Single-Cell MALDI-MS as an Analytical Tool for Studying Intrapopulation Metabolic Heterogeneity of Unicellular Organisms

Amantonico, Andrea; Urban, Pawel L.; Fagerer, Stephan R.; Balabin, Roman M.; Zenobi, Renato

doi:10.1021/ac1015326

Cited by 140 publications

(112 citation statements)

References 48 publications

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“…Hence, a key step to understanding the evolution of microbial markets is the notion of individual variation in partner quality. Advances in the study of microbial individuality have revealed that the typical population-level focus in microbiology can mask the existence of different phenotypes in microbial communities (99,100). Individuality (i.e., cell-to-cell phenotypic variability) of bacteria is an important factor driving microbial ecology and evolution (101,102).…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

Evolution of microbial markets

Werner

Strassmann

Ivens

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

191

139

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Biological market theory has been used successfully to explain cooperative behavior in many animal species. Microbes also engage in cooperative behaviors, both with hosts and other microbes, that can be described in economic terms. However, a market approach is not traditionally used to analyze these interactions. Here, we extend the biological market framework to ask whether this theory is of use to evolutionary biologists studying microbes. We consider six economic strategies used by microbes to optimize their success in markets. We argue that an economic market framework is a useful tool to generate specific and interesting predictions about microbial interactions, including the evolution of partner discrimination, hoarding strategies, specialized versus diversified mutualistic services, and the role of spatial structures, such as flocks and consortia. There is untapped potential for studying the evolutionary dynamics of microbial systems. Market theory can help structure this potential by characterizing strategic investment of microbes across a diversity of conditions. cooperation | mutualism | trade | partner choice

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Section: Discussion and Perspectivesmentioning

confidence: 99%

Evolution of microbial markets

Werner

Strassmann

Ivens

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

191

139

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“…This method allowed the identification of several metabolites (e.g., three phenylphenalenones) from specialized cells. Amantonico et al (6) studied the profile of selected metabolites (ADP, ATP, GTP and UDP-glucose) in single cells of Closterium acerosum using negative-mode MALDI-MS. In an earlier example, the hypothesis that intercellular transport of carbon occurs by diffusion during photosynthesis in C-4 plants was put to a test.…”

mentioning

confidence: 99%

High-resolution metabolic mapping of cell types in plant roots

Moussaieff

Rogachev

Brodsky

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

132

105

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Metabolite composition offers a powerful tool for understanding gene function and regulatory processes. However, metabolomics studies on multicellular organisms have thus far been performed primarily on whole organisms, organs, or cell lines, losing information about individual cell types within a tissue. With the goal of profiling metabolite content in different cell populations within an organ, we used FACS to dissect GFP-marked cells from Arabidopsis roots for metabolomics analysis. Here, we present the metabolic profiles obtained from five GFP-tagged lines representing core cell types in the root. Fifty metabolites were putatively identified, with the most prominent groups being glucosinolates, phenylpropanoids, and dipeptides, the latter of which is not yet explored in roots. The mRNA expression of enzymes or regulators in the corresponding biosynthetic pathways was compared with the relative metabolite abundance. Positive correlations suggest that the rate-limiting steps in biosynthesis of glucosinolates in the root are oxidative modifications of side chains. The current study presents a work flow for metabolomics analyses of cell-type populations.

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“…In addition to the MSI experiments, it is capable of conducting near real-time in-situ SCMS to elucidate detailed chemical information from live eukaryotic cells 16 , which is a major advantage compared with other vacuum based SCMS techniques (such as MALDI 10 and SIMS 21 ). The small size of the probe tip provides the ability to be inserted into a live eukaryotic cell and to extract and ionize the intracellular compounds for immediate MS analysis.…”

Section: Discussionmentioning

confidence: 99%

“…SCA enables the analysis of biological systems at a more fundamental level than traditional cell analysis techniques, which produce an averaged result of a population of cells, potentially providing insights that are previously intractable 9 . MS techniques have recently been applied to SCA (termed single cell mass spectrometry or SCMS) using non-ambient techniques such as MALDI MS 10 and ToF SIMS 11 in which cells are pretreated before analysis, and with ambient techniques such as LAESI MS 12 and direct extraction methods, such as live single-cell video-MS 13, 14 , to analyze a wide variety of cell types such as egg, plant, and cancer. Ambient techniques have the advantage of being applied to live cells, which again minimizes the artifacts, leading to a better representation of the metabolites in the live cells.…”

Section: Introductionmentioning

confidence: 99%

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Rao

Pan

Yang

2016

JoVE

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Mass spectrometry imaging (MSI) and in-situ single cell mass spectrometry (SCMS) analysis under ambient conditions are two emerging fields with great potential for the detailed mass spectrometry (MS) analysis of biomolecules from biological samples. The single-probe, a miniaturized device with integrated sampling and ionization capabilities, is capable of performing both ambient MSI and in-situ SCMS analysis. For ambient MSI, the single-probe uses surface micro-extraction to continually conduct MS analysis of the sample, and this technique allows the creation of MS images with high spatial resolution (8.5 μm) from biological samples such as mouse brain and kidney sections. Ambient MSI has the advantage that little to no sample preparation is needed before the analysis, which reduces the amount of potential artifacts present in data acquisition and allows a more representative analysis of the sample to be acquired. For in-situ SCMS, the single-probe tip can be directly inserted into live eukaryotic cells such as HeLa cells, due to the small sampling tip size (< 10 μm), and this technique is capable of detecting a wide range of metabolites inside individual cells at near real-time. SCMS enables a greater sensitivity and accuracy of chemical information to be acquired at the single cell level, which could improve our understanding of biological processes at a more fundamental level than previously possible. The single-probe device can be potentially coupled with a variety of mass spectrometers for broad ranges of MSI and SCMS studies.

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Single-Cell MALDI-MS as an Analytical Tool for Studying Intrapopulation Metabolic Heterogeneity of Unicellular Organisms

Cited by 140 publications

References 48 publications

Evolution of microbial markets

Evolution of microbial markets

High-resolution metabolic mapping of cell types in plant roots

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

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