Single‐cell transcriptomics in human skin research: available technologies, technical considerations and disease applications

Theocharidis, Georgios; Tekkela, Stavroula; Veves, Aristidis; McGrath, John A.; Onoufriadis, Alexandros

doi:10.1111/exd.14547

Cited by 27 publications

(18 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These have been used successfully to decipher pathways of skin stromal cell development 11,12 and how wound healing across the life span is accompanied by an altered inflammatory milieu 13 . Alteration in skin landscape have similarly been studied in psoriaisis and atopic dermatitis 14 and cancer 15 , promising to contribute to the development of new therapeutic approaches to target these and other skin disorders. Skin-microbiota interactions have received much attention, with implications for normal homeostasis, immune system health and the response to infection 16,17 .…”

Section: Introductionmentioning

confidence: 99%

UVB modifies skin immune-stroma cross-talk and promotes effector T cell recruitment during crypticLeishmania donovaniinfection

Dey

Bocxlaer

et al. 2023

Preprint

View full text Add to dashboard Cite

Many parasites of significant public health importance assume skin residency without causing overt pathlogy. How immune and stromal cells respond to such cryptic infections and how exposure to UVB alters such responses in poorly understood. We combined scRNA-seq, spatial transcriptomics and inferential network analysis to address these questions in a model of cryptic skin infection by Leishmania donovani. In infected C57BL/6 mice, p-selectin and CXCL12 interactions dominate intercellular communication between leucocytes, fibroblast and endothelial cells, but effector T cell function remains muted. Following UVB exposure, increased numbers of IFNgamma+ CD4+ Th1 cells and NK cells enter the skin, communicating with stromal cells via CCL5-CCR5 and LFA-1-ICAM1/2. However, spatial mapping indicated that Th1 cells and macrophages occupied distinct niches after UVB exposure, likely limiting effector function. Our data provide the first holistic view of the immune landscape during cryptic L. donovani infection and demonstrate how UVB exposure fundamentally reshapes this response.

show abstract

Section: Introductionmentioning

confidence: 99%

UVB modifies skin immune-stroma cross-talk and promotes effector T cell recruitment during crypticLeishmania donovaniinfection

Dey

Bocxlaer

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Previous bulk RNA sequencing and microarray studies on skin inflammatory diseases have revealed dysregulation of inflammatory and barrier genes, but many cell-specific transcripts often remain masked in whole-skin transcriptomic analyses. In addition, it has been difficult to systematically understand the sophisticated interplay among cells in the skin ( 3 ). Sing-cell RNA-seq (scRNA-seq) can capture tissue transcripts at the single-cell resolution and effectively solve this problem ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it has been difficult to systematically understand the sophisticated interplay among cells in the skin ( 3 ). Sing-cell RNA-seq (scRNA-seq) can capture tissue transcripts at the single-cell resolution and effectively solve this problem ( 3 ). The experimental procedure of scRNA-seq mainly includes four steps: sample preparation, cell enrichment, library preparation, and data analysis ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Transcriptional Analysis Deciphers the Inflammatory Response of Skin-Resident Stromal Cells

Cui

2022

Front. Surg.

View full text Add to dashboard Cite

The skin is the outermost barrier of the body. It has developed a sophisticated system against the ever-changing environment. The application of single-cell technologies has revolutionized dermatology research and unraveled the changes and interactions across skin resident cells in the healthy and inflamed skin. Single-cell technologies have revealed the critical roles of stromal cells in an inflammatory response and explained a series of plausible previous findings concerning skin immunity. Here, we summarized the functional diversity of skin stromal cells defined by single-cell analyses and how these cells orchestrated events leading to inflammatory diseases, including atopic dermatitis, psoriasis, vitiligo, and systemic lupus erythematosus.

show abstract

“…including genomics [13], transcriptomics [14], metabolomics [15], microbiomics [16], and proteomics [17]. The effects of drugs on macaques have been explored using multi-omics analysis, greatly promoting biomedical research.…”

Section: Introductionmentioning

confidence: 99%

Multi-omics analysis reveals changes in tryptophan and cholesterol metabolism before and after sexual maturation in captive macaques

Liu

Wang

et al. 2022

Preprint

View full text Add to dashboard Cite

Rhesus macaques (Macaca mulatta, RMs) are widely used in sexual maturation studies due to their high genetic and physiological similarity to humans. However, judging sexual maturity in captive RMs based on blood physiological indicators, female menstruation, and male ejaculation behavior can be inaccurate. Here, we explored changes in RMs before and after sexual maturation based on multi-omics analysis and identified markers for determining sexual maturity. We found that differentially expressed microbiota, metabolites, and genes before and after sexual maturation showed many potential correlations. Specifically, genes involved in spermatogenesis (TSSK2, HSP90AA1, SOX5, SPAG16, and SPATC1) were up-regulated in male macaques, and significant changes in genes (CD36), metabolites (cholesterol, 7-ketolithocholic acid, and 12-ketolithocholic acid), and microbiota (Lactobacillus) related to cholesterol metabolism were also found, suggesting the sexually mature males have stronger sperm fertility and cholesterol metabolism compared to sexually immature males. In female macaques, most differences before and after sexual maturity were related to tryptophan metabolism, including changes in IDO1, IDO2, IFNGR2, IL1Β, IL10, L-tryptophan, kynurenic acid (KA), indole-3-acetic acid (IAA), indoleacetaldehyde, and Bifidobacteria, indicating that sexually mature females exhibit stronger neuromodulation and intestinal immunity than sexually immature females. Cholesterol metabolism-related changes (CD36, 7-ketolithocholic acid, 12-ketolithocholic acid) were also observed in female and male macaques. We explored changes before and after sexual maturation through a multi-omics approach and identified potential biomarkers for judging sexual maturity in RMs, including Lactobacillus (for males) and Bifidobacterium (for females), which is valuable for RM breeding and sexual maturation research.

show abstract

Single‐cell transcriptomics in human skin research: available technologies, technical considerations and disease applications

Cited by 27 publications

References 107 publications

UVB modifies skin immune-stroma cross-talk and promotes effector T cell recruitment during crypticLeishmania donovaniinfection

UVB modifies skin immune-stroma cross-talk and promotes effector T cell recruitment during crypticLeishmania donovaniinfection

Single-Cell Transcriptional Analysis Deciphers the Inflammatory Response of Skin-Resident Stromal Cells

Multi-omics analysis reveals changes in tryptophan and cholesterol metabolism before and after sexual maturation in captive macaques

Contact Info

Product

Resources

About