results Using the proposed method, spike recoveries between 99% and 104% for all analytes were obtained, and results agreed with certified values of reference materials (agreements were between 100% and 109%). The relative standard deviations were below 8%. The variation in elemental concentration over a wide range in different fractions (whole egg powder, egg white powder, and egg yolk powder) and different brands was also observed. Conclusions The proposed method provides reliable information about minerals in whole egg powder and its fractions, contributing to better quality control of these products. Because these food products are widely consumed, these results suggest the safe ingestion levels of these elements.

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ion chromatography coupled to mass spectrometry as a powerful technique for halogens and sulfur determination in egg powder and its fractions

Mesko

Toralles

Coelho

et al. 2020

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“…1,2 Moreover, bromine and iodine determination by ICP-MS is also impaired by residual acidity and carbon content in the final solution. 3,4,16 To meet the current demand, some analytical methods for determining halogens in biological samples at very low concentrations, with suitable accuracy, were recently proposed by our research group, 3,4,17 and they were successfully accepted by the scientific community. However, there is a lack of suitable methods for bromine and iodine determination in human nail samples.…”

Section: Introductionmentioning

confidence: 99%

“…16,[19][20][21] However, the use of concentrated acids in conventional wet digestion methods results in digests with high residual acidity, causing the formation of volatile bromine and iodine species. [1][2][3][4]17,22 Extraction methods using alkaline solutions can avoid the formation of the volatile species, but the high carbon content in the final solution can impair the determination step, including ICP-MS analysis. [1][2][3][4]17 Microwave-induced combustion (MIC) is an excellent alternative as a sample preparation method for further determination of bromine and iodine.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of microwave‐induced combustion combined with inductively coupled plasma mass spectrometry for bromine and iodine determination in human nail

Novo

Henn

Flores

et al. 2020

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Rationale Bromine and iodine have important physiological functions; however, in inadequate concentration, they can also cause several physiological problems. Their mobility assessment in human organisms through biological sampling may help clarify some doubts related to metabolic routes, which are still not well elucidated. In this context, a suitable analytical method for this purpose should be developed. Methods An analytical method for determining ultratrace levels of bromine and iodine in human nail samples was developed. Inductively coupled plasma mass spectrometry (ICP‐MS) using a conventional nebulization system was immediately chosen as the determination tool because of its powerful sensitivity and selectivity. Sample preparation methods including microwave‐induced combustion (MIC), microwave‐assisted extraction, and microwave‐assisted digestion were evaluated. The compatibility of the final solutions with ICP‐MS analysis was considered while the method was developed. Results MIC was chosen as the most suitable method for the sample preparation for determining the levels of bromine and iodine in human nail samples using ICP‐MS. Unlike other sample preparation methods, this one fully eliminated interferences related to the carbon content and memory effects. Sample masses up to 100 mg were efficiently digested, and the analytes were quantitatively absorbed using only 50 mmol L−1 NH4OH solution. Recoveries ranged from 93% to 102%, and the relative standard deviation was < 8%. Conclusions The proposed analytical method presents important characteristics for routine analysis. It allows ultratrace determination even when low sample masses are used because of the low blank values, reduced volume of reagents, and powerful detectability using ICP‐MS.

Challenges and trends for halogen determination by inductively coupled plasma mass spectrometry: A review

Flores

Mello

Krzyzaniak

et al. 2020

Rationale In this review, works published in the past 25 years for fluorine, chlorine, bromine, and iodine determination in several matrices by inductively coupled plasma mass spectrometry (ICP‐MS) were covered. Usually, the determination of halogens has been performed by ICP‐MS using a previous sample preparation step or, more recently, by direct analysis of solid or liquid samples. Methods Methods based on combustion, extraction, pyrohydrolysis, sample dilution in organic or aqueous medium, and wet digestion, among others, are discussed. Moreover, the recent applications of methods based on laser ablation (LA) and electrothermal vaporization (ETV) coupled to ICP‐MS are discussed. Results The main challenge for methods using sample preparation has been to obtain a final solution compatible with ICP‐MS, as well as to overcome problems related to analyte losses and contamination. Interferences due to the presence of dissolved organic compounds in solution, enhancement or suppression of ionization of analytes, and related matrix effects have been of concern when using ICP‐MS. For the determination of halogens by ICP‐MS using LA and ETV systems, some limitations related to the difficulty of calibration are pointed out, impairing the widespread use of this approach. Conclusions A critical view is presented for further halogen determination by ICP‐MS, mainly for matrices considered difficult to digest using conventional protocols.