Single and 14-Day Repeated Oral Toxicity Studies of 70% Ethanol Extract of Lindera Obtusiloba Blume Leaves

홍충의,; 서문영,; 구윤창,; 남미현,; 이현아,; 김지훈,; 왕증,; 양성용,; 이성희,; 노수환,; 이광원,

doi:10.3746/jkfn.2009.38.10.1324

Cited by 7 publications

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A safety assessment showed that LOLE had no significant cytotoxic effects up to 0.25 mg/mL, suggesting that it could be used to assess anti-noroviral activity without significant cytotoxicity in RAW 264.7 cells. Hong et al (2009) reported no adverse effects of LOLE consumption in Sprague-Dawley rats fed with the extract (2000 mg/kg daily) for 14 days [ 26 ]. Other studies focusing on the therapeutic properties of LOLE using in vivo models did not report any negative effects of the extract on the health of the animals [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Antiviral Effects of Lindera obtusiloba Leaf Extract on Murine Norovirus-1 (MNV-1), a Human Norovirus Surrogate, and Potential Application to Model Foods

et al. 2020

View full text Add to dashboard Cite

Noroviruses are the leading cause of acute gastroenteritis and food poisoning worldwide. In this study, we investigated the anti-noroviral activity of Lindera obtusiloba leaf extract (LOLE) using murine norovirus (MNV-1), a surrogate of human norovirus. Preincubation of MNV-1 with LOLE at 4, 8, or 12 mg/mL for 1 h at 25 °C significantly reduced viral infectivity, by 51.8%, 64.1%, and 71.2%, respectively. Among LOLE single compounds, β-pinene (49.7%), α-phellandrene (26.2%), and (+)-limonene (17.0%) demonstrated significant inhibitory effects on viral infectivity after pretreatment with MNV-1, suggesting that the anti-noroviral effects of LOLE may be due to the synergetic activity of several compounds, with β-pinene as a key molecule. The inhibitory effect of LOLE was tested on the edible surfaces of lettuce, cabbage, and oysters, as well as on stainless steel. After one hour of incubation at 25°C, LOLE (12 mg/mL) pretreatment significantly reduced MNV-1 plaque formation on lettuce (76.4%), cabbage (60.0%), oyster (38.2%), and stainless-steel (62.8%). These results suggest that LOLE effectively inhibits norovirus on food and metal surfaces. In summary, LOLE, including β-pinene, may inactivate norovirus and could be used as a natural agent promoting food safety and hygiene.

show abstract

Section: Discussionmentioning

confidence: 99%

Antiviral Effects of Lindera obtusiloba Leaf Extract on Murine Norovirus-1 (MNV-1), a Human Norovirus Surrogate, and Potential Application to Model Foods

et al. 2020

View full text Add to dashboard Cite

show abstract

Repeated-dose oral toxicity study of crude antifungal compounds produced by Lactobacillus plantarum AF1 in rats

Lee¹,

Lee²,

Chang³

et al. 2013

Korean Journal of Food Preservation

View full text Add to dashboard Cite

Repeated-Dose Oral Toxicity Study of Lactobacillus Plantarum AF1 Isolated from Kimchi in Rats

Lee¹,

Lee²,

Son³

et al. 2012

Journal of the Korean Society of Food Science and Nutrition

View full text Add to dashboard Cite

This study was performed to investigate the 4-week repeated-dose toxicity of Lactobacillus plantarum AF1 (Lb. plantarum AF1), a lactic acid bacteria isolated from kimchi, in male and female rats. Sprague-Dawley male and female rats were divided into four groups, with 10 animals in each group. The test article was administered once daily by gavage to rats at dosage levels of 0, 0.5, 1.0, and 2.0 g/kg/day for 4-weeks. There were no test articlerelated deaths or abnormal clinical signs in either gender of rat during the observation period. Furthermore, no differences were found between the control and treatment groups in terms of body weight changes, food intake, and water consumptions. Hematological parameters, serum biochemical analysis, and any other findings also showed no significant or dose-dependent alterations. There were no alterations in organ weights upon administration of Lb. plantarum AF1. These results suggest that there were no adverse effects of oral application of Lb. plantarum AF1 up to a dosage level of 2.0 g/kg in both male and female rats.

show abstract

Single and 14-Day Repeated Oral Toxicity Studies of 70% Ethanol Extract of Lindera Obtusiloba Blume Leaves

Cited by 7 publications

References 6 publications

Antiviral Effects of Lindera obtusiloba Leaf Extract on Murine Norovirus-1 (MNV-1), a Human Norovirus Surrogate, and Potential Application to Model Foods

Antiviral Effects of Lindera obtusiloba Leaf Extract on Murine Norovirus-1 (MNV-1), a Human Norovirus Surrogate, and Potential Application to Model Foods

Repeated-dose oral toxicity study of crude antifungal compounds produced by Lactobacillus plantarum AF1 in rats

Repeated-Dose Oral Toxicity Study of Lactobacillus Plantarum AF1 Isolated from Kimchi in Rats

Contact Info

Product

Resources

About