Single-cell spatiotemporal analysis reveals cell fates and functions of transplanted mesenchymal stromal cells during bone repair

Yang, Chengyu; Li, Zeshun; Liu, Yang; Hou, Runpeng; Lin, Minmin; Fu, Linhao; Wu, Decheng; Liu, Quanying; Li, Kai; Liu, Chao

doi:10.1016/j.stemcr.2022.08.008

Cited by 11 publications

(5 citation statements)

References 55 publications

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“…There is much evidence that transplanted MSCs not only prevent the death of specialized cells of the affected tissue [33], but also have the ability to interact with the resident stem cells of the affected tissue and attract them to the lesions [45]. The question of the interaction of transplanted MSCs with resident MSCs of cartilage tissue remains open, but given the potential importance of this interaction, it can be assumed that MSCs for transplantation should have significant paracrine potential and the ability to chemoattract.…”

Section: Results Discussionmentioning

confidence: 99%

Current problems and persprectives of the treatment of cartilage injuries using mesenchimal stem cells

Fishchenko¹,

Botsul²,

Shuvalova³

et al. 2022

Rep. of Vinnytsia Nation. Med. Univ.

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Annotation. Since standard methods of treatment of cartilage trauma and inflammatory diseases lack effectiveness, at present one of the most promising strategies is mesenchymal stem cell transplantation. Despite the successes in this field, many issues, such as the selection of the tissue-source of mesenchymal stem cells (MSCs) and their optimization or modification in culture in order to achieve the maximum therapeutic effect, remains an actual task for both global and domestic scientists. The purpose of the study is to prepare a review of literature sources for the period 2004-2022 (in the vast majority on publications of the last five years) and to systematize new data on the biological characteristics of MSCs from various tissue sources, which are used in strategies for the treatment of cartilage lesions tissues, to analyze existing problems and further prospects, as well as potential ways of optimizing MSC cultivation methods to increase their regenerative potential. A study of 49 sources that met the inclusion criteria was carried out. A significant variety of strategies for the use of MSCs in animal models and in clinical practice was revealed. The main mechanisms ensuring the therapeutic effect of MSCs have been determined and can be used as criteria for optimizing cellular material. However, it was found that the question of the effectiveness of the use of differentiated MSCs, the selection of the tissue-source of MSCs, and the specifics of the use of MSCs from neonatal sources remains open. Thus, the analysis of literary sources revealed further promising ways to improve the therapeutic efficiency of MSC grafting in a wide range of cartilaginous lesions. There is a need to research the effects of MSCs from neonatal tissues, and especially the umbilical cord. This will become possible due to experimental research that will be carried out in the future.

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Section: Results Discussionmentioning

confidence: 99%

Current problems and persprectives of the treatment of cartilage injuries using mesenchimal stem cells

Fishchenko¹,

Botsul²,

Shuvalova³

et al. 2022

Rep. of Vinnytsia Nation. Med. Univ.

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“…fNP-MSCs also survived in the defect throughout repair. While only a small portion of the transplanted cells underwent osteogenic differentiation (OSX+), a significant portion maintained their expression of MSC and skeletal stem cell markers (SCA-1 and PRRX1) [ 26 ]. Pitacco P et al, designed a new bone tissue engineering strategy of incorporating human BMMSCs into fibrin-based bioinks and then bioprinting these into PCL frameworks to produce mechanically reinforced constructs.…”

Section: Bone Marrow Mesenchymal Stem (Stromal) Cells (Bmmscs)mentioning

confidence: 99%

Stem Cells and Bone Tissue Engineering

Gao,

Ruzbarsky,

Layne

et al. 2024

Life

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Segmental bone defects that are caused by trauma, infection, tumor resection, or osteoporotic fractures present significant surgical treatment challenges. Host bone autograft is considered the gold standard for restoring function but comes with the cost of harvest site comorbidity. Allograft bone is a secondary option but has its own limitations in the incorporation with the host bone as well as its cost. Therefore, developing new bone tissue engineering strategies to treat bone defects is critically needed. In the past three decades, the use of stem cells that are delivered with different scaffolds or growth factors for bone tissue engineering has made tremendous progress. Many varieties of stem cells have been isolated from different tissues for use in bone tissue engineering. This review summarizes the progress in using different postnatal stem cells, including bone marrow mesenchymal stem cells, muscle-derived stem cells, adipose-derived stem cells, dental pulp stem cells/periodontal ligament stem cells, periosteum stem cells, umbilical cord-derived stem cells, peripheral blood stem cells, urine-derived stem cells, stem cells from apical papilla, and induced pluripotent stem cells, for bone tissue engineering and repair. This review also summarizes the progress using exosomes or extracellular vesicles that are delivered with various scaffolds for bone repair. The advantages and disadvantages of each type of stem cell are also discussed and explained in detail. It is hoped that in the future, these preclinical results will translate into new regenerative therapies for bone defect repair.

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“…The connectivity proteins or biomolecules modied on the surface of nanoparticles bind to the specic receptors on the cell surface to enter the cell, which realizes the specic biomarkers and uorescence imaging diagnosis of cells and living tissues, thus enabling dynamic tracking of the cell status. 92 For example, nanoparticles for labeling MSCs have SPIONs, uorescently labeled mesoporous silica nanoparticles, gold nanoparticles, or quantum dots et al 6,14,93 It has been shown that local implantation of uorescent nanoparticles (fNPs)-labeled MSCs on the periosteal side of tibial defects could promote tibial defect repair and increase the number of stem cell and vascular maturity in mice by NIR-II live imaging 94 (Fig. 2).…”

Section: Cell Labelingmentioning

confidence: 99%

“…It has been shown that local implantation of fluorescent nanoparticles (fNPs)-labeled MSCs on the periosteal side of tibial defects could promote tibial defect repair and increase the number of stem cell and vascular maturity in mice by NIR-II live imaging 94 ( Fig. 2 ).…”

Section: Applications Of Nanoparticles In Bone Regenerationmentioning

confidence: 99%