Single domain antibodies derived from ancient animals as broadly neutralizing agents for SARS-CoV-2 and other coronaviruses

Lim, Hui Ting; Kok, Boon Hui; Lim, Chin Peng; Majeed, Abu Bakar Abdul; Leow, Chiuan Yee; Leow, Chiuan Herng

doi:10.1016/j.bea.2022.100054

Cited by 5 publications

(2 citation statements)

References 154 publications

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“…We particularly focus on studies describing innovative optimization approaches and those including in vivo data. As multiple reviews on sdAbs targeting SARS-CoV-2 have been published in recent years ( Bessalah et al, 2021 ; Chen et al, 2021 ; Czajka et al, 2021 ; Lim et al, 2022 ; Zebardast et al, 2022 ; Bhattacharya et al, 2023 ; Feng and Wang, 2023 ), our endeavor has not been to give an overview of recent literature, but to integrate the latest findings on optimization techniques described for CoV and Influenza virus targeting sdAbs.…”

Section: Sdabs Targeting Respiratory Zoonotic Virusesmentioning

confidence: 99%

Next generation single-domain antibodies against respiratory zoonotic RNA viruses

Swart,

Van Gelder,

De Haan

et al. 2024

Front. Mol. Biosci.

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The global impact of zoonotic viral outbreaks underscores the pressing need for innovative antiviral strategies, particularly against respiratory zoonotic RNA viruses. These viruses possess a high potential to trigger future epidemics and pandemics due to their high mutation rate, broad host range and efficient spread through airborne transmission. Recent pandemics caused by coronaviruses and influenza A viruses underscore the importance of developing targeted antiviral strategies. Single-domain antibodies (sdAbs), originating from camelids, also known as nanobodies or VHHs (Variable Heavy domain of Heavy chain antibodies), have emerged as promising tools to combat current and impending zoonotic viral threats. Their unique structure, coupled with attributes like robustness, compact size, and cost-effectiveness, positions them as strong alternatives to traditional monoclonal antibodies. This review describes the pivotal role of sdAbs in combating respiratory zoonotic viruses, with a primary focus on enhancing sdAb antiviral potency through optimization techniques and diverse administration strategies. We discuss both the promises and challenges within this dynamically growing field.

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Section: Sdabs Targeting Respiratory Zoonotic Virusesmentioning

confidence: 99%

Next generation single-domain antibodies against respiratory zoonotic RNA viruses

Swart,

Van Gelder,

De Haan

et al. 2024

Front. Mol. Biosci.

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“…Single-domain antibodies (sdAbs) are a kind of unconventional antibodies of the immune system present in camels or cartilaginous sh, which only retain the variable region of the heavy chain of antibodies with antigen-binding activity (Lim et al 2022;Liu et al 2023a However, one thing should be noted that single-domain antibodies usually have low expression yields, which has been restricting their application in small hazards detection.…”

Section: Introductionmentioning

confidence: 99%

Heterologous Expression, Characterization, and Fish Matrix Performance of a Shark-Derived Single-Domain Antibody Fusion Protein against Enrofloxacin

jia,

Liu,

Lin

et al. 2023

Preprint

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The abuse of enrofloxacin (ENR) in aquaculture and the lack of monitoring of other metabolites may lead to unknown harmful effects on human health. In this study, a shark-derived single-domain antibody fusion protein 2E6-SUMO, which was specific to ENR, was heterologously expressed. The indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) using 2E6-SUMO as the recognition element was established to detect ENR in fish samples. The results showed that the 2E6-SUMO fusion protein has good thermal performance compared to traditional IgG antibodies. The half-maximal inhibitory concentration (IC50) of 2E6-SUMO to ENR was determined as 42.08 ng/mL, with a detection limit of 3.84 ng/mL and a linear range (IC20 − 80) of 9.94–376.17 ng/mL, respectively. In our experiments, the ENR-negative fish matrix did not seriously interfere with the recognition of the 2E6-SUMO to ENR, with the matrix index between 3.31% and 6.85%. The recovery experiment indicated that the recovery rates of spiked samples ranged between 83.33% and 123.06%. Our study broadens the application scenario of shark-derived single-domain antibodies, providing new immunoassay method for detecting ENR.

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Shark VNAR phage display libraries: An alternative source for therapeutic and diagnostic recombinant antibody fragments

Manoutcharian

Gevorkian

2023

Fish & Shellfish Immunology

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Single domain antibodies derived from ancient animals as broadly neutralizing agents for SARS-CoV-2 and other coronaviruses

Cited by 5 publications

References 154 publications

Next generation single-domain antibodies against respiratory zoonotic RNA viruses

Next generation single-domain antibodies against respiratory zoonotic RNA viruses

Heterologous Expression, Characterization, and Fish Matrix Performance of a Shark-Derived Single-Domain Antibody Fusion Protein against Enrofloxacin

Shark VNAR phage display libraries: An alternative source for therapeutic and diagnostic recombinant antibody fragments

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