Single and co-inoculation of Bacillus subtilis and Azospirillum brasilense on Lycopersicon esculentum: Effects on plant growth and rhizosphere microbial community

Felici, Cristiana; Vettori, L. Pecori; Giraldi, Enrico; Forino, Laura Maria Costantina; Toffanin, Annita; Tagliasacchi, A. M.; Nuti, Marco

doi:10.1016/j.apsoil.2008.05.002

Cited by 117 publications

(48 citation statements)

References 31 publications

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“…In some cases, a strong and persistent effect was observed (12,39,45,46). However, in the majority of cases inoculation with plant growth-promoting bacterial strains resulted in undetectable or only minor and transient shifts in root and rhizosphere bacterial communities (4).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Effects of Compost Amendment and of Single-Strain Inoculation on Root Bacterial Communities of Young Cucumber Seedlings

Ofek

Hadar

Minz

2009

Appl Environ Microbiol

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Compost amendment and inoculations with specific microorganisms are fundamentally different soil treatment methods, commonly used in agriculture for the improvement of plant growth and health. Although distinct, both methods affect the rhizosphere and the plant roots. In the present study we used a 16S rRNA gene approach to achieve an overview of early consequences of these treatments on the assemblage of plant root bacterial communities. For this purpose, cucumber seedlings were grown, under controlled conditions, in perlite potting mix amended with biosolid compost or straw compost, or inoculated with Streptomyces spp. A uniform trend of response of root bacterial communities for all treatments was observed. Root bacterial density, measured as bacterial targets per plant tef gene by real-time PCR, was reduced in 31 to 67%. In addition, increased taxonomic diversity accompanied shifts in composition (␣-diversity). The magnitude of change in these parameters relative to the perlite control varied between the different treatments but not in relation to the treatment method (compost amendments versus inoculations). Similarity between the compositions of root and of potting mix bacterial communities (␤-diversity) was relatively unchanged. The abundance of Oxalobacteraceae was >50% of the total root bacterial community in the untreated perlite. Root domination by this group subsided >10-fold (straw compost) to >600-fold (Streptomyces sp. strain S1) after treatment. Thus, loss of dominance appears to be the major phenomenon underlining the response trend of the root bacterial communities.Environmental concern over conventional agricultural fertilization and disease control measures has led to increased interest in finding environmentally friendly alternatives. The most explored ones include compost amendments (18, 36) and the application of different microbial preparations (11,19,37). These are widely distinct applications. The first approach adds to the amended medium not only a rich and diverse consortium of biological agents but also organic matter and nutrients. It was confirmed that the efficacy of such treatments involves the response of the soil, the plant, and the rhizosphere microbial communities (19,56). The activities of rhizosphere microorganisms alter the rhizosphere and thus affect plant health and root growth and development (24). Therefore, one of the main objectives of compost amendment or of inoculation with specific microbial strains is manipulation of the plant rhizosphere conditions, particularly via manipulation of the microbial community composition (32).The response of rhizosphere bacterial communities to different anthropogenic and other disturbances has been discussed in terms of resilience (3,32). Generally, the introduction of new microorganisms produces only restricted spatial and temporal effects on the soil, rhizosphere, and root microbial communities (4,29,35). Thus, the plant growth-promoting effect of such treatments may be related to microbial events occurring during the early stage...

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Section: Discussionmentioning

confidence: 99%

Comparison of Effects of Compost Amendment and of Single-Strain Inoculation on Root Bacterial Communities of Young Cucumber Seedlings

Ofek

Hadar

Minz

2009

Appl Environ Microbiol

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“…However, most approaches for using PGPB have relied on application of single bacterial strains that may partially account for the reported inconsistencies in field, since a single biological agent is not likely to be active in all soil environments. Logically, potentials for improving plant yields by combining these PGPB by co-inoculation have also been a subject of several investigations for more than a decade (Felici et al 2008;Cong et al 2009). Inoculation of Azospirillum the well-known PGPB is more successful and more profitable when it is applied along with other groups of microorganisms (see Bashan et al 2004).…”

Section: Introductionmentioning

confidence: 98%

Effect of co-inoculation of methylotrophic Methylobacterium oryzae with Azospirillum brasilense and Burkholderia pyrrocinia on the growth and nutrient uptake of tomato, red pepper and rice

