Single count of radicle emergence, DNA replication during seed germination and vigour in alfalfa seed lots

Cheshmi, Mostafa; Khajeh-Hosseini, M.

doi:10.15258/sst.2020.48.3.05

Cited by 8 publications

(3 citation statements)

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“…High-vigor seeds show rapid and uniform germination and emergence performance, especially under stressful environmental conditions [9,10]. In contrast, low-vigor seeds generally caused poor seedling emergence and resulted in serious yield and economic losses, as previously found for alfalfa (Medicago sativa L.) [17], white clover (Trifolium repens L.) [18], Festuca sinensis [19], and many other plant species [20][21][22]. Therefore, more attention should be paid to seed vigor and its testing for grass and forage species.…”

Section: Introductionmentioning

confidence: 75%

“…The RE test also estimates seed vigor, as reflected by field emergence (FE), for many grass and forage species. For example, Khajeh-Hosseini and Cheshmi found that a single count of RE carried out at 24 h for alfalfa could be used as a vigor test and was highly predictive of FE [17]. Likewise, a single count of RE at 32 h also effectively ranks the seed vigor level of seed lots for forage grass pearl millet (Pennisetum glaucum) [38].…”

Section: Introductionmentioning

confidence: 99%

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Siberian Wildrye (Elymus sibiricus) Seed Vigor Estimation for the Prediction of Emergence Performance under Diverse Environmental Conditions

Tao,

Xing,

Meng

et al. 2024

Agronomy

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Seed vigor is an important aspect of seed quality. It is critical to predict seed vigor and plant seedling emergence under diverse environmental conditions using the laboratory vigor test. Accordingly, laboratory experiments were conducted to determine the standard germination (SG), early individual counts of radicle emergence (RE), mean germination time (MGT), and vigor index (VI) for 16 commercially available seed lots of Siberian wildrye (Elymus sibiricus), an economically and ecological important grass species. The field emergence (FE) for seed lots was explored using a three-year field trial from 2021 to 2023. Meanwhile, pot experiments were carried out to determine the seedling emergence performance under different environmental conditions, including control, drought, and salinity stress. The correlation and regression analysis were done to investigate the links between laboratory vigor test with emergence performance under both field and pot conditions. The results showed that the SG of 16 seed lots were high, similar, and did not differ significantly between seed lots. However, the seedling emergence performance (emergence percentage, seedling dry weight, and simplified vigor index) differed significantly between seed lots under both field and pot conditions. The SG was not significantly correlated with seedling emergence performance under either the field or pot conditions. The counts of RE at selected timing, MGT, and VI showed a significant relationship with seed vigor as reflected by seedling emergence performance under diverse environmental conditions, and we found that RE at 108 h was highly predictive of seed vigor and seedling emergence. The RE at 108 h and VI were positively related to seed vigor, while MGT was negatively related. The seed lots with low vigor had lower RE at 108 h and VI but longer MGT. Overall, both RE at 108 h, MGT, and VI can be used to estimate seed vigor and predict seedling emergence under different environmental conditions, and we highly recommend RE at 108 h as a quick, precise, and convenient vigor test and early warning sign for seed storage of E. sibiricus. These results will enable seed researchers, plant breeders, farmers, and government program directors to target higher seed vigor more effectively for E. sibiricus and similar grass species.

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Section: Introductionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Siberian Wildrye (Elymus sibiricus) Seed Vigor Estimation for the Prediction of Emergence Performance under Diverse Environmental Conditions

Tao,

Xing,

Meng

et al. 2024

Agronomy

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“…Tao et al found that there was an advantage in using high-vigor CMV seeds to achieve better seedling emergence, whereas low-vigor seeds caused limited seedling emergence [18]. The selection of high-vigor seeds has also been proven to be helpful for seedling emergence in other forage legumes, such as alfalfa (Medicago sativa L.) [19], white clover (Trifolium repens L.) [20], and birdsfoot trefoil (Lotus corniculatus L.) [21]. Thus, more attention should be paid to seed-vigor testing for forage legumes.…”

Section: Introductionmentioning

confidence: 99%

Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions

Tao

Chen

Bai

et al. 2023

Plants

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Seed vigor is an important aspect of seed quality. High-vigor seeds show rapid and uniform germination and emerge well, especially under adverse environmental conditions. Here, we determined hydrotime model parameters by incubating seeds at different water potentials (0.0, −0.2, −0.4, −0.6, and −0.8 MPa) in the laboratory, for 12 seed lots of Chinese milk vetch (Astragalus sinicus) (CMV), a globally important legume used as forage, green manure, and a rotation crop. Pot experiments were conducted to investigate the seedling emergence performance of 12 CMV seed lots under control, water stress, salinity stress, deep sowing, and cold stress conditions. Meanwhile, the field emergence performance was evaluated on two sowing dates in June and October 2022. Correlation and regression analyses were implemented to explore the relationships between hydrotime model parameters and seedling emergence performance under various environmental conditions. The seed germination percentage did not differ significantly between seed lots when seeds were incubated at 0.0 MPa, whereas it did differ significantly between seed lots at water potentials of −0.2, −0.4, and −0.6 MPa. The emergence percentage, seedling dry weight, and simplified vigor index also differed significantly between the 12 seed lots under various environmental conditions. Ψb(50) showed a significant correlation with germination and emergence performance under various environmental conditions; however, little correlation was observed between θH or σφb and germination and emergence. These results indicate that Ψb(50) can be used to estimate seed vigor and predict seedling emergence performance under diverse environmental conditions for CMV and similar forage legumes. This study will enable seed researchers, plant breeders, and government program directors to target higher seed vigor more effectively for forage legumes.

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Single count of radicle emergence and mean germination time estimate seed vigour of Chinese milk vetch (Astragalus sinicus)

Tao

Sun

Zhang

et al. 2022

Seed Science and Technology

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This research explored the relationship between a single count of radicle emergence (RE) and mean germination time (MGT) with seed vigour for Chinese milk vetch (Astragalus sinicus) (CMV), a globally important legume used as a forage, green manure, and rotation crop. Fourteen seed lots of CMV were used to determine standard germination (SG), RE and MGT in the laboratory. Field experiments were conducted at two sites to measure the emergence performance including field emergence (FE), seedling dry weight (SDW) and simplified vigour index (SVI). The SG ranged from 89.0 to 95.5% and was not significantly correlated with field performance at either of two sites. However, both a single count of RE at 20 hours and MGT were highly predictive of FE, SDW and SVI at both sites (R2 ranged from 0.612 to 0.897 for RE; 0.604 to 0.870 for MGT). The RE was positively related to field performance while MGT was negatively related. The seed lots with lower RE at 20 hours had lower FE and higher MGT. Overall, both RE at 20 hours and MGT are suitable indices for the vigour evaluation of CMV. However, a single count of RE is more accurate, quick and convenient.

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Single count of radicle emergence, DNA replication during seed germination and vigour in alfalfa seed lots

Cited by 8 publications

References 0 publications

Siberian Wildrye (Elymus sibiricus) Seed Vigor Estimation for the Prediction of Emergence Performance under Diverse Environmental Conditions

Siberian Wildrye (Elymus sibiricus) Seed Vigor Estimation for the Prediction of Emergence Performance under Diverse Environmental Conditions

Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions

Single count of radicle emergence and mean germination time estimate seed vigour of Chinese milk vetch (Astragalus sinicus)

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