Single cell / real-time imaging of bactericidal effect on the nano-structural surface

Nakade, Kazuki; Jindai, Keisuke; Sagawa, Takashi; Kojima, Hiroaki; Shimizu, Tomohiro; Shingubara, Shoso; Ito, Tetsuo

doi:10.1016/j.matpr.2018.11.115

Cited by 4 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amidst the development of these hypotheses, it was experimentally established that the effusion of intracellular fluid does occur for Escherichia coli on nanostructured surfaces using fluorescent proteins . This important finding confirmed that rupture of the bacterial membrane is involved in the bactericidal action presented by the nanostructures.…”

Section: Mechanisms Revealed From the Experimental Approachesmentioning

confidence: 76%

Critical Review of Nanopillar-Based Mechanobactericidal Systems

Hawi

Goel

Kumar

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

The rise of multidrug-resistant bacteria is the biggest threat to human health globally, as described by the World Health Organization. Mechanobactericidal surfaces provide a sustainable approach to addressing this concern by eradicating pathogens, especially bacteria, “right-at-the-point” of contacting the surface. However, the lack of a “design to manufacture” approach due to our limited understanding of the mechanobactericidal mechanism has impeded engineering optimization to develop scalable exploitation routes in various healthcare applications. It can be argued that the reason, most particularly, is the limitations and uncertainties associated with the current instrumentation and simulation capabilities, which has led to several streams of test protocols. This review highlights the current understanding on the mechanobactericidal mechanisms in light of the contributing factors and various techniques that are used to postulate these mechanisms. The review offers a critique on the variations observed on how nanostructured surfaces found in the literature have been evaluated such that the test protocols and outcomes are incomparable. The review also shows a strong need for developing more accurate models of a bacterium because the currently reported experimental data are insufficient to develop bacterial material models (constitutive equations). The review also alludes to the scarcity of direct experimental evidence of the mechanobactericidal mechanism, suggesting a strong need for further in situ monitoring as a future research direction.

show abstract

Section: Mechanisms Revealed From the Experimental Approachesmentioning

confidence: 76%

Critical Review of Nanopillar-Based Mechanobactericidal Systems

Hawi

Goel

Kumar

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

show abstract

“…The penetration mechanism indicates that cell rupture occurs at the vertex in contact with the nanostructure, whereas the stretching mechanism indicates that cell rupture occurs in the suspended area between the nanopillars that is not in contact with the structure. The exudation of cell fluid in the bacteria also indirectly proves that the bacteria are killed during cell rupture [ 97 ]. In addition, several studies have reported that the cell wall of a bacterium repairs itself and maintains its integrity, vitality, and reproduction after being repeatedly penetrated by the sharp tip of an atomic force microscope [ 98 ].…”

Section: Microbicidal Surfacementioning

confidence: 99%

Nature-Inspired Surface Structures Design for Antimicrobial Applications

Lee

Hussein

Chang

et al. 2023

IJMS

View full text Add to dashboard Cite

Surface contamination by microorganisms such as viruses and bacteria may simultaneously aggravate the biofouling of surfaces and infection of wounds and promote cross-species transmission and the rapid evolution of microbes in emerging diseases. In addition, natural surface structures with unique anti-biofouling properties may be used as guide templates for the development of functional antimicrobial surfaces. Further, these structure-related antimicrobial surfaces can be categorized into microbicidal and anti-biofouling surfaces. This review introduces the recent advances in the development of microbicidal and anti-biofouling surfaces inspired by natural structures and discusses the related antimicrobial mechanisms, surface topography design, material application, manufacturing techniques, and antimicrobial efficiencies.

show abstract