Single-cell transcriptome analysis reveals liver injury induced by glyphosate in mice

Wu, Jiangpeng; Sun, Xiaowei; Hong, Xiaoping; Xie, Lulin; Shi, Zixu; Zhao, Liang; Du, Qingfeng; Xiao, Wei; Sun, Jing; Wang, Jigang

doi:10.1186/s11658-023-00426-z

Cited by 12 publications

(2 citation statements)

References 84 publications

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“…Nonetheless, even within the same cell type, gene expression at the microscopic level exhibits certain dissimilarities. In contrast, scRNA-seq technology enables the amplification and sequencing of the complete transcriptome of a single cell, facilitating the identification of overall gene expression and distribution information in individual cells [ 34 ]. This approach accurately portrays cell heterogeneity and classifies previously rare cell subpopulations, greatly contributing to a deeper understanding of the transcriptional profiles of diverse macrophage populations and their functional disparities in disease models [ 35 ].…”

Section: Development and Classification Of Macrophagesmentioning

confidence: 99%

Macrophage-driven cardiac inflammation and healing: insights from homeostasis and myocardial infarction

Zuo,

Sun,

et al. 2023

Cell Mol Biol Lett

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Early and prompt reperfusion therapy has markedly improved the survival rates among patients enduring myocardial infarction (MI). Nonetheless, the resulting adverse remodeling and the subsequent onset of heart failure remain formidable clinical management challenges and represent a primary cause of disability in MI patients worldwide. Macrophages play a crucial role in immune system regulation and wield a profound influence over the inflammatory repair process following MI, thereby dictating the degree of myocardial injury and the subsequent pathological remodeling. Despite numerous previous biological studies that established the classical polarization model for macrophages, classifying them as either M1 pro-inflammatory or M2 pro-reparative macrophages, this simplistic categorization falls short of meeting the precision medicine standards, hindering the translational advancement of clinical research. Recently, advances in single-cell sequencing technology have facilitated a more profound exploration of macrophage heterogeneity and plasticity, opening avenues for the development of targeted interventions to address macrophage-related factors in the aftermath of MI. In this review, we provide a summary of macrophage origins, tissue distribution, classification, and surface markers. Furthermore, we delve into the multifaceted roles of macrophages in maintaining cardiac homeostasis and regulating inflammation during the post-MI period.

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Section: Development and Classification Of Macrophagesmentioning

confidence: 99%

Macrophage-driven cardiac inflammation and healing: insights from homeostasis and myocardial infarction

Zuo,

Sun,

et al. 2023

Cell Mol Biol Lett

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“…Therefore, it is difficult to provide complete information about tumour cells by traditional research methods alone. In recent years, the rapid development of single-cell omics has allowed us to understand the changes in a cell population, biochemical characteristics, and immune status of tumour tissues during disease progression [ 10 ]. In addition, single-cell RNA-sequencing (scRNA-seq) combined with various immunofluorescence techniques can analyze tumour cells at multiple levels and perspectives.…”

Section: Introductionmentioning

confidence: 99%

Single-cell omics traces the heterogeneity of prostate cancer cells and the tumor microenvironment

Liu

Gao

et al. 2023

Cell Mol Biol Lett

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Prostate cancer is one of the more heterogeneous tumour types. In recent years, with the rapid development of single-cell sequencing and spatial transcriptome technologies, researchers have gained a more intuitive and comprehensive understanding of the heterogeneity of prostate cancer. Tumour-associated epithelial cells; cancer-associated fibroblasts; the complexity of the immune microenvironment, and the heterogeneity of the spatial distribution of tumour cells and other cancer-promoting molecules play a crucial role in the growth, invasion, and metastasis of prostate cancer. Single-cell multi-omics biotechnology, especially single-cell transcriptome sequencing, reveals the expression level of single cells with higher resolution and finely dissects the molecular characteristics of different tumour cells. We reviewed the recent literature on prostate cancer cells, focusing on single-cell RNA sequencing. And we analysed the heterogeneity and spatial distribution differences of different tumour cell types. We discussed the impact of novel single-cell omics technologies, such as rich omics exploration strategies, multi-omics joint analysis modes, and deep learning models, on future prostate cancer research. In this review, we have constructed a comprehensive catalogue of single-cell omics studies in prostate cancer. This article aimed to provide a more thorough understanding of the diagnosis and treatment of prostate cancer. We summarised and proposed several key issues and directions on applying single-cell multi-omics and spatial transcriptomics to understand the heterogeneity of prostate cancer. Finally, we discussed single-cell omics trends and future directions in prostate cancer.

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A Single‐Cell Transcriptome Profiling of Triptolide‐Induced Nephrotoxicity in Mice

Wu,

Guo,

Xia

et al. 2024

Advanced Biology

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Triptolide (TP), an active component isolated from the traditional Chinese herb Tripterygium wilfordii Hook F (TWHF), shows great promise for treating inflammation‐related diseases. However, its potential nephrotoxic effects remain concerning. The mechanism underlying TP‐induced nephrotoxicity is inadequately elucidated, particularly at single‐cell resolution. Hence, single‐cell RNA sequencing (scRNA‐seq) of kidney tissues from control and TP‐treated mice is performed to generate a thorough description of the renal cell atlas upon TP treatment. Heterogeneous responses of nephron epithelial cells are observed after TP exposure, attributing differential susceptibility of cell subtypes to excessive reactive oxygen species and increased inflammatory responses. Moreover, TP disrupts vascular function by activating endothelial cell immunity and damaging fibroblasts. Severe immune cell damage and the activation of pro‐inflammatory Macro_C1 cells are also observed with TP treatment. Additionally, ligand‐receptor crosstalk analysis reveals that the SPP1 (osteopontin) signaling pathway targeting Macro_C1 cells is triggered by TP treatment, which may promote the infiltration of Macro_C1 cells to exacerbate renal toxicity. Overall, this study provides comprehensive information on the transcriptomic profiles and cellular composition of TP‐associated nephrotoxicity at single‐cell resolution, which can strengthen the understanding of the pathogenesis of TP‐induced nephrotoxicity and provide valuable clues for the discovery of new therapeutic targets to ameliorate TP‐associated nephrotoxicity.

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Single-cell transcriptome analysis reveals liver injury induced by glyphosate in mice

Cited by 12 publications

References 84 publications

Macrophage-driven cardiac inflammation and healing: insights from homeostasis and myocardial infarction

Macrophage-driven cardiac inflammation and healing: insights from homeostasis and myocardial infarction

Single-cell omics traces the heterogeneity of prostate cancer cells and the tumor microenvironment

A Single‐Cell Transcriptome Profiling of Triptolide‐Induced Nephrotoxicity in Mice

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