2015
DOI: 10.1038/micronano.2015.31
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Single living cell manipulation and identification using microsystems technologies

Abstract: The paper presents the principles and the results of the implementation of dielectrophoresis for separation and identification of rare cells such as circulation tumor cells (CTCs) from diluted blood specimens in media and further label-free identification of the origins of separated cells using radio-frequency (RF) imaging. The separation and the identification units use same fabrication methods which enable system integration on the same platform. The designs use the advantage of higher surface volume ratio w… Show more

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Cited by 21 publications
(14 citation statements)
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“…AC‐based DEP requires nonuniform electric field which, in general, is created using conductive electrodes . The DEP force on a spherical polarizable microentity under nonuniform electric field while immersed in a conductive medium is defined by : F DEP =2πεnormalmr3 Re f cm E rms 2where ε m is the permittivity of the medium, r is the radius of the microentity, E is the electric field, and Re[ f CM ] is the real part of Clausius–Mossotti factor that depends on the complex permittivities of the microentity and the suspended medium and is defined as: f cm =εpεmεp+2εmwhere ε * is the complex permittivity that is a function of the dielectric constant ε , the angular frequency ω , and the real conductivity σ and is given by: ε*=εjσωwhere j is the imaginary unit, j=1…”
Section: Methodsmentioning
confidence: 99%
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“…AC‐based DEP requires nonuniform electric field which, in general, is created using conductive electrodes . The DEP force on a spherical polarizable microentity under nonuniform electric field while immersed in a conductive medium is defined by : F DEP =2πεnormalmr3 Re f cm E rms 2where ε m is the permittivity of the medium, r is the radius of the microentity, E is the electric field, and Re[ f CM ] is the real part of Clausius–Mossotti factor that depends on the complex permittivities of the microentity and the suspended medium and is defined as: f cm =εpεmεp+2εmwhere ε * is the complex permittivity that is a function of the dielectric constant ε , the angular frequency ω , and the real conductivity σ and is given by: ε*=εjσωwhere j is the imaginary unit, j=1…”
Section: Methodsmentioning
confidence: 99%
“…AC-based DEP requires nonuniform electric field which, in general, is created using conductive electrodes [31,32]. The DEP force on a spherical polarizable microentity under nonuniform electric field while immersed in a conductive medium is defined by [25]:…”
Section: Theoretical Background and Separation Approachmentioning
confidence: 99%
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“…The generated inhomogeneous electric field induces lateral motion on cells because of dielectrophoresis (DEP). The DEP is defined as the motion of neutral and polarizable particles or cells suspended in a conductive medium under the effect of inhomogeneous electric field [36][37][38]. It is a label-free manipulation method with low impact on the cellular integrity [38].…”
Section: Living Cells Manipulationmentioning
confidence: 99%
“…Although much research has been conducted about the use of DEP for biological and medical purposes, such as the isolation of rare circulating tumour cells at concentrations lower than one in a million , there have been relatively few studies on its potential uses for IVF. There is however, great potential for its application, as it is an extremely effective sorting technique for rare cells.…”
Section: Introductionmentioning
confidence: 99%