Single-cell printing to form three-dimensional lines of olfactory ensheathing cells

Othon, Christina M.; Wu, Xingjia; Anders, Juanita J.; Ringeisen, Bradley R.

doi:10.1088/1748-6041/3/3/034101

Cited by 90 publications

(51 citation statements)

References 24 publications

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“…At the same time, this group developed the improved BioLP™ approach in an attempt to limit direct interaction between the laser and sensitive biomaterials (Barron et al 2004a). This technique also proved capable of depositing highly viable human osteosarcoma cells (Barron et al 2005) and olfactory ensheating cells (Othon et al 2008) onto Matrigel™ substrates.…”

Section: Current Progress Chrisey Et Al First Demonstrated the Maplementioning

confidence: 98%

Biofabrication: an overview of the approaches used for printing of living cells

Ferris

Gilmore

Wallace

et al. 2013

Appl Microbiol Biotechnol

210

132

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The development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable and reproducible printing of cells. This review outlines the general principles and current progress and compares the advantages and challenges for the most widely used biofabrication techniques for printing cells: extrusion, laser, microvalve, inkjet and tissue fragment printing. It is expected that significant advances in cell printing will result from synergistic combinations of these techniques and lead to optimised resolution, throughput and the overall complexity of printed constructs. AbstractThe development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable, reproducible printing of cells. This review outlines the general principles, current progress, and compares the advantages and challenges for the most widely used biofabrication techniques for printing cells: extrusion, laser, microvalve, inkjet and tissue fragment printing. It is expected that significant advances in cell printing will result from synergistic combinations of these techniques and lead to optimised resolution, throughput and the overall complexity of printed constructs.

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Section: Current Progress Chrisey Et Al First Demonstrated the Maplementioning

confidence: 98%

Biofabrication: an overview of the approaches used for printing of living cells

Ferris

Gilmore

Wallace

et al. 2013

Appl Microbiol Biotechnol

210

132

View full text Add to dashboard Cite

show abstract

“…During and after deposition of the 3D scaffold the hydrogel is gelled by thermal-, photo-, or chemical-based approaches. [82,94,113,121,138] The use of toxic materials and high energies requires special care to leave the cells viable and intact. After printing, the tissue/organ construct is matured, either by seeding of cells onto the artificial scaffold or cultivation of cell-laden scaffolds, until proper function can be assessed.…”

Section: D Bioprinted Organ Modelsmentioning

confidence: 99%

“…In addition, these cell-printed scaffolds may provide an alternative for spinal cord repair studies, as the single-cell www.advancedsciencenews.com www.advhealthmat.de patterns formed here are on relevant size scales for neurite outgrowth. [121] …”

Section: Bioprinted Neural Tissuementioning

confidence: 99%

3D Miniaturization of Human Organs for Drug Discovery

Park

Wetzel

Dréau

et al. 2017

Adv Healthcare Materials

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“Engineered human organs” hold promises for predicting the effectiveness and accuracy of drug responses while reducing cost, time, and failure rates in clinical trials. Multiorgan human models utilize many aspects of currently available technologies including self‐organized spherical 3D human organoids, microfabricated 3D human organ chips, and 3D bioprinted human organ constructs to mimic key structural and functional properties of human organs. They enable precise control of multicellular activities, extracellular matrix (ECM) compositions, spatial distributions of cells, architectural organizations of ECM, and environmental cues. Thus, engineered human organs can provide the microstructures and biological functions of target organs and advantageously substitute multiscaled drug‐testing platforms including the current in vitro molecular assays, cell platforms, and in vivo models. This review provides an overview of advanced innovative designs based on the three main technologies used for organ construction leading to single and multiorgan systems useable for drug development. Current technological challenges and future perspectives are also discussed.

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“…They are combined with different DRL materials, including metals (gold, silver, or titanium), polymers (triazene, polyethylene naphthalate, polyimide, or cyanoacrylate), or hydrogels (gelatin). Most groups using LaBP for printing biomaterials apply ultraviolet (UV) lasers with 3 to 30 nanoseconds pulse durations and 193-nm [1,2] , 248-nm [3,4] , 266-nm [5] , 337-nm [6] , or 355-nm [7,8] wavelengths.…”

Section: Introductionmentioning

confidence: 99%

Laser-assisted bioprinting at different wavelengths and pulse durations with a metal dynamic release layer: A parametric study

Koch

Brandt

Deiwick

et al. 2017

ijb

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For more than a decade, living cells and biomaterials (typically hydrogels) are printed via laser-assisted bioprinting. Often, a thin metal layer is applied as laser-absorbing material called dynamic release layer (DRL). This layer is vaporized by focused laser pulses generating vapor pressure that propels forward a coated biomaterial. Different lasers with laser wavelengths from 193 to 1064 nanometer have been used. As a metal DRL gold, silver, or titanium layers have been used. The applied laser pulse durations were usually in the nanosecond range from 1 to 30 ns. In addition, some studies with femtosecond lasers have been published. However, there are no studies on the effect of all these lasers parameters on bioprinting with a metal DRL, and on comparing different wavelengths and pulse durations -except one study comparing 500 femtosecond pulses with 15 ns pulses. In this paper, the effects of laser wavelength (355, 532, and 1064 nm) and laser pulse duration (in the range of 8 to 200 ns) are investigated. Furthermore, the effects of laser pulse energy, intensity, and focal spot size are studied. The printed droplet volume, hydrogel jet velocity, and cell viability are analyzed. Keywords: bioprinting, laser-assisted bioprinting, laser-induced forward transfer, laser absorption layer, laser parametric study *Correspondence to: Lothar Koch, Laser Zentrum Hannover e.V., Nanotechnology Department, Hollerithallee 8, 30419 Hannover, Germany; Email: l.koch@lzh.de

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Single-cell printing to form three-dimensional lines of olfactory ensheathing cells

Cited by 90 publications

References 24 publications

Biofabrication: an overview of the approaches used for printing of living cells

Biofabrication: an overview of the approaches used for printing of living cells

3D Miniaturization of Human Organs for Drug Discovery

Laser-assisted bioprinting at different wavelengths and pulse durations with a metal dynamic release layer: A parametric study

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