Single etalon design for two-stage cross-axis VIPA spectroscopy

Abstract

Two-stage cross axis VIPA spectrometers have been widely used in Brillouin microscopy since they provide single shot spectral measurements at high throughput and extinction. However, this spectrometer configuration presents challenges such as size, cost and alignment difficulties between the two cascaded etalons. Here, we present a cross-axis VIPA spectrometer that implements a single etalon, using a light recirculation architecture to achieve the multistage configuration.

Brillouin Spectroscopy: From Biomedical Research to New Generation Pathology Diagnosis

“…The acquisition times were reduced by several orders of magnitude down to Raman spectroscopy’s typical acquisition times [ 37 ]. Technical improvements of VIPA spectrometers have continued to enhance performances and simplify the optical setup within the spectrometer [ 38 ].…”

Multivariate analysis of Brillouin imaging data by supervised and unsupervised learning

“…The main attraction of the technique is the ability to probe mechanical properties of structurally complex samples, especially of biological nature with optical resolutions, in a non-contact manner. Experimentally, this was first demonstrated by Koski et al 1 The fusion of spectroscopy and imaging has advanced the technique into a powerful, hyperspectral (HS) modality, which has been used to study a wide range of samples, including cells, 2 embryos, 3 biofilms, 4 human cornea, 5 tumours 6 and so on, covered in greater detail in the review by Meng et al 7 While the development of the instrument is reaching a state of maturity, with ongoing effort focusing on faster, more efficient designs to facilitate in vivo applications, [8][9][10] more work is still needed on the analysis and interpretation of the hyperspectral datasets. 11 Currently, the most common analysis method is to fit spectral features individually, usually by a single peak in each spectrum to extract the average Brillouin shift and linewidth information, which are then mapped to achieve imaging.…”

“…human cornea [5], tumours [6] etc., covered in greater detail in the review by Meng et al [7]. While the development of the instrument is reaching a state of maturity, with ongoing effort focusing on faster, more efficient designs to facilitate in vivo applications [8,9,10], more work is still needed on the analysis and interpretation of the hyperspectral datasets [11]. Currently, the most common analysis method is to fit spectral features individually, usually by a single peak in each 1 arXiv:2009.07220v1 [eess.IV] 15 Sep 2020 spectrum to extract the average Brillouin shift and linewidth information, which are then mapped to achieve imaging.…”

Brillouin Light Scattering Microspectroscopy for Biomedical Research and Applications: introduction to feature issue

“…To achieve sufficient finesse to resolve the BLS spectra in non-transparent samples, two such elements are often placed in a cross-dispersion configuration, such that the spectrometer as a whole is spatially quite expansive. Here Fiore et al [1] present a novel method of using only a single VIPA, namely by passing the light through it twice (with the second time the beam having been rotated by 90°). The result is that they effectively achieve a cross-dispersion setup using only a single such element, which holds potential for saving costs and compaction of BLS spectrometers.…”