Single-cell immune profiling reveals long-term changes in myeloid cells and identifies a novel subset of CD9+ monocytes associated with COVID-19 hospitalization

Pandori, William; Padgett, Lindsey E.; Alimadadi, Ahmad; Gutierrez, Norma; Araujo, Daniel J.; Huh, Christine J.; Olingy, Claire; Dinh, Huy Q.; Wu, Runpei; Vijayanand, Pandurangan; Chee, Serena; Ottensmeier, Christian H.; Hedrick, Catherine C.

doi:10.1002/jlb.4cova0122-076r

Cited by 7 publications

(3 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While no difference in major inflammatory cytokines between the three groups was observed, correlations of CD169 + monocyte subsets with cytokines suggested that specific activated monocyte subsets produce high levels of proinflammatory cytokines in PPASC. Correlations of D-dimer with CD169 + non-classical monocytes observed herein were corroborated by the findings of Pandori et al (2022) in a cohort of individuals hospitalized for COVID-19 38 . Interestingly, their cohort did not display the increases in total monocyte populations in their hospitalized group but displayed decreases in non-classical monocyte percentages and steady levels of classical and intermediate monocyte percentages from total CD45 + cells in participants hospitalized for COVID-19 up to 90 days following admission.…”

Section: Discussionsupporting

confidence: 91%

Elevated circulating monocytes and monocyte activation in pulmonary post-acute sequelae of SARS-CoV-2 infection

Park

Dean

Jiyarom

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Monocytes and macrophages play a pivotal role in inflammation during acute SARS-CoV-2 infection. However, their contribution to the development of post-acute sequelae of SARS-CoV-2 infection (PASC) are not fully elucidated. Methods: A cross sectional study was conducted comparing plasma cytokine and monocyte levels among three groups: participants with pulmonary PASC (PPASC) with a reduced predicted diffusing capacity for carbon monoxide [DLCOc, <80%; (PG)]; fully recovered from SARS-CoV-2 with no residual symptoms (recovered group, RG); and negative for SARS-CoV-2 (negative group, NG). The expressions of cytokines were measured in plasma of study cohort by Luminex assay. The percentages and numbers of monocyte subsets (classical, intermediate, and non-classical monocytes) and monocyte activation (defined by CD169 expression) were analyzed using flow cytometry analysis of peripheral blood mononuclear cells. Results: Plasma IL-1Rα levels were elevated but FGF levels were reduced in PG compared to NG. Circulating monocytes and three subsets were significantly higher in PG and RG compared to NG. PG and RG exhibited higher levels of CD169+ monocyte counts and higher CD169 expression was detected in intermediate and non-classical monocytes from RG and PG than that found in NG. Further correlation analysis with CD169+ monocyte subsets revealed that CD169+ intermediate monocytes negatively correlated with DLCOc%, and CD169+ non-classical monocytes positively correlated with IL-1α, IL-1β, MIP-1α, Eotaxin, and IFN-γ. Conclusion: This study present evidence that COVID convalescents exhibit monocyte alteration beyond the acute COVID-19 infection period even in convalescents with no residual symptoms. These data provide further rational for determining the role of monocyte subsets in PPASC pathogenesis.

show abstract

Section: Discussionsupporting

confidence: 91%

Elevated circulating monocytes and monocyte activation in pulmonary post-acute sequelae of SARS-CoV-2 infection

Park

Dean

Jiyarom

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Correlations of D-dimer with CD169 + non-classical monocytes observed herein were corroborated by the findings of Pandori et al. ( 39 ) in a cohort of individuals hospitalized for COVID-19 ( 39 ). Interestingly, their cohort did not display the increases in total monocyte populations in their hospitalized group but displayed decreases in non-classical monocyte percentages and steady levels of classical and intermediate monocyte percentages from total CD45 + cells in participants hospitalized for COVID-19 up to 90 days following admission.…”

