Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats

Li, Rui; Wu, Jiang; Lin, Zhenkun; Nangle, Matthew R.; Li, Yang; Cai, Pingtao; Liu, Dan; Ye, Libin; Xiao, Zecong; He, Chaochao; Ye, Jingjing; Zhang, Hongyu; Zhao, Ying‐Zheng; Wang, Jian; Li, Xiaokun; He, Yan; Ye, Qingsong; Xiao, Jian

doi:10.1016/j.expneurol.2016.10.015

Cited by 59 publications

(56 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Crushed injuries of sciatic nerves were administered to the Diabetes group according to a previously described method [35]. In brief, after an appropriate incision of the skin, the gluteal musculature was separated in order to expose the right sciatic nerve.…”

Section: Methodsmentioning

confidence: 99%

“…The fabrication of ultra-thin (50nm) sections of sciatic nerves, image capturing procedure, and quantification of morphometric data were performed as previously described [35]. …”

Section: Methodsmentioning

confidence: 99%

“…Standard immunohistochemistry procedures are described previously [35], and the following primary antibodies were used: anti-MBP (myelin marker, 1μg/ml, ab40390, Abcam) and anti-NF200 (heavy subunit of neurofilament for axons, 0.01μg/ml, ab4680, Abcam), anti-GFAP (SCs, 0.67μg/ml, sc-6170, Santa) and anti-Ki67 (marker for cell proliferation, 1μg/ml, ab15580, Abcam). All images were captured on an inverted confocal microscope (Nikon) or a Nikon Eclipse 80i fluorescence microscope.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Heparin-Poloxamer Thermosensitive Hydrogel Loaded with bFGF and NGF Enhances Peripheral Nerve Regeneration in Diabetic Rats

et al. 2018

Biomaterials

Self Cite

199

140

View full text Add to dashboard Cite

Peripheral nerve injury (PNI) is a major burden to society with limited therapeutic options, and novel biomaterials have great potential for shifting the current paradigm of treatment. With a rising prevalence of chronic illnesses such as diabetes mellitus (DM), treatment of PNI is further complicated, and only few studies have proposed therapies suitable for peripheral nerve regeneration in DM. To provide a supportive environment to restore structure and/or function of nerves in DM, we developed a novel thermo-sensitive heparin-poloxamer (HP) hydrogel co-delivered with basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) in diabetic rats with sciatic nerve crush injury. The delivery vehicle not only had a good affinity for large amounts of growth factors (GFs), but also controlled their release in a steady fashion, preventing degradation in vitro. In vivo, compared with HP hydrogel alone or direct GFs administration, GFs-HP hydrogel treatment is more effective at facilitating Schwann cell (SC) proliferation, leading to an increased expression of nerve associated structural proteins, enhanced axonal regeneration and remyelination, and improved recovery of motor function (all p<0.05). Our mechanistic investigation also revealed that these neuroprotective and neuroregenerative effects of the GFs-HP hydrogel may be associated with activations of phosphatidylinositol 3 kinase and protein kinase B (PI3K/Akt), janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3), and mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling pathways. Our work provides a promising therapy option for peripheral nerve regeneration in patients with DM.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The fabrication of ultra-thin (50nm) sections of sciatic nerves, image capturing procedure, and quantification of morphometric data were performed as previously described [35]. …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Heparin-Poloxamer Thermosensitive Hydrogel Loaded with bFGF and NGF Enhances Peripheral Nerve Regeneration in Diabetic Rats

et al. 2018

Biomaterials

Self Cite

199

140

View full text Add to dashboard Cite

show abstract

“…Using a 4/0 chromic gut suture, the muscle layers were re-connected while 4/0 silk sutures were applied to close the skin. After surgery, the rats were monitored during their recovery from the anesthetic state under heating lamps and were subsequently left to feed and drink ad libitum (Chauderlier et al, 2017[7]; Li et al, 2017[20]).…”

Section: Methodsmentioning

confidence: 99%

Comparison of melatonin and curcumin effect at the light and dark periods on regeneration of sciatic nerve crush injury in rats

Kasmaie

Jahromi

Gazor

et al. 2019

EXCLI Journal; 18:Doc653; ISSN 1611-2156

View full text Add to dashboard Cite

“…It had been demonstrated that neurotrophic factors help to stimulate and support peripheral nerve repair . Among these factors, NGF participates in the regulation, activation, protection, and neutrition of nerve cells . Previous studies have demonstrated that it may promote peripheral nerve regeneration efficiently, furthermore, it appears to promote myelin repair .…”

Section: Introductionmentioning

confidence: 99%

Efect of longitudinally oriented collagen conduit combined with nerve growth factor on nerve regeneration after dog sciatic nerve injury

Yao

Cui²,

Zhao

et al. 2017

J Biomed Mater Res

View full text Add to dashboard Cite

The research on artificial nerve conduits has become a focus of study in peripheral nerve reconstruction so as a possible replacement for the treatment of autologous nerve grafts in clinics. In this study, we used longitudinally oriented collagen conduit (LOCC) combined with nerve growth factor (NGF) to reconstruct long distance of sciatic nerve defects (35 mm) in adult dog model. The long term follow-up evaluation demonstrated that the LOCC/NGF conduit allowed functional and morphological nerve regeneration at the transection site of the injured sciatic nerve. Furthermore, the functional study confirmed that when NGF was loaded onto LOCC it promoted a better recovery of regenerated axons than LOCC alone. The gastrocnemius muscle mass in the LOCC/NGF group was significantly greater than in the LOCC alone group. The results indicated that when LOCC conduit combined with NGF it would provide a preferential environment for sciatic nerve regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2131-2139, 2018.

show abstract

Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats

Cited by 59 publications

References 40 publications

Heparin-Poloxamer Thermosensitive Hydrogel Loaded with bFGF and NGF Enhances Peripheral Nerve Regeneration in Diabetic Rats

Heparin-Poloxamer Thermosensitive Hydrogel Loaded with bFGF and NGF Enhances Peripheral Nerve Regeneration in Diabetic Rats

Comparison of melatonin and curcumin effect at the light and dark periods on regeneration of sciatic nerve crush injury in rats

Efect of longitudinally oriented collagen conduit combined with nerve growth factor on nerve regeneration after dog sciatic nerve injury

Contact Info

Product

Resources

About