Single-digit Salmonella detection with the naked eye using bio-barcode immunoassay coupled with recombinase polymerase amplification and a CRISPR-Cas12a system

Abstract

The ability to visually detect low numbers of Salmonella in food samples is highly valuable but remains a challenge. Here we present a novel platform for ultrasensitive and visual detection...

CRISPR‐Cas‐based detection for food safety problems: Current status, challenges, and opportunities

“…Also, for shrimp samples, a homemade UV device was used as signal output for identifying infected seafood with NaOH‐based DNA crude extraction (Zhang et al., 2020). The bio‐barcode immunoassay and CRISPR‐Cas12a were integrated into a single reaction system for detecting S. Typhimurium at the single‐digit level within 60 min (Cai et al., 2021).…”

Development of a Rapid and Efficient RPA-CRISPR/Cas12a Assay for Mycoplasma pneumoniae Detection

“…Additionally, cleavage results of CRISPR/Cas12a system can be visualized using lateral flow biosensor (LFB) or fluorescence reader by introducing ssDNA reporter labeled with fluorophore and biotin or alternatively with fluorophore and quencher into an in vitro reaction (Xiong et al, 2020;Zhu et al, 2021). Now, CRISPR/Cas12a detection integrated with RPA has been successfully applied in rapid and accurate detection of various pathogens including viral pathogens (such as SARS-CoV-2, human metapneumovirus, human papillomavirus and African swine fever; Bai et al, 2019;Yin et al, 2020;Qian et al, 2021;Sun et al, 2021;Talwar et al, 2021), fungal pathogens (for example Elsinoë fawcettii; Shin et al, 2021), Bacterial pathogens (such as Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, Vibrio parahaemolyticus, Salmonella sp., Xanthomonas arboricola and Pseudomonas aeruginosa; Cai et al, 2021;Liu et al, 2021;Luo et al, 2021). In this study, RPA coupled with CRISPR/Cas12a (RPA-CRISPR/Cas12a) has been developed for MP detection.…”

Recent Developments in Innovative Magnetic Nanoparticles-Based Immunoassays: From Improvement of Conventional Immunoassays to Diagnosis of COVID-19

“…Indeed, MNPs have been effectively applied to rapid and highly sensitive detection for a broad range of target analytes from viruses [7,8] and bacteria [9,10] to food allergen [11,12]. Furthermore, it is worth noting that the latest studies have incorporated MNPs-based immunoassays with a CRISPR (clustered regularly interspaced short palindromic repeats)/Cas system, which is an attractive method for specific recognition of infectious bacteria and viruses [13,14]. For example, Kim et al [13] applied MNPs to a CRISPR/Cas system combined with surface-enhanced Raman scattering (SERS) assay to detect multidrug-resistant (MDR) bacteria.…”