Single-cell RNA-sequencing of human eosinophils in allergic inflammation in the esophagus

Ben-Baruch Morgenstern, Netali; Rochman, Mark; Kotliar, Michael; Dunn, Julia L.M.; Mack, Lydia; Besse, John; Natale, Mia A.; Klingler, Andrea M.; Felton, Jennifer M.; Caldwell, Julie M.; Barski, Artem; Rothenberg, Marc E.

doi:10.1016/j.jaci.2024.05.029

Cited by 2 publications

References 59 publications

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Eosinophils restrict CRC metastasis by inhibiting pro-tumorigenic SPP1⁺macrophage differentiation

Handler,

Raju,

Gurtner

et al. 2024

Preprint

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Eosinophils, traditionally associated with allergic responses, have emerged as critical immune modulators in colorectal cancer (CRC). Here, we reveal that eosinophils actively shape the tumor microenvironment and influence metastatic progression. Using comprehensive transcriptomics analysis of human CRC and a murine orthotopic tumor model, we identify a conserved tumor-specific eosinophil signature and activation profile. Despite their declining presence in advanced CRC, eosinophils suppress metastatic dissemination by counteracting the pro-tumorigenic functions of SPP1+macrophages – a subset linked to immune exclusion and tumor metastasis. Mechanistically, eosinophils respond to tumor-derived signals and inhibit macrophage differentiation into SPP1+cells. Eosinophil depletion exacerbates peritoneal tumor spread. These findings highlight the pivotal role of eosinophils in restraining late-stage CRC progression and unveil a novel eosinophil–macrophage axis as potential therapeutic targets.

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Eosinophils restrict CRC metastasis by inhibiting pro-tumorigenic SPP1⁺macrophage differentiation

Handler,

Raju,

Gurtner

et al. 2024

Preprint

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Single-Cell Analysis: A Method for In-Depth Phenotyping of Cells Involved in Asthma

Rodríguez-González,

Guillén-Sánchez,

del Pozo

et al. 2024

IJMS

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Asthma is a chronic inflammatory lung disease with high prevalence, making it one of the most common chronic conditions worldwide. Its pathophysiology is influenced by a range of genetic and environmental factors, resulting in a complex and heterogeneous disease profile. Asthma is primarily associated with a type 2 (T2) immune response, though non-T2 endotypes also contribute to disease pathology. Generally, asthma is characterized by the infiltration and activation of various cell types, including dendritic cells, eosinophils, innate lymphoid cells, lymphocytes, mast cells, and neutrophils, which participate in T1, T2, and T17 immune responses. Despite advances in understanding, many questions remain unresolved. Therefore, emerging omic techniques, such as single-cell RNA sequencing (scRNA-seq), offer novel insights into the underlying mechanisms of asthma and the roles of these immune cells. Recent scRNA-seq studies in asthma have identified multiple novel immune cell subtypes and clusters, suggesting their potential functions in disease pathology. The rapid advancement of scRNA-seq technology now enables in-depth investigation of individual cells within tissues, allowing for precise cell-type classification and detailed molecular profiling. Nonetheless, certain limitations persist, which require further refinement in future studies.

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Single-cell RNA-sequencing of human eosinophils in allergic inflammation in the esophagus

Cited by 2 publications

References 59 publications

Eosinophils restrict CRC metastasis by inhibiting pro-tumorigenic SPP1⁺macrophage differentiation

Eosinophils restrict CRC metastasis by inhibiting pro-tumorigenic SPP1⁺macrophage differentiation

Single-Cell Analysis: A Method for In-Depth Phenotyping of Cells Involved in Asthma

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Single-cell RNA-sequencing of human eosinophils in allergic inflammation in the esophagus

Cited by 2 publications

References 59 publications

Eosinophils restrict CRC metastasis by inhibiting pro-tumorigenic SPP1+macrophage differentiation

Eosinophils restrict CRC metastasis by inhibiting pro-tumorigenic SPP1+macrophage differentiation

Single-Cell Analysis: A Method for In-Depth Phenotyping of Cells Involved in Asthma

Contact Info

Product

Resources

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Eosinophils restrict CRC metastasis by inhibiting pro-tumorigenic SPP1⁺macrophage differentiation

Eosinophils restrict CRC metastasis by inhibiting pro-tumorigenic SPP1⁺macrophage differentiation