Single and combined exposures of waterborne Cu and Cd induced oxidative stress responses and tissue injury in female rare minnow (Gobiocypris rarus)

Wu, Lian; Yu, Qiuguo; Guo, Zhiyong; Wu, Feili; Zhang, Yingying; Yuan, Cong; Zhang, Ting; Wang, Zaizhao

doi:10.1016/j.cbpc.2019.04.013

Cited by 21 publications

(8 citation statements)

References 58 publications

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“…During the 96-h exposure to Cu 2+ , the MDA content in the liver and gill tissues of the wild-type group tended to increase, indicating that Cu 2+ induced lipid oxidation in both hepatocytes and gill cells. Similar results have reported in studies on other fish such as Channa punctata , Paralichthys olivaceus , and Gobiocypris rarus (Pandey et al, 2008; Cao et al, 2010; Wu et al, 2019). The enhanced antioxidant enzyme activities in the liver and gill tissues of the wild-type group at 48 h of exposure to Cu 2+ alleviated oxidative damage; however, damage to the liver and gill tissues had increased by 96 h of Cu 2+ exposure.…”

Section: Discussionsupporting

confidence: 90%

miR-489-3p Regulates the Oxidative Stress Response in the Liver and Gill Tissues of Hybrid Yellow Catfish (Pelteobagrus fulvidraco♀ × P. vachelli♂) Under Cu2+ Exposure by Targeting Cu/Zn-SOD

et al. 2019

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Copper/zinc superoxide dismutase (Cu/Zn-SOD) plays critical roles in protecting cells and tissues against oxidative damage. Excessive copper ions (Cu 2+ ) in water can damage the cells of aquatic organisms, leading to impaired growth and development and reduced antioxidant defenses. Many regulatory factors control the response to excess Cu 2+ . Among them, microRNAs (miRNAs) are important small RNAs that regulate the expression of their target genes and participate in the oxidative stress response. In the present study, we used bioinformatics and dual luciferase reporter gene analyses to demonstrate that the miR-489-3p of hybrid yellow catfish ( Pelteobagrus fulvidraco ♀ × P. vachelli ♂) binds to the 3′-untranslated region (UTR) of its target gene, which encodes a Cu/Zn-SOD. The regulatory relationship between this miRNA and its target gene Cu/Zn-SOD was analyzed using qRT-PCR and luciferase activity assays. We also investigated the effect of the loss of miR-489-3p expression on the oxidative stress response of hybrid yellow catfish exposed to Cu 2+ . The Cu/Zn-SOD 3′UTR region was found to be fully complementary to positions 2–9 of the 5′-end seed region of miR-489-3p. The miR-489-3p expression levels were negatively related to Cu/Zn-SOD expression. Silencing of miR-489-3p up-regulated Cu/Zn-SOD expression in the liver and gill tissues, increased activities of SOD and catalase, and reduced the malondialdehyde content. This study is the first to demonstrate that miR-489-3p targets Cu/Zn-SOD to mediate the oxidative response to metal stress. These findings provide a theoretical basis for further studies on the response to oxidative stress caused by metals in cultured fish, and provide an experimental basis for the management of the culture environment.

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Section: Discussionsupporting

confidence: 90%

miR-489-3p Regulates the Oxidative Stress Response in the Liver and Gill Tissues of Hybrid Yellow Catfish (Pelteobagrus fulvidraco♀ × P. vachelli♂) Under Cu2+ Exposure by Targeting Cu/Zn-SOD

et al. 2019

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“…In our study, excessive V elevated the levels of H 2 O 2 , MDA, and CAT, but increased T-SOD activity at 22 days and decreased at 44 days. According to the previous study, the antioxidant enzyme activities were enhanced but decreased after a while when the body suffered different degrees of oxidative damage (42,43). Therefore, the changes of T-SOD activity might be related to the oxidative damage's intensity and time, which is higher at slight or transitory damage, opposite lower.…”

Section: Discussionmentioning

confidence: 89%

Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks

Xiong

Xing

et al. 2021

Front. Vet. Sci.

