Single cell model for re‐entrainment to a shifted light cycle

Beurden, Anouk W. van; Schoonderwoerd, Robin A.; Tersteeg, Mayke M. H.; Gutiérrez, Pablo de Torres; Michel, Stephan; Blommers, Ruben; Rohling, Jos H. T.; Meijer, Johanna H.

doi:10.1096/fj.202200478r

Cited by 5 publications

(1 citation statement)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent study on mice with imposed delays in the LD cycle investigated Per2 expression at a single-cell level throughout the SCN. The ventral part contains about 40% fast phase-shifting and 60% slow phase-shifting neurons while the dorsal SCN almost exclusively comprises slow-shifting neurons ( van Beurden et al , 2022 ).…”

Section: Intra-tissue Desynchronymentioning

confidence: 99%

Different levels of circadian (de)synchrony – where does it hurt?

et al. 2023

View full text Add to dashboard Cite

A network of cellular timers ensures the maintenance of homeostasis by temporal modulation of physiological processes across the day. These so-called circadian clocks are synchronized to geophysical time by external time cues (or zeitgebers). In modern societies, natural environmental cycles are disrupted by artificial lighting, around-the-clock availability of food or shift work. Such contradictory zeitgeber input promotes chronodisruption, i.e., the perturbation of internal circadian rhythms, resulting in adverse health outcomes. While this phenomenon is well described, it is still poorly understood at which level of organization perturbed rhythms impact on health and wellbeing. In this review, we discuss different levels of chronodisruption and what is known about their health effects. We summarize the results of disrupted phase coherence between external and internal time vs. misalignment of tissue clocks amongst each other, i.e., internal desynchrony. Last, phase incoherence can also occur at the tissue level itself. Here, alterations in phase coordination can emerge between cellular clocks of the same tissue or between different clock genes within the single cell. A better understanding of the mechanisms of circadian misalignment and its effects on physiology will help to find effective tools to prevent or treat disorders arising from modern-day chronodisruptive environments.

show abstract

Section: Intra-tissue Desynchronymentioning

confidence: 99%

Different levels of circadian (de)synchrony – where does it hurt?

et al. 2023

View full text Add to dashboard Cite

show abstract

Single cell model for re‐entrainment to a shifted light cycle

et al. 2022

Self Cite

View full text Add to dashboard Cite

Our daily 24‐h rhythm is synchronized to the external light–dark cycle resulting from the Earth's daily rotation. In the mammalian brain, the suprachiasmatic nucleus (SCN) serves as the master clock and receives light‐mediated input via the retinohypothalamic tract. Abrupt changes in the timing of the light–dark cycle (e.g., due to jet lag) cause a phase shift in the circadian rhythms in the SCN. Here, we investigated the effects of a 6‐h delay in the light–dark cycle on PERIOD2::LUCIFERASE expression at the single‐cell level in mouse SCN organotypic explants. The ensemble pattern in phase shift response obtained from individual neurons in the anterior and central SCN revealed a bimodal distribution; specifically, neurons in the ventrolateral SCN responded with a rapid phase shift, while neurons in the dorsal SCN generally did not respond to the shift in the light–dark cycle. We also stimulated the hypothalamic tract in acute SCN slices to simulate light‐mediated input to the SCN; interestingly, we found similarities between the distribution and fraction of rapid shifting neurons (in response to the delay) and neurons that were excited in response to electrical stimulation. These results suggest that a subpopulation of neurons in the ventral SCN that have an excitatory response to light input, shift their clock more readily than dorsal located neurons, and initiate the SCN's entrainment to the new light–dark cycle. Thus, we propose that light‐excited neurons in the anterior and central SCN play an important role in the organism's ability to adjust to changes in the external light–dark cycle.

show abstract

Glaucoma‐inducing retinal ganglion cell degeneration alters diurnal rhythm of key molecular components of the central clock and locomotor activity in mice

Barsanele,

de Assis,

da Silva

et al. 2024

The FASEB Journal

View full text Add to dashboard Cite

Glaucoma is a chronic optic neuropathy characterized by the progressive degeneration of retinal ganglion cells (RGC). These cells play a crucial role in transmitting visual and non‐visual information to brain regions, including the suprachiasmatic nucleus (SCN), responsible for synchronizing biological rhythms. To understand how glaucoma affects circadian rhythm synchronization, we investigated potential changes in the molecular clock machinery in the SCN. We found that the progressive increase in intraocular pressure (IOP) negatively correlated with spontaneous locomotor activity (SLA). Transcriptome analysis revealed significant alterations in the SCN of glaucomatous mice, including downregulation of genes associated with circadian rhythms. In fact, we showed a loss of diurnal oscillation in the expression of vasoactive intestinal peptide (Vip), its receptor (Vipr2), and period 1 (Per1) in the SCN of glaucomatous mice. These findings were supported by the 7‐h phase shift in the peak expression of arginine vasopressin (Avp) in the SCN of mice with glaucoma. Despite maintaining a 24‐h period under both light/dark (LD) and constant dark (DD) conditions, glaucomatous mice exhibited altered SLA rhythms, characterized by decreased amplitude. Taken altogether, our findings provide evidence of how glaucoma affects the regulation of the central circadian clock and its consequence on the regulation of circadian rhythms.

show abstract

Single cell model for re‐entrainment to a shifted light cycle

Cited by 5 publications

References 39 publications

Different levels of circadian (de)synchrony – where does it hurt?

Different levels of circadian (de)synchrony – where does it hurt?

Single cell model for re‐entrainment to a shifted light cycle

Glaucoma‐inducing retinal ganglion cell degeneration alters diurnal rhythm of key molecular components of the central clock and locomotor activity in mice

Contact Info

Product

Resources

About

Single cell model for re‐entrainment to a shifted light cycle

Cited by 5 publications

References 39 publications

Different levels of circadian (de)synchrony ­– where does it hurt?

Different levels of circadian (de)synchrony ­– where does it hurt?

Single cell model for re‐entrainment to a shifted light cycle

Glaucoma‐inducing retinal ganglion cell degeneration alters diurnal rhythm of key molecular components of the central clock and locomotor activity in mice

Contact Info

Product

Resources

About

Different levels of circadian (de)synchrony – where does it hurt?

Different levels of circadian (de)synchrony – where does it hurt?