Single cell level detection of Escherichia coli in microfluidic device

Smartphone-based, sensitive µPAD detection of urinary tract infection and gonorrhea

“…This is still possible since we are detecting antigens from bacteria but not the viable cell colonies. We have previously addressed this sub-singlecell level detection with particle immunoagglutination assays, most notably in Han et al (2008). Our assay is also superior in detecting UTI, with a superior detection limit (10 CFU/mL) and antibody-based specificity, which is not possible with the currently available nitrite strips.…”

Miniaturized Detection Systems

“…one not integrated on-chip) optical fiber. 79 Single-cell detection per microdevice was reported which increased to 4 cfu upon the addition of an extra washing step to determine only viable bacteria. While the volume processed by each device was 100 µL, later work by the authors connected the microfluidic system to a larger piped network, sampling a fraction of the water supply and achieving a 10 cfu mL −1 LOD in less than 5 min.…”

Technological Development of Antibody Immobilization for Optical Immunoassays: Progress and Prospects

“…This sensor was fabricated by electrodepositing AuNPs (gold nanoparticles) on the glassy carbon electrode surface to promote electron transfer, then L-cysteine and another layer of AuNPs were assembled onto the modified electrode surface to amplify the ECL signal of peroxydisulfate, and the antibody was immobilized through the electrostatic interaction between AuNPs and antibody (Wang et al, 2012). The conjugation of antibody to microparticles and thin-film structures has also been reported in recent years (Cooper et al, 2011;Han et al, 2008). Recently, a fiber-optic localized surface plasmon resonance sensor was fabricated by Jeong et al (2013) using AuNPs on Scheme 6.…”