Section: Discussionsupporting

confidence: 90%

Elevated circulating monocytes and monocyte activation in COVID-19 convalescent individuals

et al. 2023

View full text Add to dashboard Cite

BackgroundMonocytes and macrophages play a pivotal role in inflammation during acute SARS-CoV-2 infection. However, their contribution to the development of post-acute sequelae of SARS-CoV-2 infection (PASC) are not fully elucidated.MethodsA cross-sectional study was conducted comparing plasma cytokine and monocyte levels among three groups: participants with pulmonary PASC (PPASC) with a reduced predicted diffusing capacity for carbon monoxide [DLCOc, <80%; (PG)]; fully recovered from SARS-CoV-2 with no residual symptoms (recovered group, RG); and negative for SARS-CoV-2 (negative group, NG). The expressions of cytokines were measured in plasma of study cohort by Luminex assay. The percentages and numbers of monocyte subsets (classical, intermediate, and non-classical monocytes) and monocyte activation (defined by CD169 expression) were analyzed using flow cytometry analysis of peripheral blood mononuclear cells.ResultsPlasma IL-1Ra levels were elevated but FGF levels were reduced in PG compared to NG. Circulating monocytes and three subsets were significantly higher in PG and RG compared to NG. PG and RG exhibited higher levels of CD169+ monocyte counts and higher CD169 expression was detected in intermediate and non-classical monocytes from RG and PG than that found in NG. Further correlation analysis with CD169+ monocyte subsets revealed that CD169+ intermediate monocytes negatively correlated with DLCOc%, and CD169+ non-classical monocytes positively correlated with IL-1α, IL-1β, MIP-1α, Eotaxin, and IFN-γ.ConclusionThis study present evidence that COVID convalescents exhibit monocyte alteration beyond the acute COVID-19 infection period even in convalescents with no residual symptoms. Further, the results suggest that monocyte alteration and increased activated monocyte subsets may impact pulmonary function in COVID-19 convalescents. This observation will aid in understanding the immunopathologic feature of pulmonary PASC development, resolution, and subsequent therapeutic interventions.

show abstract

“…At the same time, no institutions were founded for platelet-to-lymphocyte ratio (PLR). 17 , 18 , 20 , 21 , 23 – 27 …”

Section: Introductionmentioning

confidence: 99%

Correlations of Long COVID Symptoms and Inflammatory Markers of Complete Blood Count (CBC): A cross-sectional study.

Radkhah,

Omidali,

Hejrati

et al. 2023

Journal of Community Hospital Internal Medicine Perspectives

View full text Add to dashboard Cite

Background Long-COVID refers to lasting unspecific symptoms like fatigue, decreased concentration and sleep issues after infection which persist for at least three months and cannot be attributed to other causes. Previous studies surveyed the association between inflammatory markers like C - reactive protein (CRP) at hospital admission and long-COVID symptoms in the preceding months. Post-COVID syndrome can affect one-third of patients. Thus early diagnosis can assist in reducing burdens on public health. We attempted to see any correlations between complete blood count (CBC) markers (like red blood cell (RBC), white blood cell (WBC), Neutrophil to lymphocyte ratio (NLR), etc.) at hospital admission and long COVID symptoms at a 6-month follow-up. Methods 167 patients (44.9% females, mean age 49 years old) answered semi-structural interviews through telemedicine which focused on the three prominent symptoms: fatigue, loss of concentration and decreased libido. Results : Two third of patients have symptoms of long COVID and others do not have. NLR in the symptomatic group was statically higher. Patients who underwent decreased libido at a 6-month follow-up had significantly more severe lymphopenia (p = 0.028) and higher NLR values (p-value = 0.007). Poor mental concentration is associated with high WBC in numbers and polymorphonuclear (PMN) count. Other symptoms do not correlate with blood markers. Conclusion Utilizing available data like CBC can help predict the upcoming symptoms of previously hospitalized patients and further measures like rehabilitation. Additional investigations should be done on the effect of COVID vaccination on converting long COVID. Different variants of the virus may have different results.

show abstract

Single-cell immune profiling reveals long-term changes in myeloid cells and identifies a novel subset of CD9+ monocytes associated with COVID-19 hospitalization

Cited by 7 publications

References 42 publications

Elevated circulating monocytes and monocyte activation in pulmonary post-acute sequelae of SARS-CoV-2 infection

Elevated circulating monocytes and monocyte activation in pulmonary post-acute sequelae of SARS-CoV-2 infection

Elevated circulating monocytes and monocyte activation in COVID-19 convalescent individuals

Correlations of Long COVID Symptoms and Inflammatory Markers of Complete Blood Count (CBC): A cross-sectional study.

Contact Info

Product

Resources

About