Single-cell cloning and expansion of human induced pluripotent stem cells by a microfluidic culture device

Matsumura, Takuya; Tatsumi, Kazuhiro; Noda, Yuichiro; Nakanishi, Naoyuki; Okonogi, Atsuhito; Hirano, Kunio; Li, Liu; Osumi, Takashi; Tada, Takashi; Kotera, Hidetoshi

doi:10.1016/j.bbrc.2014.09.081

Cited by 17 publications

(12 citation statements)

References 20 publications

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“…Meanwhile, interactions between cells also can be simulated. In recent years, microfluidic cell culture equipment has been used in tissue engineering, diagnostics, drug screening, immunology, cancer research, and stem cells proliferation and differentiation . Taylor introduced the first microfluidic system for complete mammalian cell culture.…”

Section: Advanced Microfluidic Applicationsmentioning

confidence: 99%

Advances in Microfluidics Applied to Single Cell Operation

Zhu

Chu

Wang

2018

Biotechnology Journal

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The field of microbiology have traditionally been concerned with and focused on studies at the population level. Microfluidic platforms have emerged as important tools for biology research at a small scale, even down to a single cell level. The spatial and temporal control of cells and stimuli transported by microfluidic channels in well-designed microsystems realized the studies of specific cells in a controlled microenvironment. The true cellular physiology responses, which are obtained mostly by inference from population-level data, could be revealed in this way. Nowadays, significant applications like cell culture, analysis, sorting, genomics, and proteomics at the single cell level have been achieved in microfluidic chips. Highly integrated microfluidic systems with complete bio-analytic functions are also coming forth and of great promise for single cell related physiology, biomedical, and high throughput screening research. Herein, the leads of technologies applied to single cell operation are reviewed. Challenges and potentials of these works are also summarized, to highlight fields for further research.

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Section: Advanced Microfluidic Applicationsmentioning

confidence: 99%

Advances in Microfluidics Applied to Single Cell Operation

Zhu

Chu

Wang

2018

Biotechnology Journal

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“…The study reported that hiPSCs did not show any signs of chromosomal abnormalities and maintained genetic characteristics of the parental lineage. [71] The abovementioned studies demonstrated that flow-based systems facilitate tight control over cellular environment, permitting mechanistic studies of stem cell renewal and differentiation.…”

Section: Microfluidic Devices To Mimic Stem Cell Microenvironmentmentioning

confidence: 99%

In Vitro Microscale Models for Embryogenesis

Rico-Varela

Wan

2018

Adv. Biosys.

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Embryogenesis is a highly regulated developmental process requiring complex mechanical and biochemical microenvironments to give rise to a fully developed and functional embryo. Significant efforts have been taken to recapitulate specific features of embryogenesis by presenting the cells with developmentally relevant signals. The outcomes, however, are limited partly due to the complexity of this biological process. Microtechnologies such as micropatterned and microfluidic systems, along with new emerging embryonic stem cell-based models, could potentially serve as powerful tools to study embryogenesis. The aim of this article is to review major studies involving the culturing of pluripotent stem cells using different geometrical patterns, microfluidic platforms, and embryo/embryoid body-on-a-chip modalities. Indeed, new research opportunities have emerged for establishing in vitro culture for studying human embryogenesis and for high-throughput pharmacological testing platforms and disease models to prevent defects in early stages of human development.

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“…These fabricated traps enhance medium circulation in the device and mimic the blood circulation. Mehta et al [28] fabricated two different types of traps in the device for bubbles trapping with advantages of simple, stable as well as no intervention with fluid flow. Bubbles catching was executed by the addition of a little tube-shaped chamber at the association of inlet and microchannel.…”

Section: Bubblementioning

confidence: 99%

Microfluidics Engineering: Recent Trends, Valorization, and Applications

2017

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In recent years, microfluidics engineering has gained increasing research interests in assimilation and designing novel constructs for various application in bio-and non-bio sectors of the modern world. Soft and photo-lithographic fabricated devices have a potential to control the diffusion and flow of liquids in channels. The fabricated microfluidic devices of unique geometry facilitate excellent control over extracellular microenvironment and soluble factor interactions. The current devices do not only replace the in-vitro approaches but also provide a new understanding of biomolecules separation and flow in the network of microchannels. The appropriate physiological responses of cells in biological studies or drug screening analysis require engineering of active cells outside the body to enhance their maximum potential for best cellular therapy. This review work mainly focuses on the current methodologies involved in the fabrication process and limitations of cell array technology. The information is given on considerable advantages of microfluidic devices. Towards the end, potential applications covering microfluidic-based in-vitro drug analysis and analytical-based separation/detachment are discussed with potential future viewpoints.

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Single-cell cloning and expansion of human induced pluripotent stem cells by a microfluidic culture device

Cited by 17 publications

References 20 publications

Advances in Microfluidics Applied to Single Cell Operation

Advances in Microfluidics Applied to Single Cell Operation

In Vitro Microscale Models for Embryogenesis

Microfluidics Engineering: Recent Trends, Valorization, and Applications

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