Single-cell analysis of bacteria by Raman microscopy: spectral information on the chemical composition of cells and on the heterogeneity in a culture

Schuster, K.C.; Urlaub, E.; Gapes, J. R.

doi:10.1016/s0167-7012(00)00169-x

Cited by 273 publications

(225 citation statements)

References 20 publications

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“…Raman bands characteristic of phospholipids are observed at 1655 cm 1 (cis-C C stretch) 9 and 716 cm 1 (head group). 9 Raman bands ascribable to carbohydrates 7,8,10 are observed around 1030-1130 cm 1 . Spectra measured at 11 and 31 min show a strong peak at 1602 cm 1 .…”

Section: Resultsmentioning

confidence: 98%

“…The amide I band 3,4,7,8 of proteins is observed at 1658 cm 1 , indicating that the˛-helix secondary structure is dominant in the proteins existing in the cell. The strong 1446 cm 1 band is ascribed to the C-H bending 3,4,7,8 modes including CH 2 scissors 9 and CH 3 degenerate deformation. 9 The 1300 cm 1 band is assigned to the CH 2 twisting mode.…”

Section: Resultsmentioning

confidence: 99%

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Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy

Huang

Karashima

Yamamoto

et al. 2002

J Raman Spectroscopy

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The process of eukaryotic cell division has been studied at the molecular level by Raman spectroscopy. Time-and space-resolved Raman spectra of a living fission yeast cell were successfully recorded with a 100 s time resolution and a 250 nm spatial resolution by using a confocal multi-channel Raman microspectrometer. Raman spectra characteristic of the nucleus, mitochondrion and septum were identified and time-dependent changes of major molecular components during the cell cycle were elucidated.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy

Huang

Karashima

Yamamoto

et al. 2002

J Raman Spectroscopy

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“…Recent literature shows that Raman spectroscopic imaging is emerging as a key technology for single-cell analysis, including in vivo lipidomics [19], chemical composition of bacterial cells [20], lignin in plant cell walls [21], and metabolism in combination with stable isotope incorporation [22][23][24]. Researchers have also begun capitalizing on the speciic beneits of resonance Raman spectroscopic imaging to localize carotenoids in individual cells and tissues.…”

Section: Introductionmentioning

confidence: 99%

Localizing and Quantifying Carotenoids in Intact Cells and Tissues

Timlin¹,

Collins²,

Beechem³

et al. 2017

Carotenoids

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Raman spectroscopy provides detailed information about the molecular structure of carotenoids. Advances in detector sensitivity and acquisition speed have driven the expansion of Raman spectroscopy from a bulk analytical tool to a powerful method for mapping carotenoid abundance in cells and tissues. In many applications, the technique is compatible with living organisms, providing highly speciic molecular structure information in intact cells and tissues with subcellular spatial resolution. This leads to spatial-temporalchemical resolution critical to understanding the complex processes in the life cycle of carotenoids and other biomolecules.

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“…It completely overcomes the shortcoming of Raman small cross sections. SERS is capable to characterize [7][8][9][10], identify [11,12], and differentiate [13,14] pathogenic microorganisms in synergy with chemometrics, based on the biochemical, chemical, and their structural properties.…”

Section: Sers Effectmentioning

confidence: 99%

The Intricate Nature of SERS: Real‐Life Applications and Challenges

Dina¹,

Colniță²

2017

Raman Spectroscopy and Applications

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Testing for the presence of microorganisms in biological samples in order to diagnose infections is very common at all levels of health care. There is a growing need to ensure appropriate diagnosis by also minimizing the analysis time, both being very important concerns related to the risk of developing an antimicrobial resistance. Moreover, there are important medical and financial implications associated with infections. In this chapter, we will discuss the latest ultrasensitive and selective, but simple, rapid and inexpensive bacteria detection and identification methods by using receptor-free and innovative immobilization principles of the biomass. Raman spectroscopy, which combines the selectivity of the method with the sensitivity of the surface-enhanced Raman scattering (SERS) effect, is used in correlation with chemometric techniques in order to develop biosensors for pathogenic microorganisms.

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Single-cell analysis of bacteria by Raman microscopy: spectral information on the chemical composition of cells and on the heterogeneity in a culture

Cited by 273 publications

References 20 publications

Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy

Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy

Localizing and Quantifying Carotenoids in Intact Cells and Tissues

The Intricate Nature of SERS: Real‐Life Applications and Challenges

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