Madhaiyan

Prabakaran

Kang³

et al. 2009

Plant Soil

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The present greenhouse study was undertaken to evaluate the effects of co-inoculating methylotrophic Methylobacterium oryzae CBMB20 along with nitrogen-fixing Azospirillum brasilense CW903 or a phosphate solubilizing bacterium Burkholderia pyrrocinia CBPB-HOD on the growth and nutrient uptake of tomato, red pepper and rice. Seed inoculation and soil/foliar application of the bacterial strains alone or under dual inoculation increased the plant growth in terms of shoot or root length and increased the nutrient uptake in the plants studied compared to uninoculated control plants. Co-inoculation of M. oryzae CBMB20 with A. brasilense CW903 or B. pyrrocinia CBPB-HOD improved the N and P concentration of plants, while the results varied among the plant species tested. Also, co-inoculation of the bacterial strains increased the activity of nitrogenase, urease and phosphatase enzymes in soil when compared to uninoculated control or individual inoculations. Though the inoculation effects were analyzed at an early stage of plant growth, the results conclusively suggest that M. oryzae being compatible with other microorganisms in the rhizosphere can potentially be used as individual inoculant or co-inoculated with other plant growth promoting bacteria to increase the production in sustainable agricultural systems.

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“…Similarly, increases in the other variables studied were recorded, although the only statistically significant differences (p<0.05) are the length and stem wet weight (Table 3) DISCUSSION Bacillus is one of the most studied genus due to the plant growth promoting capacity in crops of economic importance (Rojas et al, 2011;Hernández-Rodríguez et al, 2014;Myresiotis et al, 2014;Widnyana Ketut and Cokorda Javandira, 2016). These bacteria are not usually important producers of auxin, however, some strains with the capacity to produce between 16 and 55 µg/ml of IAA have been reported (Tsavkelova, 2006;Felici et al, 2008;Rojas et al, 2011). The genus Bacillus includes B. megaterium species and B. subtilis that can produce organic acids as primary mechanism of solubilization of phosphate, but may also act as phytases enzymes excreted into the culture medium (Fernandez et al, 2005).…”

Section: Effect Of Bacillus Subtilis Strain Gibi 200 On Tomato Cropmentioning

confidence: 99%

Bacillus effect on the germination and growth of tomato seedlings (Solanum lycopersicum L).

et al. 2017

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The capacity to solubilize phosphate and to produce indole compounds Indole Acetic Acid type, was evaluated in 15 strains isolated from castor bean lignocellulosic residues (Ricinus communis). To determine the solubilizing activity of phosphates a qualitative test by using Pikovskaya culture medium was employed and for the evaluation of the production of indole compounds (IAA) a Salkowsky colorimetric analysis technique was applied. Among the microorganisms tested, the Bacillus pumilus GIBI 206 demonstrated capacity to solubilize phosphates and Bacillus subtilis GIBI 200 showed of capacity to solubilize phosphates and to produce Indoleacetic Acid (IAA). To determine the effect of the Bacillus subtilis strain on germination and growth promotion, tomato seeds (Solanum lycopersicum 'Santa Clara') were inoculated; the inoculation of the seeds along with the microorganism revealed statistically significant differences, during the germination stage compared to the control treatment. Nevertheless, it revealed a positive influence on the development of tomato plants, originating a significant increase on the mass and length of its stem and root. The results of this research offer the possibility of using the Bacillus subtilis as a growth promoter in tomato seedlings and in the formulation of bio-products.

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Single and co-inoculation of Bacillus subtilis and Azospirillum brasilense on Lycopersicon esculentum: Effects on plant growth and rhizosphere microbial community

Cited by 117 publications

References 31 publications

Comparison of Effects of Compost Amendment and of Single-Strain Inoculation on Root Bacterial Communities of Young Cucumber Seedlings

Comparison of Effects of Compost Amendment and of Single-Strain Inoculation on Root Bacterial Communities of Young Cucumber Seedlings

Effect of co-inoculation of methylotrophic Methylobacterium oryzae with Azospirillum brasilense and Burkholderia pyrrocinia on the growth and nutrient uptake of tomato, red pepper and rice

Bacillus effect on the germination and growth of tomato seedlings (Solanum lycopersicum L).

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