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Vanadium (V) is an ultra-trace element presenting in humans and animals, but excessive V can cause toxic effects. Mitochondrial quality control (MQC) is an essential process for maintaining mitochondrial functions, but the relationship between V toxicity and MQC is unclear. To investigate the effects of excessive V on oxidative stress and MQC in duck hearts, 72 ducks were randomly divided into two groups, including the control group and the V group (30 mg of V/kg dry matter). The cardiac tissues were collected for the histomorphology observation and oxidative stress status evaluation at 22 and 44 days. In addition, the mRNA and protein levels of MQC-related factors were also analyzed. The results showed that excessive V could trigger vacuolar degeneration, granular degeneration, as well as mitochondrial vacuolization and swelling in myocardial cells. In addition, CAT activity was elevated in two time points, while T-SOD activity was increased in 22 days but decreased in 44 days after V treatment. Meanwhile, excessive V intake could also increase the number of Drp1 puncta, the mRNA levels of mitochondrial fission–related factors (Drp1and MFF), and protein (MFF) level, but decrease the number of Parkin puncta and the mitochondrial biogenesis (PGC-1α, NRF-1, and TFAM), mitochondrial fusion (OPA1, Mfn1, and Mfn2), and mitophagy (Parkin, PINK1, P62, and LC3B) related mRNA levels and protein (PGC-1α, Mfn1, Mfn2, PINK1) levels. Collectively, our results suggested that excessive V could induce oxidative stress and MQC disorder in the heart of ducks.

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“…Similar to this study, refs. [47] and [48] suggested that Cd toxicity induces an elevation in SOD and CAT activities in the liver of zebra blenny, Salaria basilisca, and in the gills of the rare minnow Gobiocypris rarus. They suggested that the elevated SOD and CAT activities were responses to the excessive generation of ROS triggered by Cd toxicity, and [32] suggested that Cd toxicity significantly increases SOD and CAT activities in the liver and gills of P. olivaceus.…”

Section: Discussionmentioning

confidence: 99%

Toxic Impact of Dietary Cadmium on Bioaccumulation, Growth, Hematological Parameters, Plasma Components, and Antioxidant Responses in Starry Flounder (Platichthys stellatus)

Jung,

Kim,

Kang

2024

Fishes

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Starry flounders (Platichthys stellatus) (average weight—114.69 ± 13 g, length—19.0 ± 0.21 cm) underwent a 4-week exposure to varying dietary cadmium (Cd) concentrations: 0, 10, 20, 40, 60, and 80 mg/kg. Bioaccumulation patterns were revealed in the intestine, kidney, liver, and gills in descending order of accumulation. Significant declines in growth indicators—body weight gain (BWG), specific growth rate (SGR), and feed efficiency ratio percentage (FER)—were evident at 80 mg Cd/kg for 2 weeks and beyond 60 mg Cd/kg for 4 weeks. Hematological parameters, including red blood cell (RBC) count, hematocrit (Ht), and hemoglobin (Hb), remarkably reduced at 80 mg Cd/kg for both 2 and 4 weeks. The total plasma protein reduced significantly after exposure to Cd for 2 and 4 weeks, alongside increased glucose levels, glutamic oxaloacetic transaminase (GOT), and glutamic pyruvate transaminase (GPT). Considerable increases in antioxidant responses—superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione (GSH) levels—were observed after exposure to Cd for 2 and 4 weeks. P. stellatus exhibits a high accumulation of dietary Cd in specific tissues. Moreover, concentrations above 60 mg Cd/kg adversely affected the growth performance, hematological parameters, plasma components, and antioxidant responses.

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Single and combined exposures of waterborne Cu and Cd induced oxidative stress responses and tissue injury in female rare minnow (Gobiocypris rarus)

Cited by 21 publications

References 58 publications

miR-489-3p Regulates the Oxidative Stress Response in the Liver and Gill Tissues of Hybrid Yellow Catfish (Pelteobagrus fulvidraco♀ × P. vachelli♂) Under Cu2+ Exposure by Targeting Cu/Zn-SOD

miR-489-3p Regulates the Oxidative Stress Response in the Liver and Gill Tissues of Hybrid Yellow Catfish (Pelteobagrus fulvidraco♀ × P. vachelli♂) Under Cu2+ Exposure by Targeting Cu/Zn-SOD

Vanadium Induces Oxidative Stress and Mitochondrial Quality Control Disorder in the Heart of Ducks

Toxic Impact of Dietary Cadmium on Bioaccumulation, Growth, Hematological Parameters, Plasma Components, and Antioxidant Responses in Starry Flounder (Platichthys stellatus)

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